A MULTI -OBJECTIVE FUZZY DECISION -MAKING APPROACH FOR POWER NETWORK PLANNING UNDER UNCERTAINTY

This paper proposes a multi - objective fuzzy decision - making approach for power network planning under uncertainty ,which simutaneously considers not only the least investment cost,the minimum power loss,the maximum reliability,and the least environmental impacts, but also the uncertainty about the future load growth and the capital investment availability. The validity and effectiveness of the proposed approach is verified with numerical examples.     

Mechanism of Fuzilizhong decoction alleviating inflammatory damage of rats with non-alcoholic fatty liver disease

AIM To observe the effect of Fuzilizhong decoction on the inflammatory damage of non-alcoholic fatty liver disease （NAFLD） rats and to explore its mechanism. METHODS SPF male SD rats were randomly divided into 6 groups： control group, model group, high dose （20 mg·kg^-1·d^-1）, middle dose （10 mg·kg^-1·d^-1）, low dose （5 mg·kg^-1·d^-1） Fuzilizhong decoction group and Yishanfu （30 mg·kg^-1·d^-1）group, 8 rats in each group. A NAFLD rat modelwas established by intragastric administration of fat emulsion for 4 weeks. Then the drug was given for 4 weeks in each treatment group. HE staining was performed to observe the histopathological changes of the rat liver.The serum levels of interleukin-2（IL-2）, IL-6 and tumor necrosis factor-α（TNF-α） were measured by ELISA. The expression of toll like receptor 4（TLR4） and NF-κB p65 in liver tissues at mRNA and protein levels was determined by RT-qPCR and Western bolt,respectively. RESULTS Compared with control group, the inflammatory damage of liver tissue was more serious, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression TLR4 and NF-κB p65 in liver tissues were significantly increased in model group（P<0.05）. However, compared with model group, the liver pathological changes in each treatment group were significantly relieved, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression of TLR4 and NF-κB p65 in liver tissues were significantly reduced（P<0.05）.In addition, the changes of TLR4 and p-NF-κB p65 protein levels in liver tissue were consistent with the changes of TLR4 and NF-κB p65 mRNA. CONCLUSION Fuzilizhong decoction attenuates the inflammatory damages of NAFLD in rats by inhibiting TLR4/NF-κB p65 signaling pathway.     

p300/p53/Smad3 pathway involved in aging-related atrial fibrosis in human atrial fibroblasts

AIM To investigate the role of p300 in aging-related atrial fibrosis in human atrial fibroblasts （HAFs） and its potential mechanism. METHODS HAFs were obtained from human left atrial tissue, and the senescence model was established by cell passage. Senescence-associated β-galactosidase （SA-β-Gal） staining was used to detect the cell senescence, and Western blot was used to determine the protein levels of p300, p53, Smad3 and other senescence and fibrosis associated proteins in HAFs. RESULTS Compared to passage 3 HAFs, the proportion of senescent cells, and the protein levels of p300, p53, p-Smad3 and other senescence and fibrosis associated proteins were increased in HAFs at passage 7 and 11 （P<0.05）. After treated with curcumin （a p300 inhibitor） or transfection with p300 small-hairpin （sh） RNA plasmid, the protein levels of p300, and the senescence and fibrosis associated proteins were decreased in HAFs at passage 7（P<0.05）. Up-regulation of p300 by transfection with p300 over-expression plasmid increased the protein levels of p53, Smad3 and MMP-2 in HAFs at passage 3 （P<0.05）. CONCLUSION p300/p53/Smad3 signaling pathway plays an important role in aging-related atrial fibrosis in HAFs.     

MicroRNA-9-5p mediates sympathetic overactivity by targeting KCNN3 in paraventricular nucleus of rats with T2D

AIM To investigate whether microRNA-9-5p （miR-9-5p） mediates sympathetic overactivity by targeting KCNN3 （potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3） gene,which encoded small-conductance calcium-activated potassium channel 3 （SK3） protein, in paraventricular nucleus （PVN） of rats with type 2 diabetes mellitus （T2D）. METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus （rAAV）-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control （DC） group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus （DM） rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced （P<0.05）. However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.     

绵羊WNT5A基因克隆及其组织表达分析

WNT5A(Wnt family member 5A)参与了多种细胞的增殖、凋亡和分化等生物学过程,在乳腺形态发生、毛囊发育等方面发挥了重要作用.该试验利用RT-PCR获得绵羊WNT5A基因的CDS区,分析WNT5A蛋白的结构特征并利用RT-qPCR检测WNT5A基因在9个组织中的表达情况.绵羊WNT5A基因的CDS区...     

