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111.
AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs.  相似文献   
112.
LI Shu-guo  ZENG Qiu-tang 《园艺学报》2004,20(12):2232-2235
AIM: This study was designed to investigate the secretion of VEGF and its receptor (flt-1 or flk-1/KDR) protein by cultured bovine thoracic aortic endothelial cells treated with various insulin concentrations. METHODS: Endothelial cells was isolated from bovine thoracic aorta, and cultured in serum-free medium, then incubated with different insulin concentrations (30 mU/L, 300 mU/L, 3 000 mU/L). The level of VEGF and its receptor (flt-1 or flk-1/KDR) protein were detected by immunohistochemical staining. RESULTS: As compared with no insulin group, the expression of VEGF protein in low insulin concentration (30 mU/L and 300 mU/L) groups were significantly increased (P<0.01). The expression of VEGF protein in high insulin concentration (3 000 mU/L) group was significantly decreased (P<0.05). Howerer, no difference of the expression of VEGF receptor (flt-1 or flk-1/KDR) protein among all groups (P>0.05) was observed. CONCLUSION: Low concentration insulin up-regulates the VEGF protein expression while high concentration insulin down-regulates the VEGF protein expression in bovine thoracic aortic endothelial cells, but insulin had no directly effect on the VEGF receptor (flt-1 or flk-1/KDR) protein expression in bovine thoracic aortic endothelial cells.  相似文献   
113.
AIM: To study the expression of vascular endothelial growth factor (VEGF) in inflammatory mucosa of lacrimal sac. METHODS: Immunohistochemical S-P method was used to examine the expression of VEGF in the mocusa from 12 patients with chronic dacryocystitis and 8 volunteers. RESULTS: The positive rates of VEGF expression in different parts of the mocusa were: basal lamina: 44.3%±7.6%; surface epithelium: 16.9%±4.6%; connective tissue: 15.2%±4.9%, all normal mocusa of 8 cases were negative. There was a significant difference between the two groups (P<0.01), a significant difference among each part of the chronic inflammatory mocusa of lacrimal sac. CONCLUSION: VEGF may play an important role in hyperplasia of inflammatory mucosa of lacrimal sac.  相似文献   
114.
AIM: To explore a new method of hepatocyte growth factor (HGF) inducing bone marrow mesenchymal stem cells (MSC) to differentiate into cardiomyocytes. METHODS: Bone marrow MSC was cultured with DMEM media (10% fetal calf serum) 4-6 passages, and induced by HGF (10 μg/L) for 30 d. Automatical beating of the differentiated cells was observed daily with transverse microscopy, or under condition of 0.1% isoproterenol or cal-cium-deprived incubation. Specific cardiac myosin in the cells was indentified by immunochemistry. RESULTS: At 14-20 d of differentiation, bone marrow mesenchymal stem cells formed clones, in 10%-50% of which spontaneous beating cell-mass had come to continuously exist. Isoproterenol increased the beating rate and calcium-deprived media inhibited the beating. The cells were identified to be cardiomyocytes by expression of cardiac myosin heavy chain. CONCLUSION: HGF may induce bone marrow mesenchymal stem cells into cardiomyocytes with high efficiency, but the differentiating pathway of stem cells remains to be further studied.  相似文献   
115.
AIM: To investigate the role of adrenomedullin (AM) in diabetic nephropathy. METHODS: We observed the changes in the expression and secretion of AM, TGF-β1 in the cultured human mesangial cells under high glucose condition and the contents of the laminin and type IV collagen in the supernatants. The effect of intervention with AM was also observed. RESULTS: High glucose condition resulted in increase in the expression and secretion of AM、 TGF-β1、 laminin and type IV collagen. AM reversed the influence of high glucose on the cultured human mesangial cells. CONCLUSION: These results showed that high glucose condition is one of stimulating factors of AM and the renal protective action of AM may be associated with suppression of TGF-β1 and reducing excessive accumulat ion of laminin and type IV collagen.  相似文献   
116.
AIM: The effects of YIGU capsule on proliferation and IGF-I mRNA protein expressions in osteoblasts were studied. METHODS: (1) Forty 12-month old Sprague-Dawley female rats were divided randomly into four groups (YIGU capsule high dose group, medium dose group and low dose group; saline group), the drug-containing serum and control serum were prepared. (2) The new-born Sprague-Dawley rat osteoblasts were cultured with different YIGU capsule drug-containing serum at different concentrations and different exposure time. MTT method was used to observe proliferation of osteoblasts. (3) RT-PCR method was used to measure the relative IGF-I mRNA levels and ELISA method was used to measure IGF-I secretion at different exposure time. (4) ELISA method was used to measure IGF-I secretion at different exposure time. RESULTS: (1) Proliferation of osteoblasts was more than the control groups after 48, 72 and 96 h, respectively (P<0.01); (2) The relative IGF-I mRNA levels and IGF-I protein expression were higher than those in control group after 48, 72 and 96 h, respectively (P<0.01 or P<0.05). CONCLUSIONS: It was suggested that YIGU capsule drug-containing serum promoted proliferation, IGF-I mRNA and protein expression. These results may be parts of the mechanisms of YIGU capsule to prevent and treat osteoporosis.  相似文献   
117.
