不同进食水平对生茸期梅花鹿能量代谢的影响

本试验采用梯度饲养试验、消化代谢试验及呼吸测热试验等方法 ,对成年梅花鹿生茸期能量代谢规律进行了研究。结果指出 :①随着能量进食水平的提高 ,梅花鹿体沉积能显著增加 ;②梅花鹿日产热量与进食能量水平呈强直线相关 ;③甲烷能产生量随着能量进食水平的增加而增加。     

北京黑猪和长白猪脂肪代谢的比较

猪在断奶后的生长期内,体内脂肪逐渐增加,和其它家畜相比,猪更倾向沉积体脂。但猪体脂的沉积还要受遗传、年龄、日粮营养水平等因素的影响。在饲粮消化能量(DE)水平保持在3000—3200Kcal/kg,蛋白质含量为14％—15％的饲养条件下,我们分别测定了纯系北京黑猪(肉脂兼用型,平均瘦肉率为51％)和纯系长白猪(瘦肉型猪、平均瘦肉率为62％)的某些脂肪合成酶活性和脂肪代谢的一些生化指标。北京黑猪(公母各半)脂肪组织(背膘)中苹果酸酶(ME),葡萄糖—6—磷酸脱氢酶(G—6—PDH),异柠檬酸脱氢酶(ICDH)三种NADPH—生成酶活性随体重增加而升高,差异极其显著(P<0.001)。体重90kg的酶活性比56kg约高三倍。脂纺组织中上述NADPH生成酶活性北京黑猪比长白猪(体重均为90kg,公母各半)高一倍(P<0.001)。给北京黑猪(30头,公母各半,起始体重为60kg)注谢重组猪生长激素(rPGH,每天每头2mg,连续注谢28天),除表现生长加快,脂肪率下降约21％—23％外,其脂肪组织中NADPH生成酶活性降低十分明显(P<0.001)。苹果酸酶,葡萄糖—6—磷酶脱氢酶,异柠檬酸脱氢酶,三种酶总活性比对照组降低20％。血精中三酰甘油,游离脂肪酸,总胆固醇,高密度脂蛋白(HDL)胆固醇、乳糜微粒,极低密度脂蛋白(VLDL)胆固醇含量,6月龄时,北京黑猪明显高于长白猪(P<0.001—0.0005);而4月龄时,北京黑猪血清中三酰甘油,高密度脂蛋白胆固酶含量则均明显低于长白猪。北京黑猪血清中三酰甘油,高密度脂蛋白胆固醇含量,随月龄(4月龄—6月龄)增长而增加(P<0.0005)。而长白猪则相反,随月龄而呈下降趋势(P<0.05—0.0005)。两种猪的脂蛋白电泳和含量测定未见有年龄和品种间的差异。     

薄层扫描法测定人工诱生牛黄中主要成分的含量

应用岛津CS-910型薄层扫描仪测定了5份人工诱生牛黄和3份天然牛黄中胆酸(CA)、去氧胆酸(DCA)和胆红素(BR)的含量。CA和DCA用荧光扫描,λ_(ex)470nm和λ_(em)550nm;BR用可见光扫描,λ_S402nm和λ_R650nm。测定结果表明,前者中CA和DCA的含量高于后者;BR的含量则前者低于后者。     

The Effect of Chilling Maize Shoots and Roots on the Electric Potential, Composition of Fatty Acids and the ATPase Activity in the Non-chilled Organs of Seedlings

The effect of separate chilling (5°C for 2 and 4 days) on the shoots and roots of two maize hybrid seedlings on the electric potential, the composition of fatty acids and the ATPase activity in the microsomes of non-chilled leaves and roots (20°C) was investigated. It has been found that the low temperatures induced on the non-chilled organs gave similar changes to organs exposed to a reduced temperature. The changes consisted of a parallel depolarization of leaf and root membranes and similarly a decrease in the ATPase activity as well as changes in the unsaturation of the membranes' fatty acids. The reduction of the acid content 18:2 was particularly significant, which in the leaf microsomes correlated with a drop in the ATPase activity.
The similarity between the reactions of the chilled and non-chilled parts of the plant gives evidence of a communication' between the organs which may facilitate a coordinated reaction of the entire organism to stress. The background for these phenomena could be die action potential of die chilled parts of seedlings which, in the non-chilled parts, might produce changes in the structure of biomembranes and in the activity of enzymes.     

家蚕丝素蛋白对大鼠胆固醇代射的影响

研究了经酸解后制得的家蚕丝素蛋白氨基酸组成以及对高血脂实验鼠血清和肝脏内胆固醇及转氨酶的活性变化的影响。结果表明：所得的家蚕丝素蛋白粉氨基酸组成丰富，其中甘氨酸、丙氨酸、丝氨酸和酪氨酸含量占全部氨基酸总量的95%；家蚕丝素蛋白能显著地降低血清胆固醇含量和谷草转氨酶及谷丙转氨酶活性，提高动物肝组织中的胆固醇浓度和谷丙转氨酶的活性。     

家禽核酸疫苗的研究进展

核酸疫苗是当今疫苗研究的最前沿领域，本文就核酸疫苗在家禽传染病中的应用做一综述。     

广州香蕉束顶病毒分离和核酸鉴定

经液氮冷冻、差速离心和蔗糖梯度离心，从广州地区香蕉束顶病株中获得大小为25nm的病毒颗粒，该病毒与香蕉束顶病毒单克隆兔抗血清呈阳性反应，该病毒核酸可被Dnase和S1核酸酶降解，但不能被RNase降解，属于ssDNA ；人子量大小约为1165碱基。     

Photosynthate Unloading from Seed Coats of Phaseolus vulgaris L. an Attempt to Define Conditions Required for Stimulation by Abscisic Acid and Benzylamino Purine

The instablity of the stimulation of photosynthate unloading from seed coats of Phaseolus vulgaris by abscisic acid (ABA ) and benzylamino purine ( BAP ) was studied in terms of experimental conditions. ABA stimulated photosynthate unloading at pH 6 and pH 8 without close dependence upon pH. ABA at a concentration range of 10-5-10-4 mol/L displayed stimulatory effect. However, BAP revealed no effect at a concentration range of 10-6-10-4 mol/L. Experiments designed with different transport time of 14C-photosynthate indicated that ABA might act at the plasma membrane of the unloading cells. Reducing endogenous ABA level by diminishing leaf area did not facilitate the manifestation of exogenous ABA function. Potassium ion stimulated photosynthate unloading from seed coats with highest promotion at 100 mmol/L K . However, no dependence of ABA stimulation of photosynthate unloading on K was found.     

Essential Fatty Acids and the Cat

Abstract— The physiology of essential fatty acid metabolism in the cat is reviewed. Emphasis is placed on those aspects of the n:6 and n:3 fatty acids, their metabolites and interactions, which relate primarily to the skin. The functional roles, if known, of the fatty acids are discussed. Recent clinical research into the use of essential fatty acid supplements in the management of feline dermatoses is presented. Current indications for the therapeutic supplementation with essential fatty acids are summarised.