Apparent digestible protein,energy and amino acid availability of three plant proteins in Florida pompano,Trachinotus carolinus L. in seawater and low‐salinity water

Two experiments were conducted with Florida pompano, Trachinotus carolinus L. at 3 and 28 g L^?1 salinity to determine apparent crude protein digestibility (ACPD), energy digestibility (AED) and amino acid availability (AAAA) from soybean meal (SBM), soy protein isolate (SPI) and corn gluten meal (CGM). Mean AAAA was similar to ACPD. In fish adapted to 3 g L^?1 salinity, they were 81.2% and 81.9% (CGM), 93.6% and 92.2% (SBM), 93.8% and 93.1% (SPI) for AAAA and ACPD respectively. In fish adapted to 28 g L^?1, they were 84.5% and 83.4% (CGM), 86.5% and 87.1% (SBM), and 83.4% and 85.0% (SPI) for AAAA and ACPD respectively. The AED was highest for SPI and lowest for SBM and inversely related to carbohydrate. The ACPD, AED and AAAA of soy products appeared to be lower in high salinity, whereas CGM was unaffected. The data suggest that SBM, SPI and CGM should be further evaluated as partial fishmeal replacements in Florida pompano diets. Application of the generated coefficients can be used to develop well‐balanced, low‐cost diets for Florida pompano reared in low salinity or seawater.     

二种佐剂对哈氏弧菌OMPC-溶藻弧菌LPS偶联疫苗的增效作用

制备哈氏弧菌SpGY020601外膜蛋白复合物(OMPC)和溶藻弧菌EpGS021001脂多糖(LPS)偶联疫苗,设置偶联疫苗组、偶联疫苗 ISA763佐剂组、偶联疫苗 β-葡聚糖佐剂(DEAE)组以及生理盐水对照组,并以相同程序免疫卵型鲳鲹。溶菌酶活性检测、微量凝集反应试验结果显示:各疫苗组血清中的溶菌酶含量无显著差异(P>0.05),但均远高于生理盐水对照组(P<0.05);ISA763佐剂组抗哈氏弧菌SpGY020601抗体滴度较其他组出现的早,抗溶藻弧菌EpGS021001抗体滴度较其他组持续时间长;β-葡聚糖佐剂组次之,其次是无佐剂组,对照组始终未检测到抗体。各组分为腹腔注射攻毒与浸泡攻毒,对EpGS021001的攻击,ISA763佐剂组相对保护率分别为90%和70%,高于β-葡聚糖佐剂组的85%和55%,以及无佐剂组的80%和55%;对SpGY020601的攻击,ISA763佐剂组相对保护率分别为95%和80%,高于或相当于β-葡聚糖佐剂组的95%和75%,以及无佐剂组的90%和75%。     

Effect of dietary vitamin B6 supplementation on growth and intestinal microflora of juvenile golden pompano (Trachinotus ovatus)

In order to investigate the dietary vitamin B₆ (VB₆) requirement for juvenile golden pompano, an experiment of six different diets with six dietary VB₆ levels (0, 3.75, 7.47, 10.6, 13.7 and 18.5 mg/kg) was conducted. The results indicated that the content of dietary VB₆ significantly increased weight gain rate (WGR), specific growth rate (SGR) and feed conversion ratio (FCR; p < 0.05). Golden pompano fed diet containing 7.47 mg/kg VB₆ achieved the maximum ALT, AST, GPx, hepatic VB₆ concentrations as well as the minimum MDA, however, the ALT, AST, GPx and hepatic VB₆ concentrations decreased while MDA increased with a further increase in dieatry VB₆. The highest values of GR and POD occurred at the 10.6 and 13.7 mg/kg dietary VB₆ (p < 0.05) respectively. A diet supplemented with 7.47 mg/kg VB₆ increased intestinal Na⁺, K⁺‐ATPase, Chymotrypsin, γ‐glutamyl transpeptidase, creatine kinase and amylase activities (p < 0.05). The relative abundance of Tenericutes and Bacteroidetes decreased while Proteobacteria and Firmicutes improved with an increase in dietary VB₆ levels up to 7.47 mg/kg. Quadratic regression analysis on WGR, hepatic VB₆ concentrations, AST and ALT indicated that the optimum dietary VB₆ levels for juvenile golden pompano were 8.84–9.28 mg/kg.     