应用提取的鼠脂肪细胞膜分别免疫羊和鸡 ,所产生的抗血清用于 Wistar大鼠被动免疫。实验 1: 组腹腔注射羊正常血清 , 组腹腔注射羊抗鼠脂肪细胞膜抗血清 ,剂量均为 1m L /只 ,连续注射 4 d。结果表明 ,羊抗鼠脂肪细胞膜抗血清免疫促进了大鼠体增重 ,降低了体脂沉积 ,与对照组相比 ,7周末体重增加 6 .35 % (P<0 .0 5 ) ,饲料摄入增加6 .85 % (P<0 .0 1) ,料重比 (F/G)提高 4 5 .0 0 % (P<0 .0 5 ) ;肾周、附睾、网膜脂肪垫重量分别降低 2 3.92 % (P<0 .0 5 )、34.4 5 % (P<0 .0 5 )、0 .98% ,脂肪总量降低 2 0 .92 %。实验 2 :1组腹腔注射鸡正常血清 ,2组腹腔注射鸡抗鼠脂肪细胞膜抗血清 ,剂量均为 1m L/只 ,连续注射 4 d。结果表明 ,鸡抗鼠脂肪细胞膜抗血清免疫对大鼠的生长发育产生了不利影响 ,7周末 ,免疫大鼠平均体重较对照组减少 4 0 g(P<0 .0 5 ) ,饲料摄入显著降低 (P<0 .0 1) ;对体脂的沉积和血液中 TG和 FFA的影响没有规律 ,且无统计学意义  相似文献   
118.
运用免疫组织化学超敏 SP法对山羊胎儿脊髓发育中神经生长因子 (nerve growth factor,NGF)及其高亲和力受体 Tr KA的表达及其功能进行了系统的研究和探讨。结果显示 ,山羊胎儿脊髓灰质中存在 NGF及其受体 Tr KA,于 6周龄胚就可检测到 ,随胚龄增加 ,其表达范围及免疫反应着色程度逐渐增强。 NGF主要分布于腹角和背角的神经细胞 ,反应产物主要定位于胞质和突起 ;Tr KA的分布主要以腹角及胶状质为主 ,反应产物主要定位于胞核 ,后期胞质及突起也可见到阳性反应。在山羊胎儿脊髓白质中也可观察到 NGF及 Tr KA免疫阳性反应 ,其发育后期更为显著 ,阳性反应主要分布于神经胶质细胞核、神经纤维的轴索及雪旺氏细胞。结果提示 ,NGF不仅对交感和感觉神经元的发育起作用 ,而且还与腹角运动神经元的发育有关  相似文献   
119.
四川省外种猪雌激素受体基因对繁殖和生长性状的影响   总被引:1,自引:0,他引:1  
本试验以四川省外种母猪的 3个品种 (大约克、长白、杜洛克 )为研究对象 ,采用PCR RFLPs的方法检测其ESR基因的PvuⅡ多态性 ,分析了该基因与产仔数及生长性状之间的关系。结果表明 :初产胎次中 ,ESR基因型间总产仔数 (TNB)和产活仔数 (NBA)差异极显著 (P <0 .0 1) ,BB和AA纯合子间TNB和NBA分别相差 5 .97和 3.72头 ,基因加性效应分别为每个B基因 2 .98和 1.86头 ;对于经产胎次 ,总产仔数 (TNB)在AA、AB基因型与BB基因型的差异达到 0 .0 1的极显著水平 ,产活仔数 (NBA)在AA基因型与BB基因型间显著差异 (P <0 .0 5 ) ,TNB和NBA母猪每窝BB纯合子比AA纯合子分别多 3.6 8和 2 .89头 ,基因的加性效应为每个B基因分别为 1.84和 1.4 4头。头胎和经产胎次中ESR基因型在初生窝重、2 0日龄头数和窝重、30 / 4 5日龄头数和窝重以及 70日龄窝重之间的差异普遍不显著 (P >0 .0 5 ) ,但是以上 6个性状在ESR基因的 3种基因型间存在BB >AB >AA的趋势  相似文献   
120.
饲喂柠条对肉兔生产性能及胴体性状影响的研究   总被引:2,自引:0,他引:2  
选用45~48日龄平均体重1050g的伊普吕(父系)幼兔64只,分成4组,进行柠条饲喂肉兔试验.对照组饲粮中谷草比例为33.4%,试验Ⅰ、Ⅱ、Ⅲ组分别用10%、20%和33.4%柠条替代等比例谷草.48天试验结果为:对照组、试验Ⅰ、Ⅱ、Ⅲ组日均采食量、日增重、料肉比分别为89.84g、92.95g、90.77g、79.95g,22.60g、23.30g、19.24g、17.90g,3.98:1、3.99:1、4.72:1、4.47:1.试验Ⅰ、Ⅱ组与对照组日采食量、日增重、料肉比差异不显著(P>0.05);屠宰试验结果为:试验Ⅰ、Ⅱ与对照组在胴体重、屠宰率、净肉率、肉骨比、心脏、肺脏、肝脏、肾脏等指标方面无显著差异(P>0.05);而试验Ⅲ组日采食量、日增重均显著较对照组差(P<0.05).试验结果表明:生长肉兔饲粮中添加10%~20%柠条粉是可行的,且饲粮成本较低.  相似文献   
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