Heme oxygenase-1 alleviates alcoholic liver steatosis: histopathological study

Excessive alcohol consumption is one of the most important causes of hepatic steatosis, which involves oxidative stress. In particular, increased oxidative stress has been strongly linked to stimulation of the expression of heme oxygenase-1 (HO-1). This study aimed to investigate whether HO-1 could alleviates alcoholic steatosis in rats. Male Wistar rats were randomly divided into 4 groups: 1) the control group, 2) the EtOH group, 3) the EtOH + ZnPP-IX group and 4) the EtOH + Hemin group. Liver histopathology was investigated in weeks 1 and 4 after the start of the treatment period. Alcohol treatment significantly increased the hepatic malondialdehyde (MDA) levels, an oxidative stress marker. In addition, it increased the triglyceride, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in both weeks. Gross examination demonstrated a yellowish and slightly enlarged liver in the alcohol-treated rats. Hematoxylin and eosin (H&E) and Oil Red O staining indicated hepatic steatosis, which was characterized by diffuse, extensive fatty accumulation and discrete lipid droplets of variable size in hepatocytes of the alcohol-treated rats. Administration of the HO-1 inducer hemin resulted in upregulation of hepatic HO-1 gene expression, reduced the MDA, triglyceride, ALT and AST levels and alleviated alcoholic hepatic steatosis, whereas administration of the HO-1 inhibitor zinc protoporphyrin IX (ZnPP-IX) resulted in downregulation of hepatic HO-1 gene expression and could not alleviate alcoholic hepatic steatosis either week. In conclusion, HO-1 could alleviate alcoholic hepatic steatosis in male Wistar rats and may be useful in development of a new therapeutic approach.     

Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig

This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to predicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment included a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48% of similar nucleotide sequence with that of Bos,Pan troglodytes,Homos,Macaca,Rattus,Mus,Gallus and Danio retio,respectively.The protein structure analysis results showed that the protein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 16 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly conserved in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.     

涝胁迫对豫楸1号4种砧木嫁接苗渗透调节物质的影响

通过对涝渍胁迫条件下豫楸1号4种砧木嫁接苗渗透调节物质（Pro、可溶性糖、可溶性蛋白）活性的测定,结果表明：在持续涝渍胁迫下,3种渗透调节物质的变化趋势基本一致,整体变化趋势为先上升后下降,只是峰值出现的时间有差异。     

长杂2号在襄垣县的表现与高产栽培技术

对优质、高产谷子杂交种长杂2号的特征特性及其在襄垣地区的表现进行了分析,结果表明,该杂交种丰产、稳产、抗逆性强。针对其特点提出了相应的优质高产栽培措施:适时播种、适时喷除草剂并间苗、公顷留苗不应超过30万株、综合防治病虫害、及时抢收,为该品种大面积种植提供技术指导。     

基于STM32F407的双CAN总线设计与实现

本文是基于意法半导体(ST)新推出的一款高性能Cortex TM-M4内核的ARM芯片STM32F407ZGT6,进行的双CAN总线设计。在开发过程中采用了ST提供的可视化图形界面开发工具STM32Cube进行底层驱动的配置,简化了设计工作。但由于该工具链接的固件库函数存在传递参数错误,使得CAN总线无法接收数据,本文对该库函数进行了更正。     

蜂王浆对尾静脉注射4T1细胞小鼠抗氧化能力的影响

探讨蜂王浆对尾静脉注射4T1细胞小鼠的抗氧化和免疫力的影响.将4T1细胞或其与蜂王浆的混合物通过尾静脉注射入小鼠体内,ELISA法检测蜂王浆对小鼠血清、肝脏和肾脏中与抗氧化能力相关的指标及血清中和免疫力相关细胞因子水平的影响.结果表明,和模型对照组相比,蜂王浆(50、100 mg·mL~(-1))可以显著提高小鼠血清内T-AOC、IFN-α(P0.05)水平,降低IL-4的水平(P0.05);蜂王浆剂量为50 mg·m L~(-1)时,IFN-γ的含量显著增加(P0.05).蜂王浆(100 mg·mL~(-1))显著提高肾脏中T-AOC的水平(P0.05).可见,蜂王浆可以改善尾静脉注射4T1细胞小鼠的抗氧化能力和免疫力.     

不同产量潜力甘薯光合产物的生产、运转与块根产量的关系

选取中国主栽的食用型甘薯品种龙薯9号、红香蕉、泰中6号、苏薯8号、遗字138和北京553进行大田试验,研究不同产量潜力甘薯品种光合产物的生产、运转及其与块根产量的关系。结果表明,高产品种整个生育期干物质积累快,光合产物向块根输送多,同化物在块根中的分配率较高,而且高产品种的标记叶合成的光合产物能够较多地运出。因此,叶片光合作用形成的光合产物能够及时地往外运输,是高产甘薯品种高产的重要保证。高产品种具有较高的经济系数。     

两系杂交油菜早中熟组合精量直播栽培性状比较研究

开展优质核不育两系杂交油菜早中熟新组合比较试验,结果表明:2种熟期类型杂交组合间产量差异明显,中熟组合单位面积产量极显著高于早熟组合,对比分析产量构成因子,主要是平均角粒数多和千粒重重,2种熟期类型组合间株高和一次分枝数差异较小,油菜菌核病感病程度中熟组合相对较轻。因此,油菜生产不可一味选择和使用早熟类型品种或组合,不然将较难获得理想产量和生产效益。     

半夏茎腐病原菌对西和半夏幼苗叶片生理特性的影响

[目的]探讨半夏茎腐病原菌对半夏产生侵害的生理学机制。[方法]采用实验室培养试验研究了不同浓度的半夏茎腐病原菌孢子对半夏幼苗叶片生理特性的影响。[结果]随着病菌孢子浓度的增加,叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性先上升后下降,游离脯氨酸、可溶性糖和丙二醛(MDA)含量呈升高趋势,且与对照相比差异显著。[结论]试验结果为预防和治理半夏茎腐病提供了理论依据。     

核磁共振法测定甲哌鎓原药的含量

采用定量核磁共振法建立了甲哌鎓原药的定量方法。以重水为溶剂,仲丁基脲为内标物,以化学位移分别在δ3.12的甲哌鎓甲基和δ1.10的仲丁基脲甲基的1H-NMR信号作为定量峰。以甲哌鎓与仲丁基脲定量峰的峰面积比(AS/AI)对甲哌鎓与仲丁基脲的质量比(WS/WI)进行线性回归,线性相关系数为0.999 9;重复测定同一批次原药含量,变异系数为0.28%。采用核磁共振波谱法对甲哌鎓进行定量分析,操作简便,测定速度快,准确度高。