几个沙棘品种抗逆性试验研究

根据寒温带半干旱地区的自然条件,选择耐盐碱性、耐寒性、耐水湿三项指标在温室盆钵内对HF-14、HF-4两个沙棘优良无性系及对照品种(楚伊)进行人工模拟抗逆性测定。其结果为:3个无性系对盐碱的耐受性均较强并无明显差别,可在中度以下盐碱地上栽培;3个无性系具有一定的耐旱能力,但在长期持续干旱条件下,生长量明显下降,生长势衰弱,增加感病机会,应给予灌溉,3个无性系的耐旱次序为HF-4>HF-14>楚伊;3个无性系对水湿条件的耐受力基本相同,在长期积水的条件下,根系不发育,生长受到抑制,栽植沙棘不应选择低洼内涝,排水不良的地块。     

原料和热解温度对生物油分子蒸馏分离特性的影响

针对生物油组分复杂,难以直接应用的问题,该研究开展了不同生物油的分子蒸馏馏分分布规律的研究,并考察了不同原料和温度对生物油分子蒸馏分离特性的影响。热解液化试验结果表明,生物油组分以酸、醛、酮、酚、糖为主。随着热解温度的升高,松木生物油中轻质组分产率由21%不断降低至11%,而秸秆生物油中轻质组分产率稳定在20%左右。高温生物油各组分的平均分子自由程两极化程度加强,600℃制备轻质油蒸出比例最高,可以达到92%（松木）和86%（秸秆）。酚类化合物中酚羟基的数量可以显著影响其分离特性,较高的热解温度促进了酚羟基的产生,从而使酚类物质从蒸出部分（distillation fraction, DF）向残留部分（residual fraction, RF）中转移。分子蒸馏技术能够实现对不同生物油的有效分离,得到的DF中主要包含酸、酮和小分子酚类,RF则以糖和大分子酚为主,除了羟乙醛和苯并呋喃等化合物外,生物油中的大部分化合物的富集程度都可以达到90%以上。该研究可为快速热解生物油的分离及其后续提质研究提供一定的参考。     

水稻骨干亲本育种价值分析

在介绍粳稻丰锦、辽粳5号、盐粳32、盐粳68等来源及特点的基础上,以配合力为指标,分析了这4个品种的产量性状的遗传潜势,结合系谱分析,论证了骨干亲本的育种价值,并探讨了骨干亲本的利用方式。     

Mutation Techniques for Gene Discovery and Crop Improvement

One of the most important breakthroughs in the history of genetics ）was the discovery that mutations can be artificially induced in organisms （van Harten, 1998）. Artificially induced mutations, by physical and chemical mutagens, have greatly advanced the understanding of genetics of higher organisms. Starting in the late 1960＇s, the International Atomic Energy Agency （IAEA） and the Food and Agriculture Organization （FAO） of the United Nations sponsored extensive research on mutation induction and their application to breeding of food and indnstrial crops that resulted in the introduction of new varieties of rice, wheat, barley, apples, citrus, sugar cane, banana, and others （more than 2 500 officially released new varieties are to be found in the FAO/IAEA Mutant Varieties Database） （hitp：//www-mvd.iaea.org/MVD/default.htm）. However, the usefulness of mutation techniques has been underappreciated in research communities, particularly during the last decade, when more and more researchers and breeders were rushing into molecular marker techniques and transgenic plants. In this paper, after a brief review of the past accomplishments of mutation induction and its application, we discuss the uniqueness of induced mutations in gene discovery and how to integrate induced mutants into functional genomics programs;     

西洋小南瓜新品种白沙蜜栗一号的选育

白沙蜜栗一号小南瓜是汕头市白沙蔬菜原种研究所以NY-8为母本、TG-6为父本配制而成的一代杂种.早中熟,植株生长势强,第一雌花着生于主蔓7～9节,瓜扁圆形,果皮和瓜瓤橙红色、有光泽,横径16.5～17.8 cm、纵径15.2～15.8 cm,单瓜重约1.5 kg;座果率高,品质粉质、香甜,抗白粉病和病毒病;每667 m2产量1 500～1 800 kg.     

In Vitro and In Vivo Dendritic Cell Immune Stimulation Effect of Low Molecular Weight Fucoidan from New Zealand Undaria pinnatifida

Low molecular weight fucoidan (LMWF) has been reported to have immunomodulation effects through the increase of the activation and function of macrophages. In this study, the regulating effect of LMWF from Undaria pinnatifida grown in New Zealand on dendritic cells (DCs) was investigated. We discovered that LMWF could stimulate DCs’ maturation and migration, as well as CD4+ and CD8+ T cells’ proliferation in vitro. We proved that this immune promoting activity is activated through TLR4 and its downstream MAPK and NF–κB signaling pathways. Further in vivo (mouse model) investigation showed that LMWF has a strong immunological boosting effect, such as facilitating the proliferation of immune cells and increasing the index of immune organs. These findings suggest that LMWF has a positive immunomodulatory effect and is a promising candidate to supplement cancer immunotherapy.     

