16S rRNA在兽医病原菌分类鉴定中的应用

介绍16S rRNA基因的特点，阐述其用于病原菌分类鉴定的基本原理，综述其在兽医病原菌分类鉴定中的应用。     

鹅出血性坏死性肝炎的初步研究

本病为雏鹅的一种病毒性传染病，病毒为球形，无囊膜，直径为76－86nm大小的颗粒，属于呼肠孤病毒科鹅呼肠孤病毒。能在发育良好的鹅胚等禽胚内复制，致死胚胎，并具有特征性病变。患病雏鹅以出血性坏死性肝炎为特征性的大体病理变化和显微病理变化。     

花生区组间杂种二代性状鉴定

花生区组四粒红与根茎区组Arachis glabrata杂交果针经离体培养克服了不亲和性,获得杂种。杂种二代性状出现疯狂分离,但有些性状仍分别倾向双亲。经叶片过氧化物同工酶分析,酶带的条数、宽窄、活性强弱及迁移率,杂种与双亲有明显差异,也有共同之处,初步证明了杂种的真实性。     

磷化铝间歇熏蒸防治鱼粉螨类的研究

本研究介绍了饲料鱼粉螨类的有效防治方法．采用11．5g／m2的磷化铝片剂，分两次间歇熏蒸大量孳生螨类和昆虫的棉纱布袋和编织袋包装的鱼粉，达到了有效杀灭鱼粉中大量孳生的粉尘螨、跗蠊螨、齿蠊螨、河野脂螨、食根嗜木螨、马六甲肉食螨、转开肉食螨、特氏肉食螨等8种螨类和烟草甲、白腹皮蠹、拟白腹皮蠹、赤颈郭公虫、赤足郭公虫、黑菌虫的效果。     

Nuclear transfer in loach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion

To study nuclear transfer in the loach (Paramisgurnus dabryanus Sauvage), blastula and gastrula cells were fused with UV-inactivated oocytes by cell-to-cell electrofusion. To facilitate nuclear transfer, blastula and gastrula cells were cultured or incubated at 4 °C in different solutions. TC-199 medium supplemented with 20% calf serum was the best culture solution, and effectively retained the totipotence of blastula or gastrula cells for up to 10 days. It was found that gastrula cells incubated at 4 °C had the same totipotence as blastula cells. The optimal UV dosage for inactivation of the oocyte chromatin was 180–240 mJ cm⁻². Electrofusion was carried out in a cone-shaped fusion chamber, which permitted the recipient oocyte and the donor blastula cell to contact one another. The electrofusion procedure resulted in a 10% success rate of normal-appearing fish. Genetic analysis indicated that the nuclear material originated from the donor cell (blastomere) and the oocyte pronucleus did not take part in development.     

Application of acoustic Doppler current profiler combined with a scientific echo sounder for krill Euphausia pacifica density estimation

ABSTRACT:   An application of the acoustic Doppler current profiler (ADCP, 153.6 kHz) in combination with a scientific echo sounder (EK60, 38 and 200 kHz) was investigated to estimate the density of krill Euphausia pacifica . The acoustic backscattering strength from sound scattering layers was compared with biomass estimates from midwater trawls. Euphausia pacifica was targeted among mixed species populations in the sound scattering layer in the offshore Funka Bay area of Hokkaido, Japan. The frequency characteristics of acoustic backscattering by krill were calculated using a distorted wave Born approximation scattering model at three frequencies. Krill aggregations identified from the EK60 data were extracted as the mean volume backscattering strength difference between two frequencies. They were then used to identify similar aggregations in the ADCP data by matching observation times and depths for the two methods, which were applied simultaneously. Results from the comparison of the mean volume backscattering strength and the density calculated from the ADCP and EK60 showed that ADCP can be used to measure density and spatial–temporal distribution of krill aggregations. Current speed and direction at the study site were found to be 16.1 cm/s and 187.0°, respectively, and krill speed and direction (including the current component) were found to be 19.8 cm/s and 172.2°, respectively. Based on the ADCP data, the net speed and direction of the krill aggregations were found to be 5.9 cm/s and 128.0°, respectively.     

农业科研立题和成果鉴定的情报查新

阐述了农业科研立题,成果鉴定的情报查新的必然性及重要意义,并对查新工作提出了几点建议。     

基于系统辩识的PID参数自整定

This paper presented a metnod of self-determining PID parameters by using system idenification and gave a engineering sample.     

表达HAV结构基因的重组腺病毒鉴定及细胞病变分析

利用RT-PCR方法,从HAV-L8株的RNA中扩增出结构基因(vp3 vp1),克隆到穿梭质粒pXCX2NotI上。采用磷酸钙-DNA共沉淀技术,将腺病毒载体pJM17与pXCX2-CMV-HAV共转染293细胞。在光学显微镜下观察到明显的细胞病变(CPE);经RT-PCR、免疫荧光染色和蛋白印迹鉴定证明HAV的结构基因已插入到腺病毒基因组中;在电子显微镜下观察发现有二十面体的病毒颗粒。以上结果表明获得了重组腺病毒rAdHAV,纯化后的rAdHAV滴度为1×109.0TCID50/mL。     

香菇简易开放式栽培高产菌株的早期鉴定

试验对５个香菇菌株进行简易开放式栽培并观察其特性，发现菌丝生长速度、锁状联合数与生物学效率相关性不密切，香菇的酶活性，子实体形成所需时间、子实体单重、菌盖重与生物学效率密切相关。