Early development of the shortfin silverside Chirostoma humboldtianum (Valenciennes, 1835) (Atheriniformes: Atherinopsidae)

The shortfin silverside Chirostoma humboldtianum has been considered for culture in Mexico, but success has been limited by a poor knowledge of its early development. First synthesis of the early development of the shortfin silverside is presented to determine conditions suitable for rearing. Brooder maturation was induced through photothermal cycles. C. humboldtianum ova were fertilized in vitro. The eggs were incubated in reconstituted water (160-180 mg/L CaCO₃) at 18 °C and 5 gm of NaCl per litre. During the hatching day, 300 shortfin silversides were stocked and followed up until metamorphosis in order to establish the timing of exogenous feeding, changes in food type, growth and development during critical periods for survival, according to the theory of saltatory ontogeny. Free embryos hatched 12 days after fertilization at 18 °C. First critical point for survival is the beginning of exogenous feeding. Free embryos started mixed feeding on day four of post-hatching (dph), point of no-return was presented towards the end of mixed feeding on 6 dph, larval period began at six (dph) when the anus is opened, and metamorphosis to juvenile was presented at 65 dph with a SL of 19.34 ± 2.28 mm, when scales and fins were well developed. Differences in growth between periods were detected: free embryos growth slower than larvae but mouth size depicted a larger growth rate in the former. Cephalic length and mouth size were negatively related to standard length in embryos and larvae. Mouth size was positively related to cephalic length in free embryos but negative in larvae. Results suggest that during the free embryo phase, growth priorities are directed to the development of apparatuses and systems; whereas, during the larval period, energy is directed to growth in length, mouth size and development of fins, which allows them to increase their swimming velocity, grants them a greater capacity to obtain exogenous food and, in consequence, increases fitness for survival.     

大黄鱼一年两次性成熟发育特征的研究

选择春季产过卵的2^ 龄大黄鱼Pseudosciaena crocea(Richardson)在网箱中进行隔离培育，秋季从中挑选当年再次性成熟的亲鱼进行人工催产，比较春、秋两季的人工繁殖与仔、稚鱼培育效果，并定期观察卵巢及卵细胞的发育情况。实验表明，春季产过卵的同一批大黄鱼雌鱼，有72．1％的个体当年秋季可再次成熟，有58．8％的个体可以再次产卵，证实养殖大黄鱼具有一年“两熟”的性腺发育特征。     

Larval development in European hake (Merluccius merluccius L.) reared in a semi-intensive culture system

Eggs of European hake (Merluccius merluccius L.) were stripped from fish caught at sea. Larvae were kept under semi‐intensive conditions at around 12°C. In addition, eggs were incubated in single wells at 9.2, 12.7 and 14.5°C, where hatching, development and survival were closely examined. During the larval stage, a total of 299 larvae were sampled to follow development and growth. In addition a small number of juveniles were sampled. Larvae hatched approximately 4 days after fertilization, and were 2.9 mm in total length (TL). At 6‐day post hatching (dph), the larvae were 4.1 mm (TL), the jaw apparatus was developed, and the larvae had started to feed. Most of the growth during the early larval period is restricted to the head, and there is almost no increase in length for the first 3–4 weeks post hatching. Teeth and pelvic fins appear at 25 dph. Development of unpaired fins at approximately 30 dph marks the start of the larval–juvenile transition. Weaning to formulated feed was accomplished 50 dph, when external morphology was similar to that of adult hake.     

Influences of in-stream habitat and upstream land-use on site occupancy of the Kanawha darter (Etheostoma kanawhae): A narrowly distributed species from the New River (Upper Kanawha Basin)

1. Freshwater communities are threatened by the conversion of natural landscapes for urban and agricultural purposes. Changes to land use may disrupt stream nutrient and geomorphological processes and reduce water quality, increase sedimentation, and decrease habitat heterogeneity eventually leading to species loss and decreases in ecosystem productivity. Endemic species are frequently at greater risk of habitat-mediated fragmentation and extirpation due to their constrained distributions.
2. The Kanawha darter (Etheostoma kanawhae) is an understudied fish endemic to the New River Drainage in North Carolina and Virginia, USA. To investigate the potential effect(s) of land-use change on Kanawha darters, naïve occupancy was modelled using instream habitat characteristics and upstream forest cover.
3. Generalized linear models revealed that instream habitat and forest cover are reliable predictors of Kanawha darter site occupancy. Specifically, models demonstrated that occupancy increased in reaches with reduced stream width, velocity, and bedrock substrate but higher concentrations of coarse woody material. Kanawha darter occupancy was also positively associated with the extent of forest cover in upstream catchments.
4. Although Kanawha darters are not currently considered imperilled, most populations occurred in isolated reaches separated by large sections of unoccupied habitat. Continuing ex-urban development in riparian zones is likely to be the primary threat to Kanawha darters and other endemic species in this catchment. Resource managers and stakeholders should preserve forest cover in headwaters and occupied tributaries and protect or restore riparian zones along the main-stem South and North Forks of the New River to preserve high-quality habitat and enhance connectivity among isolated Kanawha darter populations.
5. As human populations in montane regions continue to grow, there is a need to understand how land-use change affects endemic freshwater species. This study further supports the importance of retaining forest cover as an effective strategy for protecting and restoring populations of endemic fishes in high-gradient streams.

