毛竹cab-PhE11基因的克隆与序列分析

采用RT-PCR技术和RACE-PCR技术,从毛竹(Phyllostachys edulis)中分离了捕光叶绿素a/b结合蛋白基因cDNA编码区全长,命名为cab-PhE11(GenBank登记号:EU327783)。该基因全长1154 bp,编码区cDNA全长753 bp,编码250个氨基酸。生物信息学分析表明,在cab-PhE11编码的蛋白第62~239位包括典型的捕光叶绿素a/b结合蛋白功能域,还包含1个N-糖基化位点、1个酪氨酸激酶Ⅱ磷酸化位点、5个N-肉豆蔻酸化位点以及1个丙氨酸富集区。序列相似性分析结果表明,cab-PhE11编码的氨基酸序列与玉米、绿豆、拟南芥、烟草等的cab基因有较高的相似性,都在80%以上,该基因属于lhcb6类基因。     

粉碎粒度对粗蛋白测定结果的影响

将同一饲料样品按4分法缩分为3份,1份按GB／T6432-94制备样品,另2份过20目和40目筛片粉碎制备样品,按姜懋武提出的改进方法测定粗蛋白（CP）,研究样品的粉碎粒度对CP测定结果的影响。结果表明,不同粉碎粒度制备样品测定的CP结果,不同饲料样品间没有表现出相同的规律。在测定的11个样品中,只有3个样品（仔猪前期颗粒料、生长育肥猪浓缩料和肉用仔鸡后期颗粒料）的CP测定值不受粉碎粒度变化的影响（P〉0．05）,其他8个样品的CP测定值均受饲料粉碎粒度的影响（P〈0．05或P〈0．01）,但不同品种间表现的规律不尽相同。玉米、小麦和大豆等籽实类饲料原料,除玉米20目和40目间无显著差异外,其余不同粉碎粒度间均表现出差异极显著。饼粕类饲料原料中,豆粕与棉粕60目组极显著高于20目和40目组,20目和40目组间差异不显著;花生粕与菜粕20目组显著高于40目和60目组。肉用仔鸡后期浓缩料40目和60目极显著高于20目,40目和60目间差异不显著。     

Aux/IAA基因家族鉴定及对大豆茎尖发育的调控作用分析

Aux/IAA基因家族在植物茎尖发育过程发挥重要作用.为了探究大豆Aux/IAA基因家族在大豆茎尖发育过程的调控作用,本文以拟南芥Aux/IAA基因家族蛋白序列为参照鉴定了大豆全基因组Aux/IAA家族基因,包括63个成员;然后以拟南芥、鹰嘴豆和大豆的Aux/IAA家族为研究对象,比对这些基因全长氨基酸序列并构建进化树...     

卵黄和低密度脂蛋白对冷冻保存绵羊精子胆固醇、磷脂含量的影响

为了探索绵羊冷冻精液受精率低的原因,在稀释液中用低密度脂蛋白代替卵黄冷冻绵羊精液,研究LDL对冷冻中精子脂类含量的影响。结果表明,以卵黄稀释液冷冻精液,精子胆固醇含量从鲜精的234．54nmol／10^9降低到163．76nmol／10^9精子,胆固醇与磷脂的比值（c／p）从0．54下降到0．36;以9g／100mL LDL代替卵黄,冷冻后胆固醇含量为243．73nmol／10^9精子,c／p值保持在0．50。研究得出在卵黄稀释液中冷冻绵羊精液,精子胆固醇流失,c／p值下降;以LDL代替卵黄,可有效保护精子胆固醇,避免流失,维持了精子c／p值。     

非1B/1R类型小麦雄性不育系KTSP3314A败育的生化机制

为了揭示K型非1B/1R类型小麦不育系雄性败育的生化机制。利用K型非1B/1R类型小麦雄性不育系KTSP3314A,其同型保持系TSP3314B,以K型1B/1R类型小麦雄性不育系K3314A、其同型保持系3314B为对照,分别对其叶片和穗子在不同发育时期的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性和丙二醛(MDA)含量的变化进行测定。结果表明,K型非1B/1R类型小麦不育系中SOD,POD及CAT活性变化与K型1B/1R类型小麦雄性不育系酶活性变化一致,K型非1B/1R类型雄性不育小麦叶片和穗子的超氧化物歧化酶(SOD)后期则较低,过氧化氢酶(CAT)、过氧化物酶(POD)的活性后期较高,丙二醛(MDA)含量后期较高。说明二者的雄性败育生化机制基本一致,但前者比后者败育的时期较晚。且超氧化物歧化酶(SOD)活性与雄性育性有关。     

不同类型有机物料的有机磷组成及生物有效性

[目的]研究不同有机物料的有机磷组成及其作为磷源施用后的供磷能力,为化肥磷的有机替代奠定理论基础.[方法]供试有机物料包括粪肥类(猪粪、羊粪)、绿肥类(豌豆、苜蓿和绿豆)、秸秆类(小麦秸秆、玉米秸秆和油菜秸秆).分析了8种有机物料的全磷、有机磷含量和C/P值,采用Bowman-Cole方法测定了有机磷中的活性(LOP)...     

