全文获取类型
收费全文 | 3996篇 |
免费 | 192篇 |
国内免费 | 441篇 |
专业分类
林业 | 67篇 |
农学 | 102篇 |
基础科学 | 6篇 |
145篇 | |
综合类 | 900篇 |
农作物 | 138篇 |
水产渔业 | 235篇 |
畜牧兽医 | 1688篇 |
园艺 | 1312篇 |
植物保护 | 36篇 |
出版年
2024年 | 14篇 |
2023年 | 43篇 |
2022年 | 156篇 |
2021年 | 176篇 |
2020年 | 167篇 |
2019年 | 237篇 |
2018年 | 135篇 |
2017年 | 198篇 |
2016年 | 223篇 |
2015年 | 222篇 |
2014年 | 245篇 |
2013年 | 289篇 |
2012年 | 330篇 |
2011年 | 325篇 |
2010年 | 165篇 |
2009年 | 173篇 |
2008年 | 192篇 |
2007年 | 295篇 |
2006年 | 151篇 |
2005年 | 110篇 |
2004年 | 151篇 |
2003年 | 106篇 |
2002年 | 84篇 |
2001年 | 68篇 |
2000年 | 73篇 |
1999年 | 34篇 |
1998年 | 27篇 |
1997年 | 21篇 |
1996年 | 24篇 |
1995年 | 21篇 |
1994年 | 28篇 |
1993年 | 31篇 |
1992年 | 17篇 |
1991年 | 9篇 |
1990年 | 13篇 |
1989年 | 14篇 |
1988年 | 15篇 |
1987年 | 10篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1973年 | 1篇 |
1956年 | 8篇 |
排序方式: 共有4629条查询结果,搜索用时 15 毫秒
81.
82.
Sakamoto K Yano T Kobayashi T Hagino A Aso H Obara Y 《Domestic animal endocrinology》2007,32(4):260-272
Growth hormone (GH) plays a specific role to inhibit apoptosis in the bovine mammary gland through the insulin-like growth factor (IGF)-I system, however, the mechanism of GH action is poorly understood. In this study, we show that GH dramatically inhibits the expression of IGFBP-5, and GH along with IGF-I enhanced the phosphorylation of Akt through the reduction of IGF binding protein (IGFBP)-5. To determine how GH affects Akt through IGF-I in bovine mammary epithelial cells (BMECs), we examined the phosphorylation of Akt in GH treated BMECs and found that IGF-I induced phosphorylation of Akt was significantly enhanced by the treatment with GH. We demonstrated that GH reduces mRNA and protein expression of IGFBP-5 in BMECs, but it does not affect the expression of IGFBP-3. To determine that the enhanced effect of the Akt phosphorylation by the treatment of GH is due to the inhibition of the expression of IGFBP-5, we examined the effect of IGFBP-3 and -5 on the phosphorylation of Akt through IGF-I in the GH-treated BMECs. The phosphorylation of Akt was inhibited in a dose-dependent manner when IGFBP-5 was added at varying concentrations and was also inhibited in the presence of IGFBP-3. The results of this study suggest that GH plays an important role on mammary gland involution in bovine mammary epithelial cells. 相似文献
83.
为对鼠源细胞进行鉴别检测,以鼠线粒体16S rRNA基因序列为靶位点设计特异性引物及探针,建立实时荧光定量PCR检测方法,并评价该方法的特异性及敏感性。结果显示,所建立的检测方法特异性好,针对鼠源细胞基因组荧光定量PCR扩增曲线良好,其他物种来源细胞基因组及生物制品原辅材料未出现特异性扩增曲线;敏感性高,基因拷贝数检出限度为45.3拷贝。本试验建立的荧光定量PCR检测方法能够有效地对鼠源细胞进行快速检测,为细胞质量控制提供了有效方法。 相似文献
84.
XIANG Li-jun WANG Bo-chu DUAN Chuan-ren LIU Liu WANG Qing-hongnistry of Education College of Bioengineering Chongqing University Chongqing Chin 《保鲜与加工》2004,(10):71-74
The paper researches on the degradation of LAS (Linear Alkyl benzene Sulphonate) by combining AS1.860 immobilized with low intensity ultrasonic irradiation. The paper also discusses the influence of pH, rotary velocity, LAS concentration and the condition of low intensity ultrasonic irradiation on the degradation of LAS, the degradation rate of LAS is the main index of our experiment, the results of orthogonal test shows that low ultrasonic irradiation can increase the metabolizing of microorganism cells and facilitate the biodegradation of immobilized cells to LAS, here we research the degradation condition of 50mg/L LAS simulation wastewater with low ultrasonic irradiation, and the results show that the influence is obvious, the optimization degradation rate is about 83%, nine point five percent higher than that of the immobilized cells without ultrasonic irradiation. 相似文献
85.
内毒素对肠黏膜微血管内皮细胞内分泌一氧化氮和内皮素的影响 总被引:1,自引:1,他引:1
目的是研究内毒素(lipopolysaccharide; LPS)导致肠黏膜微血管内皮细胞损伤的作用机制。将所培养的肠黏膜微血管内皮细胞分为空白对照组和LPS组,每组按时间分为4个亚组:3h、6h、9h、12h。空白对照组加维持培养液,LPS组加1μg/ml LPS培养液,在CO2培养箱内静置培养。结果表明,一氧化氮(NO)分泌明显升高,3h后达到高峰,随后逐渐降低,而内皮素(endothelin; ET)分泌急剧升高,9h后达到高峰,随后又开始逐渐降低,但一直保持在较高的水平。所以引起了NO和ET的升高可能是LPS导致肠黏膜微血管内皮细胞的损伤机制。 相似文献
86.
87.
Mast cells are important cells for the innate immunity that reside in tissues including adipose tissue and are involved in various physiological and pathological processes by producing a range of biological mediators. Adipose tissue not only acts as an energy depot and regulator of energy homeostasis that can deposit excess energy and dissipate energy through heat, but also is an active endocrine organ capable of producing hormones and adipokines. The dysfunction of adipose tissue is highly correlated with metabolic disorders, such as obesity, type 2 diabetes mellitus and so on. This review focuses on the physiological and pathological roles of mast cells in adipose tissue. 相似文献
88.
大银鱼卵细胞粘丝扫描电镜观察 总被引:1,自引:0,他引:1
用扫描电镜观察了大银鱼的第Ⅲ时相,第Ⅳ时相,第Ⅴ时相卵母细胞和受精卵的卵细胞粘丝的外部形态特征和它们的粘性,并讨论了卵细胞粘丝的功能。 相似文献
89.
90.