     

Effects of weaning age and diets on ontogeny of digestive activities and structures of pikeperch (<Emphasis Type="Italic">Sander lucioperca</Emphasis>) larvae

Growth and ontogeny of digestive function were studied in pikeperch (Sander lucioperca) larvae weaned on artificial food at different ages. Three weaning treatments initiated respectively on day 9 (W9), day 15 (W15) or day 21 (W21) post-hatching (p.h.) were compared with a control group, fed Artemia nauplii from first feeding until the end of the rearing trial on day 36 p.h. The digestive enzyme activities and the ontogeny of digestive structures were investigated using enzymatic assays and histological methods. Growth of pikeperch larvae was significantly affected by precocious weaning. Pancreatic (trypsin and amylase) and intestinal (leucine-alanine peptidase, leucine aminopeptidase N and alkaline phosphatase) enzyme activities were detected from hatching onwards, increased at the moment of first feeding and then decreased. Pepsin secretion occurred at day 29 p. h. only, concurrently with the stomach development and differentiation of gastric glands. In the early weaning group (W9) the maturation process of intestinal enterocytes seems to be impaired and/or delayed and several signs of malnutrition were recorded. Except for alkaline phosphatase activity, no differences in enzyme activities and development of digestive structures were observed among the control, W21, and W15 groups. Moreover, at the end of the experiment, no differences in proteolytic activities were observed among larvae from the different treatments, indicating that, in surviving individuals, the digestive structures were properly developed and the larvae had acquired an adult mode of digestion. Based on the artificial diet used, our results suggested that pikeperch larvae can be weaned from day 15 p.h. without significant adverse effect on digestive capacities (except for alkaline phosphatase) or development of digestive tract, while earlier weaning impaired the onset of the maturation processes of the digestive system, both in terms of morphological structures and enzymatic activities.     

不同水分处理下液膜覆盖对夏玉米生长及产量的影响

针对塑料地膜对农业生产造成的严重污染问题,运用对照处理方法,探讨了液体地膜覆盖对夏玉米生长发育及产量等方面的影响。结果表明,液膜覆盖使夏玉米株高和茎粗均比对照偏大,其中,低水分处理下差异最为显著。夏玉米叶面积指数低水分处理时液膜覆盖在生育前期较对照偏大,生育后期偏小;而中、高水分下液膜覆盖叶面积指数在整个生育期内均比对照偏大。液膜覆盖使夏玉米光合速率和蒸腾速率显著增大,有利于光合产物的形成。低水分处理下液膜覆盖增产效果最好,增产率达到56.97%,产投比为1.695,高水分下液膜覆盖增产效果最差,增产率仅为15.57%,液膜覆盖产投比小于对照。研究认为,夏玉米液膜覆盖能有效抵抗干旱逆境从而达到节水增产的显著效果。     

基于光谱学和ARM的番茄叶绿素检测仪的开发

为了更加方便、简捷、快速的对温室番茄叶绿素含量进行检测,利用光谱分析技术,开发了一种用于预测温室番茄叶片叶绿素含量的新型作物长势仪。仪器选择527 nm和762 nm两个波长作为特征波长。由一个“Y”型光纤,两个硅光电池,一个信号处理单元,中心处理器及友好的人机交互界面组成。由番茄叶片反射回来的反射光经过“Y”型光纤到达硅光电池的表面,硅光电池可以将此光信号转换成电信号。然后经过信号处理单元的放大滤波转换成可以经CPU处理的电压信号。根据测量得到的光谱反射率可以计算出番茄叶片的光谱系数,再根据光谱系数预测     

深化对查新工作的认识 创新查新服务模式

结合查新工作经验,分析了当前兵团科技查新现状及主要需求,并就查新工作中存在的问题及原因进行了探讨,提出了对提高查新工作效率、保障查新工作质量、创新查新服务模式和开展查新增值服务的几点建议。     

基于参数传递的高校二级网站开发与研究

针对目前高校二级网站开发中应注意的问题,给合一个高校二级网站开发的实际项目,从技术选择、功能设计、功能实施等几个方面加以讨论,旨在提供一个功能开放、代码高效、管理方便的基于参数传递式的高校二级网站开发模式。     

腐植酸对水稻产量、品质及土壤特性的影响

在农业生产中,腐植酸具有促进生长、提高肥效、增强抗逆性、改善品质及改良土壤的作用.本文综述了腐植酸对水稻生长发育、产量形成、稻米品质以及土壤特性的影响,展望了腐植酸今后的应用研究,如与化肥适宜的配施比例、施用浓度等,以期为水稻优质、高产、稳产及高效栽培提供依据.