Protective effect of C1q/tumor necrosis factor-related protein 3 on vascular endothelium in rats with hyperuricemia

AIM To study whether C1q/tumor necrosis factor （TNF）-related protein 3 （CTRP3）protect vascular endothelium in rats with hyperuricemia and its potential mechanisms. METHODS An animal model of hyperuricemia was established by using male SD rats drinking 10% fructose water （n=10）. The rats drinking normal water served as normal controls （n=10）. After 12 weeks, the rats were given a single injection with Ad-CTRP3 or Ad-GFP. The experiment was ended at 14th day after transfection.The serum levels of uric acid and nitric oxide （NO） were evaluated. The serum contents of TNF-α and interleukin-6 （IL-6） were measured by ELISA. HE staining and TUNEL assay were used to assess the morphological changes of intima and apoptosis of endothelial cells in thoracic aorta, respectively. The mRNA levels of endothelial nitric oxide synthase （eNOS）, TNF-α and IL-6 were detected by RT-qPCR. The protein levels of CTRP3 and Toll-like receptor 4 （TLR4） were determined by Western blot. RESULTS Compared with normal control group, the rats with hyperuricemia showed lower CTRP3 and higher TLR4 protein levels in the thoracic aorta （P<0.05）. Hyperuricemic rats had higher serum contents of uric acid, TNF-α and IL-6 （P<0.05）. Also, the intima structure disturbance of thoracic aorta, increased apoptotic rate, higher mRNA levels of TNF-α and IL-6 as well as lower mRNA levels of eNOS were observed （P<0.05）. By contrast, CTRP3 over-expression decreased TLR4 protein levels, reduced inflammatory cytokines, and obviously improved the morphology and function of thoracic aorta in the rats with hyperuricemia. CONCLUSION CTRP3 protect vascular endothelium in rats with hyperuricemia maybe via down-regulation of TLR4- mediated inflammatory signaling pathway.     

Dihydroartemisinin enhances radiotherapy efficiency in H22 cell tumor-bearing mice by regulating PI3K/AKT signaling pathway

AIM To study the effect of dihydroartemisinin （DHA） on the radiotherapy efficiency in hepatocellular carcinoma H22 cell tumor-bearing mice and the role of phosphatidylinositol 3-kinase （PI3K）/protein kinase B （AKT） signaling pathway in this process. METHODS A model of H22 cell tumor-bearing mice was established. The mice was divided into model group, single radiotherapy group, 5-fluorouracil （5-FU） group, and low-, medium- and high-dose DHA groups. The body weight and tumor volume in each group were measured every other day. At the end of administration, blood was collected from the tail of the mice and the animals were killed by neck removal immediately. The synergistic effect of DHA on radiotherapy was determined, and tumor growth inhibitory rate was calculated. The degree of lymphocyte transformation and natural killer （NK） cell activity were measured by MTT, the serum levels of interleukin-2 （IL-2） and IL-4 were measured by ELISA, and the protein levels of PI3K, AKT and p-AKT were determined by Western blot. RESULTS The H22 cell tumor-bearing mouse model was successfully constructed. Compared with model group, the TGT₃ （tumor growth time to reach 3 times of volume） of single radiotherapy group was remarkably increased （P<0.05）, while tumor weight, lymphocyte transformation degree, NK cell activity, IL-2 and IL-4 levels, PI3K protein level and AKT phosphorylation level were remarkably decreased （P<0.05）. Compared with single radiotherapy group, TGT₃, EF （enhancement factor）, tumor inhibitory rate, lymphocyte transformation degree, NK cell activity, IL-2 level and IL-4 level were increased with the increase in DHA dose （P<0.05）, and the PI3K protein level and AKT phosphorylation level were decreased （P<0.05）. CONCLUSION DHA may enhance the immunity of tumor-bearing mice by inhibiting the activity of PI3K/AKT signaling pathway, thereby enhancing the efficacy of radiotherapy.     

Curcumin attenuates rat lung ischemia/reperfusion injury by interfering with autophagy

AIM To investigate the role of curcumin （CUR） in lung ischemia/reperfusion （I/R） injury （LIRI） and its relationship with autophagy. METHODS 40 SD rats were randomly divided into sham operation （sham） group, I/R group, solvent （DMSO） group, CUR group and CUR+rapamycin （CUR-Rap） group. The rats were intraperitoneally injected with normal saline, DMSO, CUR or CUR+Rap before operation. After the rat LIRI model was established, the lung tissues were taken to measure W/D, TLW, IAR, and the contents of SOD and MDA were also measured to indicate the oxidative stress level. Light and electron microscopes were used to observed the morphology and ultrastrucure of lung tissues. The expression levels of autophagy-related proteins were determined by Western blot to evaluate autophagy levels. RESULTS Compared with sham group, wet weight/dry weight （W/D）, total lung water （TLW）, injured alveoli rate（IAR） and malondialdehyde （MDA） content in all other groups were increased, superoxide dismutase （SOD） activity was decreased, the levels of autophagy were increased （P<0.05）, and lung tissue injury and cell ultrastructural damage were aggravated in CUR group. Compared with DMSO group, W/D, TLW and IAR and MDA content were decreased, SOD activity was decreased, autophagy levels were also decreased （P<0.05）, and lung tissue and cell ultrastructural damage were attenuated. Compared with CUR group, W/D, TLW, IAR and MDA content were increased, SOD activity declined, the autophagy levels were increased （P<0.05）, and damage of lung tissues and cells were more serious in CUR-Rap group. CONCLUSION Curcumin attenuates the lung I/R injury in rats, and its mechanism may be related to the reduction of oxidative stress and the inhibition of autophagy.