Effect of biologically active substances present in water extracts of white mustard and coriander on antioxidant status and lipid peroxidation of mouse C2C12 skeletal muscle cells

Coriander and white mustard, an annual plants originated in the Mediterranean region, have been cultivated and used as spices for a long time. Recent studies have shown that they may constitute a potential source of phenolic compounds. The aim of this study was to evaluate the content of polyphenols in coriander and white mustard water extracts and to investigate their antioxidant activity in C2C12 mouse skeletal muscle cells, which serve as a good model of cells with intensive metabolism. HPLC analysis showed that polyphenols were able to permeate from the water extracts of studied plants into the undifferentiated myoblasts as well as myocytes undergoing differentiation, increasing the concentration of reduced glutathione and upregulating glutathione reductase and peroxidase activity. White mustard and coriander extracts also decreased the levels of oxysterols and sum of tiobarbituric acid reactive substances (TBARS) in both: myoblasts and differentiating myocytes, demonstrating protective effect on cell membranes. The obtained results indicate that polyphenols synthesized by both herbs may have beneficial effects on muscle tissue.     

Comparison of sampling sites and detection methods for Haemophilus parasuis

Objective To improve the isolation rate and identification procedures for Haemophilus parasuis from pig tissues. Design Thirteen sampling sites and up to three methods were used to confirm the presence of H. parasuis in pigs after experimental challenge. Procedure Colostrum‐deprived, naturally farrowed pigs were challenged intratracheally with H parasuis serovar 12 or 4. Samples taken during necropsy were either inoculated onto culture plates, processed directly for PCR or enriched prior to being processed for PCR. The recovery of H parasuis from different sampling sites and using different sampling methods was compared for each serovar. Results H parasuis was recovered from several sample sites for all serovar 12 challenged pigs, while the trachea was the only positive site for all pigs following serovar 4 challenge. The method of solid medium culture of swabs, and confirmation of the identity of cultured bacteria by PCR, resulted in 38% and 14% more positive results on a site basis for serovars 12 and 4, retrospectively, than direct PCR on the swabs. This difference was significant in the serovar 12 challenge. Conclusion Conventional culture proved to be more effective in detecting H parasuis than direct PCR or PCR on enrichment broths. For subacute (serovar 4) infections, the most successful sites for culture or direct PCR were pleural fluid, peritoneal fibrin and fluid, lung and pericardial fluid. For acute (serovar 12) infections, the best sites were lung, heart blood, affected joints and brain. The methodologies and key sampling sites identified in this study will enable improved isolation of H parasuis and aid the diagnosis of Glässer's disease.     

Characterization,phylogenetic analyses and pathogenicity of Bipolaris setariae associated with brown stripe disease on sugarcane in Yunnan,China

Brown stripe disease is a severe foliar fungal disease of sugarcane worldwide and is widespread in all sugarcane planting areas in China. Brown stripe is a major disease that seriously affects the output and quality of the sugarcane industry in Yunnan Province, China's second-largest sugar base, while the pathogen of this disease remains not yet fully understood. To address this, we isolated and identified the fungi associated with 68 leaf samples showing typical symptoms of brown stripe from 22 sugarcane varieties in different areas of Yunnan Province. A total of 113 isolates were obtained, which were morphologically similar. Of these, 64 representative isolates were sequenced for the internal transcribed spacer region (ITS), GAPDH and EF-1α loci. All representative isolates grouped with the type strain of Bipolaris setariae in the phylogenetic trees inferred with individual and concatenated sequences of ITS, GAPDH and EF1-α. Pathogenicity test results showed that B. setariae strains were able to induce typical symptoms of brown stripe. The results obtained in this study clarify that only B. setariae is associated with sugarcane brown stripe in Yunnan, China. It is recorded here for the first time as a pathogen causing sugarcane brown stripe in Yunnan, and it is able to infect many major cultivars and new varieties, posing a new threat to the sugar industry in Yunnan Province. In addition, these results provide the scientific basis for the future breeding of disease-resistant varieties and effective prevention and control of sugarcane brown stripe disease.     

Research on Dynamics of Installation Way for Active Isolation Actuator on Flexible Foundations

The dynamic characteristic of active isolation atuator on flexible foundations was studied under the installation of parallel structure and foundation cancellation.The influencing relationships between active isolation force of the different installation ways and passive isolation system and flexible foundation were analyzed and compared.Scientific basis for selecting installation ways of active isolator is provided in this paper.     