纳税申报电子信息采集系统设计

电子报税是我国税制改革的一个重要组成部分,根据电子报税的具体要求确定了系统的功能模块、开发及运行平台和网络拓扑结构,并按照系统工程原则,用现代软件技术设计了纳税申报电子信息采集系统。     

金针菇菌糠发酵床对生态养猪效果的影响

[目的]解决发酵床垫料来源问题,降低发酵床制作成本。[方法]以金针菇菌糠代替部分锯末制作发酵床,研究发酵床垫料配比对其温度和生猪育肥性能的影响,并分析金针菇菌糠在发酵床养殖过程中的应用前景。[结果]金针菇菌糠发酵床不会影响发酵床的发酵效果及生猪的育肥性能,有效降低了发酵床的制作成本。菌糠发酵床的最佳配方为:锯末20%、菌糠60%、稻壳20%。[结论]菌糠发酵床不仅有效解决了食用菌菌糠的环境污染问题,还可以极大降低发酵床的制作成本,可应用于该地区的发酵床养猪生产中。     

雌雄福寿螺摄食生长及形态性状差异研究

福寿螺是首批列入我国外来入侵物种“黑名单”的生物,已对我国南方水稻生产造成严重危害。通过室 内投喂实验测定了雌雄福寿螺摄食生长的差异,并通过“外采样测定了雌雄螺形态性状差异。结果显示院在分开饲 养足食投喂30 d后发现,雌螺的生长速度为雄螺的2.88 倍,这主要与雌螺有着更大的摄食量和更高的食物转化效 率有关。在共同饲养但限食投喂30 d后,雌螺的壳高增长率和体重增长率均大于雄螺,但未达到显著差异;雄螺的壳 高/壳宽、壳高/体重极显著大于雌螺（P<0.001）,壳口长/壳口宽极显著小于雌螺（P<0.001）。     

纳税申报电子信息采集系统设计

电子报税是我国税制改革的一个重要组成部分,根据电子报税的具体要求确定了系统的功能模块、开发及运行平台和网络拓扑结构,并按照系统工程原则,用现代软件技术设计了纳税申报电子信息采集系统。     

洪洞金丝小枣丰产管理技术调查

通过实践与调查，对洪洞金丝小枣的丰产管理技术作了系统总结。该丰产管理技术具有方法简便、适用性强、成本低等特点。只要适时把好各技术关，定能达到稳产、高产的目的。     

广西绿化植物黄素梅病虫害调查初报

为了解绿化植物黄素梅栽培中的病虫害危害情况,2006~2007年在广西的几个主要城市对黄素梅病虫害进行了系统调查,发现危害黄素梅的病虫害共有9种,分别是日本龟蜡蚧、棉蚜虫、温室白粉虱、黄蓟马、黄尾毒蛾、灰巴蜗牛、煤污病、中国菟丝子和1种叶斑病等。黄素梅病虫害总体发生数量少、危害程度轻,是一种优良的绿化树种,可进一步扩大种植。     

Effect of levels and sources of nitrogen on concentration and uptake of nitrogen by a high yielding variety and a hybrid of rice

Field experiments were made on a sandy clay loam soil at the Indian Agricultural Research Institute, New Delhi to study the effect of levels and sources of nitrogen on concentration and uptake of nitrogen by a high yielding variety Pusa 834 and a hybrid PRH3 of rice. Nitrogen concentration in hybrid PRH 3 remained lower than in Pusa 834, but N uptake was significantly more in the hybrid PRH 3. Nitrogen fertilization increased N concentration as well as N uptake by rice. At 30 days after transplanting (DAT) N uptake was more in Pusa 834, but at 60 DAT and at harvest hybrid PRH 3 recorded significantly more N uptake than Pusa 834. Use of neem oil blended urea (PNGU) and neem coated urea (NCU) increased N concentration and uptake by rice in both Pusa 834 and hybrid PRH 3. Use of neem coated/blended urea is recommended for rice.