基于YOLOv5s-ESTC的肉苁蓉检测

为了解决因梭梭和红柳等宿主遮挡、样本分布密集、样本大小不均衡等造成人工种植肉苁蓉检测精度低以及模型参数量过大难以向嵌入式设备移植等问题,该研究提出一种基于改进YOLOv5s的人工种植肉苁蓉轻量化检测方法。首先,将YOLOv5s的主干网络替换为EfficientNetv2网络,以减少模型参数量和计算复杂度进而实现模型轻量化,便于后期将模型向嵌入式设备部署;其次,为了增强模型对小目标肉苁蓉特征信息的提取能力,将C3模块与Swin Transformer Block进行整合,得到C3STR模块,将其引入主干网络,并将其输出的特征图与Neck网络中的特征图进行融合;最后,在检测头前端与颈项加强网络之间添加CA注意力机制,以弱化背景信息、聚焦目标特征,提高网络检测性能。试验结果表明,该模型对于肉苁蓉的检测精度和准确率分别为89.8%和92.3%,模型的参数量和计算量分别为5.69×10⁶ MB和6.8 GB,权重文件大小为11.9 MB,单幅图像推理时间为8.9 ms,能够实现实时性检测。同其他主流模型相比,改进后的模型的检测精度分别比SSD、Faster R-CNN、YO...     

改进YOLOv5s对病理学图像中猪只小肠绒毛的检测

为解决传统小肠绒毛需要专业人员手动检测耗时耗力且存在主观性和不稳定性等问题,同时提高在复杂病理学图像中小肠绒毛检测的准确率和效率,该研究提出基于改进YOLOv5s检测复杂病理学图像下猪只小肠绒毛的方法。首先,采用串联形式的混合池化对空间金字塔进行优化,增强特征提取与特征表达,提升检测精度;然后引入一种基于注意力机制的网络模块(simpleattentionmechanism,SimAM)与Bottleneck中的残差连接相结合,使用SimAM对Bottleneck中的特征图进行加权,得到加权后的特征表示,利用注意力机制加强模型对目标的感知。试验结果表明,该研究算法的平均精度（average precision）和每秒传输帧数（frame per second,FPS）达到92.43%和40帧/s。改进后的YOLOv5s在召回率和平均精度上相较改进前提高2.49和4.62个百分点,在不增加模型参数量的情况下,每帧图片的推理时间缩短1.04 ms。与经典的目标检测算法SSD、Faster R-CNN、YOLOv6s、YOLOX相比,平均精度分别提高15.16、10.56、2.03和4.07...     

黄芪多糖在LPS诱导的DF-1细胞炎症反应中的抗炎作用及其调节机制

为探讨黄芪多糖(Astragalus polysaccharide,APS)在LPS(lipopolysaccharide,LPS)诱导的鸡胚成纤维细胞系DF-1(chicken embryonic fibroblast cell line,DF-1)炎症模型中对IL-1β和TNF-α表达的影响,以及细胞因子信号转导抑...     

东北典型农区土地利用变化的生态效应研究——以公主岭市为例

在人为活动占优势的景观内,不同土地利用方式和强度产生的生态效应是不同的,并且可以直观地反映在生态系统的结构和组成上。因此,本文利用Landsat TM和MSS遥感数据,以具有相对完整的自然生态过程的小流域作为评价单元,借助GIS空间分析和地统计分析功能,从景观生态学角度提出了景观生态指数来反映LUCC变化所带来的生态效应。研究发现,公主岭市1975—1986年间由于土地利用/土地覆被变化造成的生态风险不断增加,随后逐渐减小,到2000年局部地区又有所回升,这种变化与该地区土地利用类型的变化,特别是耕地、林地和草地的变化趋势呈高度相关,说明该地区土地利用/土地覆被变化对生物多样性及生态安全具有重要影响。     

农牧交错带不同封育时间对植物特征值及多样性的影响

为研究我国北方农牧交错带不同封育时间对干旱、半干旱草原植物特征值及多样性的影响,同时探索农牧交错带草原封育周期,本研究选取位于我国农牧交错带的宁夏盐池人工封育草原为研究对象,采用样方调查法,于每年7-8月份(植物生长季节)对封育区植被生长状况进行调查,调查内容包括:植物名称、植物种数、株数、盖度、高度、地上生物量(鲜重)等。运用生态学、地统计学原理,结合Biodiversity Pro、Microcal Origin等生态学统计软件对外业数据进行处理、分析,结果表明:封育2-3年后植物群落优势种优势地位得到巩固,而一些伴生种则在竞争中消失;封育区植物特征值在封育2-3年后达到最大,如地上生物量(鲜重)在封育3年达到最大值,为6907.75kg/hm2,此后呈下降趋势;封育区主要植物重要值及植物物种多样性均在封育2-3年后出现最大值,此后也不同程度地呈下降趋势。