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51.
52.
为了探讨大豆活性肽对仔猪生长性能、血液指标及氧化应激参数的影响,试验选取仔猪48头,随机分为2组,每组设3个重复,每重复8头。对照组饲喂基础日粮,试验组饲喂用大豆活性肽替代2%豆粕的日粮。结果:添加2%大豆活性肽,20 d时,显著提高了仔猪粗脂肪、粗蛋白的消化率(P<0.05);血清总蛋白(TP)、血清白蛋白(ALB)和碱性磷酸酶(ALP)水平略高于对照组;50 d时,大豆活性肽显著提高了仔猪干物质、粗灰分、粗脂肪、粗蛋白、粗纤维的消化率(P<0.05),而且总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性及TP水平显著高于对照组(P<0.05);全程试验组日增重均显著的高于对照组,料重比、腹泻率显著低于对照组(P<0.05),然而对尿素氮(BUN)和甘油三脂(TG)影响不明显。 相似文献
53.
本科酶工程课程教学实践与体会 总被引:1,自引:0,他引:1
酶工程是高校生物工程和生物技术专业重要的专业课。结合课程教学实践,总结了酶工程教学过程中的几点体会,以提高酶工程教学质量,达到学生综合能力的培养目的。 相似文献
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柳蛎盾蚧危害与丁香防御蛋白活力的关系 总被引:1,自引:0,他引:1
在柳蛎盾蚧的未危害期(5月末)、危害盛期(6月末)、危害弱期(7月末)、危害末期(8月末),测定高抗类[什锦丁香等7种(品种)]、中抗类[西南丁香等3种(品种)]、易感类[紫丁香等2种]、高感类(红丁香)13种(品种)丁香叶中的POD,SOD,CAT,PPO,PAL,TI和CI 7种防御蛋白的活性.结果表明:危害时期,丁香种(品种)对丁香防御蛋白活力有极显著影响(P<0.01).POD活性,在危害盛期,在高抗、中抗类丁香中均显著升高(P<0.05),而在易感、高感类中应激滞后,即分别在危害弱期和危害末期升高.CAT在易感类和高感类丁香防御上起主导作用,其活性在危害盛期显著升高,而中抗和高抗类未见明显变化.SOD活性,在危害盛期高抗类丁香中无显著变化,而其余3类则显著降低.PPO活性在未危害期的高低与抗性相关,在虫害盛期诱导表达的强弱也与抗性相关;PAL活性未能反映抗性品种间的差异.TI和CI活性,在未危害期的高低与其抗虫性无明显相关性,在危害盛期的升高幅度与抗虫性呈正相关.综上所述,PPO在未危害期的活力可作为筛选丁香抗虫种(品种)的指标,危害盛期POD,PPO,TI和CI活性升高的幅度与丁香抗虫性呈正相关. 相似文献
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Abbas Rajaeerad Gholam R. Ghorbani Mohammad Khorvash Ali Sadeghi-Sefidmazgi Amir H. Mahdavi Mirja R. Wilkens 《Journal of animal physiology and animal nutrition》2021,105(1):14-25
This study investigated the effects of low potassium diets with different levels of Ca compared to two diets low in dietary cation–anion difference (DCAD) fed prepartum as a strategy to prevent hypocalcemia on sorting behaviour, total tract digestibility, oxidative status and energy and protein metabolism of transition cows. Forty-eight pregnant dairy cows were assigned to 4 treatment groups: Low Ca, low K (LCLK), High Ca, low K (HCLK), Supplementation with anionic mineral mixture (AMS) supplementation with SoyChlor (CAS). After parturition, all animals were fed a standard postpartum diet. Data were collected until 21 DIM. Prepartum urinary pH was significantly reduced by the low DCAD diets, while postpartum Ca homeostasis was affected by the HCLK ration. Feeding AMS induced sorting against particles <1.18 mm in favour of particles >19 mm prepartum. In contrast, cows fed CAS showed an increase in selective consumption of fine particles and sorted against longer particles similar to the HCLK and LCLK groups. Postpartum sorting activity was not affected by the dietary treatments. After calving, apparent digestibility of NDF was significantly reduced in the HCLK group. Prepartum, we observed effects on serum concentrations of non-esterified fatty acids were higher and insulin sensitivity was lower in the AMS group. Blood urea nitrogen (BUN) was decreased in cows fed the CAS ration. Postpartum, we found serum protein to be decreased with the low DCAD diets while BUN was decreased in the CAS group. The low DCAD rations increased prepartum serum malondialdehyde concentrations, while postpartum total antioxidant capacity was lower in the HCLK and the AMS group. From these data, we conclude that AMS decreased prepartum intake due to compromised palatability. Intermediate protein metabolism was affected by the low DCAD diets, while parameters of oxidative stress were probably affected by acid–base balance and Ca homeostasis. 相似文献
57.
