亚成体东北虎感染巴氏杆菌死亡一例

2009年1月11日,北方森林动物园发生一起自繁自养的亚成体东北虎感染巴氏杆菌死亡病例。本病例从发病到死亡共5 d时间,病程较短,以食欲下降至废绝,饮水量不变,精神沉郁,口腔腺体分泌增多、流涎,呈滴水样,腹部疼痛感明显为特征,口服诺氟沙星和多酶片治疗无效后改用肌注头孢哌酮钠和庆大霉素治疗,3 d后出现神经症状抽搐死亡。剖检肝脏表面有大量的针尖大小黄白色点状坏死灶。根据临床症状、病理剖检和实验室检查,确诊为巴氏杆菌感染。     

Corynebacterium pyrogenes; a biochemical and serological study

The study comprises 136 strains of Corynebacterium pyogenes originating from cattle (105), swine (20), sheep (1), and insects (10). For comparison 2 strains of human origin and 1 strain of Gorynebacterium hemolyticum were examined.One of the bovine strains was atypical, being gelatinase-negative, otherwise the strains of Cb. pyogenes were found to be biochemically identical apart from minor deviations in fermentation patterns (Table 1). Neither were antigenic differences demonstrated (gel diffusion analyses, Figs. 1 and 2).Both of the human strains agreed biochemically with Cb. pyogenes (Table 1). By gel diffusion cross analyses one of them was found to be identical with Cb. pyogenes, the other not, though anti-genically related to it (Fig. 2).Gb. hemolyticum deviated biochemically as well as serologically from Gb. pyogenes, but the 2 organisms shared antigenic determinants (Fig. 3).     

乳酸菌抗幽门螺旋杆菌感染的研究进展

综述了近年来对乳酸菌抗幽门螺旋杆菌感染的研究进展,内容包括乳酸菌抗幽门螺旋杆菌感染的机制及应用、乳酸菌抗原递送载体、乳酸菌疫苗等。     

意蜂来源的微孢子虫对不同日龄中蜂成年工蜂的侵染性

为探明意蜂来源的微孢子虫对不同日龄中蜂成年工蜂的交叉侵染性,将纯化自意蜂的微孢子虫分别接种无微孢子虫感染的2日、5日、8日、11日、14日龄的中蜂工蜂,当每只蜂经口接种5.12×104个孢子,在30±1℃,45%～60%RH,黑暗环境中饲养12天后,2～14日龄的中蜂工蜂的感染率为46.7%～72.6%,平均感染率为65.8%;其中2日龄的感染率显著(P<0.05)低于5～14日龄的感染率。     

中蜂来源的微孢子虫对意蜂工蜂的侵染性研究

为探讨中蜂来源的微孢子虫对意蜂的侵染性,本试验将纯化自中蜂的微孢子虫,以每只蜂1×105个和5×105个孢子的接种量,分别给2日、5日、8日、11日龄无微孢子虫感染的意蜂成年工蜂接种,在30℃±0.5℃,65%～80%RH,黑暗中隔离饲养10日后,各日龄工蜂的平均感染率达86.3%～100%,每只蜂中肠的平均产孢量为5.60×105～3.39×106个。在两接种量下,2日、5日龄意蜂工蜂的产孢量均显著(P<0.05)高于8日、11日龄的产孢量。试验首次证实了中蜂来源的微孢子虫对意蜂成年工蜂具较强的侵染性。     

实验性犬细小病毒感染及其病理形态学研究

取2～3月龄大13只(10只肠道驱虫),禁食24h后,分组分别经口鼻、静脉、肌肉接种TCID_(50)/ml为4×10~(5·75)的犬细小病毒(CPV)第四代猫肾细胞培养毒11.5或25.0m1/kg,再禁食48h。结果,各犬均于接种后第4d起陆续典型发病,粪便HA试验阳性,负染见CPV粒子。主要病理学变化是,小肠出血性坏死性炎,心肌灶状出血和颗粒变性;胸腺皮质的胸腺细胞及淋巴结皮质的淋巴细胞死亡、减少;在一些小肠隐窝上皮细胞、淋巴结的淋巴细胞和网状细胞以及胸腺的胸腺细胞和上皮性网状细胞核内见嗜酸性、嗜硷性或两染性包涵体;3只3月龄犬膈肌灶状出血。小肠粘膜扫描电镜观察,粘液增多,绒毛肿胀。其上的横沟变钱或消失。杯状细胞开口扩张,数目增加。电镜观察,在小肠隐窝上皮未分化细胞的核内见直径为27nm的CPV粒子,并对其形态发生作了描述。     

用绵羊进行性肺炎病毒实验感染山羊的研究

用绵羊进行肺炎病毒接种４只山羊３－４个月后，可观察到接毒山羊都出现了发育迟缓和消瘦现象，并有１只山羊山现了明显的临床病症。而未接毒的对照山羊则发育正常。接毒２８ｄ后，可以从接毒山羊的外周血单核细胞中分离到病毒。病理剖检发现４只接毒山羊中有１只山羊的多种器官出现了较为严重的病变，组织学检查则可看到典型的间质性肺炎、中度的脑炎和较为严重的脾炎的症变。以上的结果表明ＯＰＰＶ可以感染山羊，并对山羊有较强的     

Prevalence of antibodies to Toxoplasma gondii in cats, dogs and horses in Sweden

Samples of serum or plasma taken during 1986 and 1987 from 244 pet cats, 303 dogs and 219 horses, randomly selected among animals referred to the Animal Clinics of the Swedish University of Agricultural Sciences, were screened by enzyme-linked immunosorbent assay (ELISA) for antibodies to Toxoplasma gondii. 42% of cats, 23% of dogs and 1% of horses examined were found seropositive.