对橡胶悬架装置动特性的改进探讨

以橡胶悬架装置为研究对象,考虑其动态特性与高速车辆动力总成低频与高频要求不同之间的矛盾,试图通过改进橡胶悬架装置的结构提高其动特性。建立了改进结构的橡胶悬架装置的力学模型,并对其进行了理论分析和仿真计算,结果表明改进后的橡胶悬架装置的动特性有了显著提高,对我国橡胶悬架装置的结构改进具有借鉴和指导意义。     

大理州“红阳”猕猴桃真菌性枝干的发生与病原鉴定

以大理州"红阳"猕猴桃枝干为试材,采用田间调查法调查病原菌对其危害状况,对采集的10份病样采用常规组织分离法分离病原,单孢纯化法获得病原菌,采用形态和分子法鉴定病原种类,以期明确大理州"红阳"猕猴桃枝干病害的发生状况及危害病原。结果表明:大理州"红阳"猕猴桃枝干病害发生严重,病株率高达69.6%,5个采样点共分离得到15个菌株,其中以链格孢菌(Alternaria alternata)、厚坦镰刀菌(Fusarium chlamydosporum)、小新壳梭孢菌(Neofusicoccum parvum)和间座壳属中一种(Diaporthe bohemiae)共4类病原菌出现频率最高,是优势种,分离率分别为55.6%、52.4%、49.8%和50.1%。利用柯赫氏法则对分离所得的所有菌株进行致病性测定,证实了优势菌株对猕猴桃茎杆的致病性,进一步明确了引起大理州猕猴桃茎杆的病原真菌。     

菜籽中2—羟基—3—丁烯基硫代葡萄糖苷的分离与纯化制备

本文报道了用弱离了交换剂DEAE Sephadex A-25作固定相,K_2SO_4溶液作流动相,采用自动柱层析仪分离制备硫代葡萄糖苷结晶粗品,然后采用制备HPLC,Φ8×250 mm柱,充填Hitachi 3050(ODS),5×10~(-2)乙腈作流动相,进一步分离纯化,制得2-羟基-3-丁烯基硫代葡萄糖苷的钾盐晶体,以质谱(MS),红外图谱(IR),紫外图谱(UV)和HPLC图谱证实,结晶物纯度为色谱纯。     

高纯度茶氨酸分离制备工艺研究

本文研究一种从提取茶多酚后的残留液中提纯茶氨酸的制备工艺;首先通过ZTC—型天然澄清剂对茶多酚生产废液絮凝处理,随后应用一种特制的弱极性大孔树脂(JAD-1000)对处理液进行初步分离,制备含量在50%以上的茶氨酸粗品,再通过C18层析柱,按照正交实验技术筛选的分离纯化工艺参数,对茶氨酸粗品溶液进行分离纯化制备高纯度茶氨酸;结果表明用pH值3.0的水按照60ml/min的流速进行洗脱,收集11.4min-13.5min段的洗脱馏份,浓缩冻干,检测知茶氨酸的回收率为78.4%,茶氨酸含量达98.2%。     

减毒鸡沙门菌疫苗株97A的免疫保护效力试验

减毒沙门菌疫苗株97A免疫鸡经鸡沙门菌强毒株Sg9攻击后,对免疫攻毒组和攻毒对照组鸡进行病理学检查和细菌分离,以进一步评价97A疫苗的免疫保护效力。结果表明,免疫攻毒组鸡肝脏、肺脏和肾脏等实质器官仅有轻到中度的炎症反应,而攻毒对照组鸡则表现出了严重的组织病理学变化,同时减毒鸡沙门菌疫苗株97A能显著地限制强毒株在鸡体内的定居和增殖,显示出了良好的免疫保护效力。     

哺乳动物上皮细胞抗菌肽分离纯化技术研究进展

抗菌肽是在动植物体内发现的氨基酸数目少于100个的一类多肽,是哺乳动物防御体系的一个重要组成部分,具有分子质量小、对热稳定、水溶性好、广谱抗菌和作用机制独特等特点。文章主要综述了几种哺乳动物上皮细胞抗菌肽的分离纯化技术研究概况。