Potential gene flow from transgenic rice (Oryza sativa L.) to different weedy rice (Oryza sativa f. spontanea) accessions based on reproductive compatibility

BACKGROUND: The possibility of gene flow from transgenic crops to wild relatives may be affected by reproductive capacity between them. The potential gene flow from two transgenic rice lines containing the bar gene to five accessions of weedy rice (WR1–WR5) was determined through examination of reproductive compatibility under controlled pollination. RESULTS: The pollen grain germination of two transgenic rice lines on the stigma of all weedy rice, rice pollen tube growth down the style and entry into the weedy rice ovary were similar to self‐pollination in weedy rice. However, delayed double fertilisation and embryo abortion in crosses between WR2 and Y0003 were observed. Seed sets between transgenic rice lines and weedy rice varied from 8 to 76%. Although repeated pollination increased seed set significantly, the rank of the seed set between the weedy rice accessions and rice lines was not changed. The germination rates of F₁ hybrids were similar or greater compared with respective females. All F₁ plants expressed glufosinate resistance in the presence of glufosinate selection pressure. CONCLUSIONS: The frequency of gene flow between different weedy rice accessions and transgenic herbicide‐resistant rice may differ owing to different reproductive compatibility. This result suggests that, when wild relatives are selected as experimental materials for assessing the gene flow of transgenic rice, it is necessary to address the compatibility between transgenic rice and wild relatives. Copyright © 2009 Society of Chemical Industry     

Prediction of irrigation return flows through a hierarchical modeling approach

The quantity of water available for irrigation is getting scarce in many countries and it assumes great importance for assured crop production, especially in view of the erratic behavior of the monsoon. Thus, there is a pressing need to improve the water efficiency of irrigation systems. One-way of improving the efficiency of the irrigation system is reusing the return flow from the irrigation system. This task requires quantification of return flow, which still remains as a grey area in irrigation water management. The estimation of return flow from the irrigation system is usually obtained using thumb rules depending upon the site-specific conditions like command area conditions and soil properties. In this paper, a hierarchical modeling technique, namely, regression tree is developed for return flow estimation. Regression tree is built through binary recursive partitioning. The effective rainfall, inflow, consumptive water demand, and percolation loss are taken as predictor variables and return flow is treated as the target variable. The applicability of the hierarchical model is demonstrated through a case study of Periyar-Vaigai Irrigation System in Tamil Nadu, India. The model performance shows a good match between the simulated and the field measured return flow values. Results of statistical analysis indicated that the correlation coefficients are high for both single as well as double crop seasons.     

Estimation of irrigation return flow from paddy fields considering the soil moisture

The objective of this study was to estimate irrigation return flow in irrigated paddy fields considering the soil moisture. The proposed model was applied to examine its feasibility with regard to the growing period of rice. Simulation results showed a good agreement between the observed and simulated values: root mean square error (RMSE) of 6.05-7.27 mm day⁻¹, coefficient of determination (R²) of 0.72-0.73, and coefficient of efficiency (E) of 0.54-0.55. The estimated average annual irrigation return flow during the period from 1998 to 2001 was 306.2 mm, which was approximately 25.7% of the annual irrigation amounts. Of this annual irrigation return flow, 14.1% was attributable to quick and 11.6% to delayed return flow. These results indicate that considerable amounts of irrigation water in the paddy fields were returned to streams and canals by surface runoff and groundwater discharge. The modeling assessment method proposed in this study can be used to manage agriculture water and estimate irrigation return flow under different hydrological and water management conditions.     