Md Shafiqul ISLAM Urara SHINYA Mitsuhiro TAKAGI Takao AKAHOSHI Akira YABUKI Shahnaj PERVIN Tofazzal Md RAKIB Mohammad Mahbubur RAHMAN Martia Rani TACHARINA Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):254
Bovine isoleucyl-tRNA synthetase (IARS) disorder, a major cause of weak calf syndrome, is caused by a homozygous missense (c.235G>C) mutation in the bovine IARS gene of Japanese Black (JB) cattle, which was identified in 2013. However, the extent to which the carrier rate has changed at Kagoshima prefecture, Japan, and whether the carrier status is associated with any clinical or reproductive problems, have yet to be ascertained. In this study, using a real-time polymerase chain reaction-based genotyping assay, we determined the carrier rate in a regional JB cow population at Kagoshima prefecture. Comparative analyses were performed on the metabolic profile test (MPT) results and reproductive performance data obtained for heterozygous carrier and homozygous wild-type cows. In 2009 and 2018, DNA samples were collected from 130 and 462 clinically healthy JB cows, respectively, in Kagoshima prefecture. MPT results and reproductive performance data were evaluated for 62 cows, comprising four heterozygous carriers and 58 wild-type cows. Genotyping revealed that the carrier rate was 6.9% in 2009 and 1.5% in 2018, the difference of which was statistically significant (P<0.005). There were no statistically significant differences between the carrier and wild-type cows with respect to either MPT results or reproductive performance, indicating that the carrier cows have necessary IARS activity to maintain minimal health and reproductive potential. 相似文献
58.
Yueting Pan Yongjia Wang Shanning Lou Metha Wanapat Zhaofeng Wang Wanhe Zhu Fujiang Hou 《Journal of animal physiology and animal nutrition》2021,105(4):611-620
Low selenium (Se) in soil and forage can adversely affect on the quality of animal-derived foods, and hence on human health. Lambs grazed on mixed pastures of alfalfa (Medicago sativa) and tall fescue (Festuca arundinacea) were supplemented with five levels of Se [0, 3, 6, 9 and 12 µg/kg body weight (BW)]. The intake of dry matter (DM) and organic matter (OM) varied with the level of Se supplementation, with a peak at 6 µg Se per kg BW (p ≤ 0.05). Gross energy (GE) intake, digestive energy (DE) intake and metabolic energy (ME) intake were higher at 6 µg Se per kg BW than at other Se levels (p < 0.01); in addition, methane energy (CH4-E) output was lower at 6 µg Se per kg BW. Supplementation with Se significantly increased nitrogen (N) intake, faecal N and urine N, for which the peak values were 20.2 g N/, 5.62 g N/day and 7.92 g N/day, respectively, at 6 µg Se per kg BW. Se intake, blood Se, faecal Se, urine Se and retained Se were negatively correlated with forage crude protein (CP) content (p < 0.001) but were positively correlated with the content of neutral detergent fibre (NDF) (p < 0.001) and acid detergent fibre (ADF) (p < 0.001). Thus, we recommend the addition of 6 µg Se per kg BW to sheep grazed on pastures in regions with low soil Se. 相似文献
59.
Bianca Ferreira Olivieri Camila Urbano Braz Fernando Brito Lopes Elisa Peripolli Rafael Medeiros de Oliveira Silva Rosana Ruegger Pereira da Silva Corte Lucia Galvo de Albuquerque Anglica Simone Cravo Pereira Nedenia Bonvino Stafuzza Fernando Baldi 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2021,138(1):80-90
The aim of this study was to identify differentially expressed genes (DEG) in the Longissimus thoracis muscle of Nelore cattle related to fatty acid (FA) profile through RNA sequencing and principal component analysis (PCA). Two groups of 10 animals each were selected containing PC1 and PC2 extreme DEG values (HIGH × LOW) for each FA group. The intramuscular fat (IMF) was compared between cluster groups by ANOVA, and only the sum of monounsaturated FA (MUFA) and ω3 showed significant differences (p < .05). Interestingly, the highest percentage (95%) of phenotypic variation explained by the sum of the first two PC was observed for ω3, which also displayed the lowest number of DEG (n = 1). The lowest percentage (59%) was observed for MUFA, which also revealed the largest number of DEG (n = 66). Since only MUFA and ω3 exhibited significant differences between cluster groups, we can conclude that the differences observed for the remaining groups are not due to the percentage of IMF. Several genes that have been previously associated with meat quality and FA traits were identified as DEG in this study. The functional analysis revealed one KEGG pathway and eight GO terms as significant (p < .05), in which we highlighted the purine metabolism, glycolytic process, adenosine triphosphate binding and bone development. These results strongly contribute to the knowledge of the biological mechanisms involved in meat FA profile of Nelore cattle. 相似文献
60.