旋毛虫肌幼虫总RNA提取方法研究初报

【研究目的】探索高质量的旋毛虫(Trichinella spiralis)肌幼虫总RNA提取方法；【方法】利用Trizol试剂采用三种方法提取旋毛虫肌幼虫总RNA。(1)液氮预冷的研钵中研磨新鲜虫体至样品快融化时，加入适量的Trizol，继续研磨至液状后转移到离心管中；(2)A将新分离的虫体放到-20℃预冷的研钵中，在研磨过程中不断地添加液氮以保持虫体冷冻，之后对冷冻状的样品粉末继续操作；B把液氮冻存的新鲜旋毛虫肌幼虫取出，放到-20℃预冷的研钵中重复A的操作；(3)新鲜虫体放入匀浆器中，加适量Trizol，在冰水混合物中研磨20分钟。虫体破碎后按Trizol说明书继续抽提。最后通过凝胶电泳和紫外吸收光度值检测。【结果】用液氮和Trizol结合的方法，使用新鲜样品提取旋毛虫肌幼虫总RNA，在纯度和得率上都较为理想；【结论】使用新鲜的样品，且液氮与裂解液结合，是获得高质量RNA的可行方法。     

枣步曲幼虫空间分布型及抽样技术的研究

通过ＩｗａｏＸ－Ｘ回归测定，枣步曲幼虫的空间分布为奈曼－Ａ型分布或波松－二项分布，分布的基本成分为致密的个体群，个体群间及个体群内的分布均为随机分布，个体群的面积小于或等于１株树，对于估值抽样提出了两种方案，即整体抽样和层抽样，前者以随机抽样和棋盘式抽样为优，并给出了理论抽样公式，后者以最下层抽样代替整株抽样估计种群密度，可以提高抽样效率，降低抽样费用，且估计精度能达到科研与生产的要求，最后给出Ｉ     

鞭角华扁叶蜂滞育期幼虫空间分布型及抽样技术研究

鞭角华扁叶蜂滞育期幼虫空间分布型及抽样技术研究＊郑永祥崔相富吴道圣杨胜利陈绘画关键词鞭角华扁叶蜂、滞育期幼虫、空间分布、抽样技术鞭角华扁叶蜂［Ｃｈｉｎｏｌｙｄａｆｌａｇｅｌｉｃｏｒｎｉｓ（Ｆ．Ｓｍｉｔｈ）］属膜翅目（Ｈｙｍｅｎｏｐｔｅｒａ）、扁叶蜂科...     

辽宁三种松毛虫越冬幼虫过冷却点的研究

松毛虫越冬幼虫在越冬期间的存活率,除了取决于种的生物学特性而外,还取决于低温的强度、出现的时间、持续时间的长短等。冬季的最低温度不仅是决定三种松毛虫分布地区的重要原因,而且左右着松毛虫数量的变化。决定着冬眠幼虫能否存活和存活多少。尤其是越冬开始及越冬结束时,气候变化,影响最为显著。     

Identification and characterization of peptides binding AgEG1 from a phage display library

Endoglucanases are the main cellulolytic enzymes digestion as well as its good kinetic properties make it an attractive of Anoplophora glabripennis. Their high activities in cellulose target for development of cellulase inhibitors. In this study, random pepfide phage display technology was employed to identify peptides that bound the AgEG1, a member of endoglucanase isozymes. Phage clones with peptide LPPNPTK and XPP （X is residue T, L, A or H） motif frequently occurred in the selected phage population and showed a higher phage recovery than other clones. Peptide LPPNPTK was chemically synthesized and characterized tor its binding activities to AgEG1. The synthetic peptide exhibited high specificity for AgEG1. The peptide LPPNPTK has the potential to be developed into inhibitors of the endoglucanase of A. glabripennis.     

烟田烟青虫和棉铃虫幼虫体色变化及遗传规律初步研究

通过田间调查和室内饲养，将烟田混合发生的烟青虫和棉铃虫幼虫体色变化归纳为７种基本类型，其中在黄绿色型中烟表虫数量较多，占６３．９％；在绿褐色型中棉铃虫占９３．４％其它几种体色类型的幼虫均为棉铃虫。     

休眠松材线虫幼虫快速检疫方法

较系统地介绍了采用物理与化学相结合的方法,快速破除木包装中松材线虫幼虫休眠期,加速休眠幼虫转变为成虫,促进休眠幼虫的分离数量,缩短检疫时间,提高松材线虫的检出率。     

Environmental factors influencing larval sprat Sprattus sprattus feeding during spawning time in the Baltic Sea

The management of Baltic sprat is challenged by highly variable recruitment success and hence large stock fluctuations. Recent studies have identified the larval and early juvenile life stages to be critical for the survival rate of a sprat year class. Although prey abundance was found to be linked to larval survival success, an analysis identifying the functional relationship and relative importance of other environmental factors is still missing. Sprat larval feeding was investigated in 2002 during three cruises, covering the main spawning time in the Bornholm Basin, Baltic Sea. The aim of the study was to identify the key environmental factors determining the feeding success of larval sprat taking their potential interactions explicitly into account. An extension of generalized additive models (GAMs) was adopted that allows the inclusion of interaction terms in a non‐parametric regression model. The final model of sprat larval feeding success explained ～80% of the variance in the data and was based on the following environmental factors: bottom depth, cubed wind speed as proxy for small‐scale turbulence rates, degree of cloudiness as proxy for light conditions and prey density in combination with a feeding period–cloudiness interaction term. Our study demonstrates that the feeding success of sprat larvae in the Baltic Sea is controlled by a number of simultaneously acting key environmental factors.     

裂臀瓢虫属3种幼虫的形态记述（鞘翅目：瓢虫科）

简述了裂臀瓢虫属Ｈｅｎｏｓｅｐｉｌａｃｈｎａ Ｌｉ的研究情况,编制了该属中国已知幼虫种类检索表,首次描述了该属３种幼虫形态,幼虫标本保存于南京农业大学昆虫标本室。