动边界同心环状缝隙流研究

为完善同心环状缝隙流理论,采用理论分析与模型试验相结合的方法,分析了圆柱体从静止到起动再到运行过程中同心环状缝隙流速的分布特点。得出圆柱体的速度、缝隙宽度以及流量对环状缝隙流的分布和大小的影响。环状缝隙流速随缝隙宽度的增大呈现先增大后减小的趋势,缝隙宽度约在2 cm附近时,缝隙流速最大;流量越大,环状缝隙流速就越大;圆柱体的速度越大,缝隙流速也越大;环状缝隙流速在与管内水流速度和圆柱体速度相交之前最大,相交之后最小。同时建立了动边界条件下的同心环状缝隙流数学模型,计算结果与试验基本一致,最大相对误差不超过8.5%,说明该数学模型可行。     

The Fluorescent Characterization and Analysis of Two Brucella Attenuated Vaccine Infecting Mouse Macrophagocyte

The experiment was conducted to discuss the difference of binding time of green fluorescent protein B.melitensis M5 (GFP-M5) and B.abortus S19 (GFP-S19) infecting the mouse macrophagocyte (RAW264.7),lysosome,endoplasmic reticulum and golgi body in the initial stage and compare the binding rate of GFP-M5,GFP-S19 with organelle in different timeline,respectively,by confocal laser scanning microscope (CLSM) and flow cytometry.The result showed that GFP-M5 and GFP-S19 were successfully constructed.The intracellular survival ability of Brucella M5,Brucella S19,GFP-M5 and GFP-S19 were not obvisouly affected after infecting RAW264.7.GFP-M5 and GFP-S19 could enter the macrophagocyte in 30 mins,and in 2 h the Brucella could reach lysosome,endoplasmic reticulum and golgi body.In addition,the binding time for two attenuated vaccine did not show differences in 1,2,3 and 4 h.The content of GFP⁺ cell produced by RAW264.7 infected by GFP-M5 and GFP-S19 did not show significant differences (P>0.05).Therefore,the two strains did not have significant differences in the invasion ability in the initial stage of infecting host cell.     

Simulation on the Characteristics of Flow Pattern in Sprial uP-flow Reactor

In order to analyze the reason why the Spiral Up-flow Reactor system has excellent removal effect of COD, N and P, the authors use PHOENICS, a computational fluid dynamics program, to investigate the hydraulic characteristics and flow model of the anaerobic part of Spiral uP-flow reactor. The result shows that the fluid in the reactor unit flowed upward by the spiral way, and that flow pattern was combined by plug flow and complete-mix flow, and that the percentage of plug flow was about sixty-five percent. Spiral Up-flow Reactor units the Characteristics of PFR and CSTR, which makes it work well.     

NETWORK FEOW APPROACH TO RELIABILITY EVALUATION IN IARGE SCALE NETWORKS BY MIXING INCREASING ELOW WITH DECREASING FLOW

Based on the maximum flow principle about networks, a network flow approach to reliability evaluation in large scale netwerks ty mixing inereasing flow with decreasing flow is proposed in this paper.This approach gets the initial flow of each branch in a r.etwork associated with a state of the retwork by decreasing flows of some branches correlative to another state of the network.As a result, the number of flow compute required can be reduced enormously.The numerical example shows that the approach is powerful. The comparison of this approach with the minimal cutset one is also given in this paper.     

Artificial Neural Networks Models for Flow Stress during High Temperature Plastic Deformation of Al-Li Alloy

Using gained experimental data to develop the models of stable flow stresses at high temperature plastic deformation by statistical methods for alloy materials, precision of the models is poor and at the same time the processes of modeling are complicated with great workload. On the basis of the data obtained on Gleeble-1500 Thermal Simulator,the predicting models for the relation between stable flow stress during high temperature plastic deformation and deformation strain, strain rate and temperature for 1420 Al-Li alloy have been developed with BP Artificial Neural Network method. The results show that the model on basis of BPNN is practical and it reflects the real feature of the deforming process. It states that the difference between the real value and the output of the model is in order of 5 percent.     

Numerical Simulation of Flow field and Temperature field in Combustion Chamber Widened Suddenly

The flowfield and temperature field are numerically Simulated by the mathematical model of the fluid flow and heat transfer in circular combustion chamber widened suddenly. The distributions and influence factors of the flow field and temperature field are studied in it. The results have the guiding significance for the design of combustion chamber. The mathematical models can serve as the quantitative analysis methods for design.