Neoechinulin A as a Promising SARS-CoV-2 Mpro Inhibitor: In Vitro and In Silico Study Showing the Ability of Simulations in Discerning Active from Inactive Enzyme Inhibitors

The COVID-19 pandemic and its continuing emerging variants emphasize the need to discover appropriate treatment, where vaccines alone have failed to show complete protection against the new variants of the virus. Therefore, treatment of the infected cases is critical. This paper discusses the bio-guided isolation of three indole diketopiperazine alkaloids, neoechinulin A (1), echinulin (2), and eurocristatine (3), from the Red Sea-derived Aspergillus fumigatus MR2012. Neoechinulin A (1) exhibited a potent inhibitory effect against SARS-CoV-2 M^pro with IC₅₀ value of 0.47 μM, which is comparable to the reference standard GC376. Despite the structural similarity between the three compounds, only 1 showed a promising effect. The mechanism of inhibition is discussed in light of a series of extensive molecular docking, classical and steered molecular dynamics simulation experiments. This paper sheds light on indole diketopiperazine alkaloids as a potential structural motif against SARS-CoV-2 M^pro. Additionally, it highlights the potential of different molecular docking and molecular dynamics simulation approaches in the discrimination between active and inactive structurally related M^pro inhibitors.     

等离子体处理对亚麻籽胶结构和功能特性的影响

为了揭示等离子体技术对亚麻籽胶的物理改性效应,该研究以5 mg/m L亚麻籽胶溶液为对象,比较了不同等离子体处理时间(0～120 s)对亚麻籽胶结构和功能特性的影响规律。结果发现,随着等离子体处理时间的延长,亚麻籽胶溶液p H值、Zeta电位绝对值和平均分子量逐渐减小,而对亚麻籽胶的多糖骨架结构和单糖组成无明显影响。等离子体处理降低亚麻籽胶的炭化和聚合反应温度、表观黏度和界面吸附能力。冷场扫描电镜结合稳定性指数(Turbiscan Stability Index, TSI)结果显示,0～15 s等离子体处理在不明显降低亚麻籽胶中酚类化合物含量和体外抗氧化活性的同时,明显改善了亚麻籽胶构建亚麻籽油乳液的稳定性(P0.05)。因此,等离子体作为一种非热加工技术,能够用于亚麻籽胶的适度改性研究,以拓宽其在健康食品领域中的应用前景。     

养殖废水处理设施自动化智能化运营与管控

为实现养殖废水处理设施自动化智能化运营与管控,本文以日产废水250 m³的某规模化养殖场废水处理设施为例,通过配置视频监控系统和在线监控设备,并设置预警预报功能,来展示自动化智能化运营与管控效果。实时监控结果表明,污泥浓度、DO、pH值等关键运行参数均控制在合理范围,分别为4 000~7 000 mg·L^-1、1.5 mg·L^-1（一级好氧池）和3~5 mg·L^-1（二级好氧池）、6.5~8.5;两级AO出水水质COD、氨氮均达标,分别为70 mg·L^-1以下和10 mg·L^-1以下,保障了设施运行效果,出水水质稳定达标,规避了环境风险。通过视频监控、在线监测、预警预报等功能,可有效监控废水处理设施每个处理环节运行状态,做到及时发现问题和解决问题,从而提高设施的管控效果和效率,并降低运营人员的投入,有效降低运营费用。实际运营结果表明,废水处理设施实施自动化智能化运营与管控,既能有效提高管控效果和效率,最大程度规避环境风险,又能降低运营成本,从而实现降本增效。     

异源八倍体小黑麦劲松81在贵州的产量表现

异源八倍体小黑麦劲松81于2006年通过贵州省农作物品种审定委员会审定。该品种具有矮秆、高产、稳产、易脱粒、结实率高、饱满度好,营养品质优异,对白粉病免疫,适应性广,再生能力强,可在早春割苗作饲料,粮饲兼用等优点。在中等、中下等肥力旱地中其区域试验平均产量226.14~251.6 kg/667m2,比劲松49(CK)增产12.84%~16.63%,居参试品种之首。生产试验平均产量258.5 kg/667m2,最高产量达348 kg/667m2,比CK增产17.66%,适宜在贵州海拔为1000~2300 m的高寒山区、半山区,中部麦区的中、下等肥力旱地以及省外生态环境近似的地区种植。