亚麻籽对肉鸡生长、屠宰性能及肉品质的影响

文章旨在研究日粮添加不同水平亚麻籽对肉鸡生长、屠宰性能及肉品质的影响。试验选择1日龄AA肉鸡512只,随机分为4组,每组4个重复,每个重复32只鸡。试验以玉米-豆粕为基础日粮,各组日粮分别在基础日粮中添加0、5%、10%和15%亚麻籽,试验分为两个阶段,共进行42 d。15%亚麻籽组比对照组日增重显著降低10.27%(P <0.05),同时试验后3周体重显著降低7.28%(P <0.05)。随着日粮亚麻籽添加水平的升高,整个试验期肉鸡日增重显著降低(P <0.05)。对照组和5%亚麻籽组较10%和15%亚麻籽组显著降低了0~3周肉鸡料肉比(P <0.05),而对照组较5%和15%亚麻籽组显著降低了4~6周肉鸡料肉比(P <0.05),同时对照组较所有亚麻籽组显著降低了试验全期料肉比(P <0.05)。5%和15%亚麻籽组较对照组显著降低了肉鸡蛋白质和能量利用效率(P <0.05)。与其他各组相比,15%亚麻籽组显著降低了肉鸡胴体重(P <0.05);15%亚麻籽组较对照组和10%亚麻籽组显著降低了胸肌相对重量(P <0.05),而对照组较5%和15%亚麻籽组显著降低了腿肌相对重量(P <0.05)。随着日粮亚麻籽添加水平的升高,胸肌和腿肌亚麻酸含量显著升高(P <0.05),但对照组与5%亚麻籽组对腿肌亚麻酸含量无显著影响(P> 0.05),10%和15%亚麻籽组较对照组显著提高了胸肌和腿肌亚油酸含量(P <0.05)。在本试验条件下,日粮添加15%亚麻籽使肉鸡日增重降低8.2%,但日粮添加亚麻籽可以提高胸肌和腿肌亚油酸和亚麻酸含量,因此,日粮中适宜的亚麻籽添加水平为10%。     

Effects of Supplementing a Basal Diet of Chloris gayana Hay with One of Three Protein-rich Legume Hays of Cassia rotundifolia,Lablab purpureus and Macroptilium atropurpureum Forage on Some Nutritional Parameters in Goats

Growth and digestibility experiments were conducted on growing East African type goats offered Chloris gayana hay supplemented with one of three high-protein (119–128 g CP/kg DM) legume hays, Cassia rotundifolia (cassia), Lablab purpureus (lablab) or Macroptilium atropurpureum (siratro), and crushed maize to investigate the feed intake, digestibility, growth and urinary excretion of purine derivatives. Goats in the supplemented groups had higher total dry matter and nitrogen intakes and higher N retention and body mass gains than unsupplemented counterparts. The digestibility of dry matter, organic matter and neutral detergent fibre were increased by protein supplementation. Animals on supplemented diets had higher fractional outflow rates of particulate matter from the rumen. The production of protein by ruminal microbes and the efficiency of microbial N production were increased by supplementation. It was concluded that a mixture of low-quality grass hay (61.9 CP/kg DM) and either cassia, lablab or siratro hay, and maize grain can provide a productive balanced diet for growing goats.     

转基因技术在畜牧兽医中的发展前景

通过简要叙述转基因动物研究的发展简史、研究现状和存在问题 ,对转基因动物育种、药物生物反应器、组织器官移植 ,以及基因治疗和免疫等前景进行了综述。在此基础上 ,从畜牧兽医角度出发提出了追踪发展趋势的几点思考。     

甲醛处理对豆饼干物质和蛋白质瘤胃降解率及胃蛋白酶消化率的影响

豆饼用２ｇ／ｋｇＤＭ甲醛处理后,１０ｈ瘤胃干物质（ＤＭ）降解率从８７．１９％下降到豆饼含水量为１４％和６０．９３％和含水量１８％时的５６．２９％,粗蛋白降解率从８７．６９％分别下降到４８．３６％和４３．４３％,随着甲醛用量的增加,干物质的粗蛋白的降解率进一步下降,但下降幅度减少。在甲醛用量低于２ｇ／ｋｇＤＭ的情况下,甲醛用量对ＰＨ为４．５时的胃蛋白酶４ｈ和８ｈ干物质和粗蛋白消化率没有明显影响,但     

家蚕质型多角体病毒研究的若干新进展

家蚕质型多角体病毒(BmCPV)分为9个株系,可使20种昆虫感染发病。本文阐述了近年来BmCPV在交叉感染、结构、基因组、结合蛋白与功能等方面的一些研究新进展。据报道BmCPV-1的两个毒株(H株与I株)的基因组全序列已被测定公布,由10个节段的双链RNA构成;众多研究认为BmCPV具有5种结构蛋白(VP1-5),推测VP1为依赖于RNA的RNA聚合酶,VP2、VP5分别为核衣壳蛋白、外壳蛋白,VP3、VP4各为三磷酸核苷酸结合蛋白、非特异性的核酸结合的关键蛋白,5种结构蛋白在BmCPV病毒复制包装过程中发挥着不同的功能。     

禽白血病病毒ELISA检测方法的建立与初步应用

本研究以在毕赤酵母系统中表达并纯化的p27重组蛋白为包被抗原,HRP标记的兔抗鸡IgY为酶标二抗,建立了一种快速有效的禽蛋白血病病毒ELISA检测方法,并对其反应条件进行优化。结果显示,该方法具有良好的特异性和重复性;与市售商品试剂盒相比,符合率为96.92%,且更为敏感。应用建立的ELISA方法检测来自吉林省内鸡场314份疑似样品,阳性检出率为75.15%。本研究为禽白血病病毒的检测与诊断提供了一种简便、有效的检测方法。     

消化道上皮细胞中乳酸杆菌黏附配体蛋白含量的变化

利用兔抗鸡乳酸杆菌黏附配体蛋白血清,以不连续活性-PAGE电泳技术和间接ELISA方法,对不同日龄、健康和患球虫病鸡消化道不同部位乳酸杆菌黏附配体蛋白含量进行了检测。结果表明,2日龄鸡嗉囊部位产生乳酸杆菌黏附配体蛋白成分,D450nm值为0.181;1日龄鸡小肠部位产生乳酸杆菌黏附配体蛋白成分,D450nm值为0.168;5日龄乳酸杆菌黏附配体蛋白成分达到稳定,D450nm值分别为0.200和0.123。健康鸡体内嗉囊与小肠部位乳酸杆菌黏附配体蛋白含量比患球虫病鸡明显增多,D450nm值分别为：嗉囊,0.143和0.132;小肠,0.148和0.134。     

Study on Nutritional Characteristics of Cottonseed Hull as a Feed of Sheep

In this paper through digestive and metabolic experiments studied the voluntary intake and apparent digestibility of cottonseed hulls (kernel-contained CH,kernel-free CH) and cornstalk on sheep to evaluate the nutrition value and digestion characteristics of cottonseed hulls.12 of adult Xinjiang merino rams were selected and divided into two groups (n=6 each group),and on the basis of 300 g/(head·day) of mixed concentrate,fed with kernel-free CH by 400 (group 1) or 800 (group 2) g/(head·day) respectively,to estimate the digestibility of CH by difference method;As well,another 18 of adult Xinjiang merino rams were selected and divided into three groups (n=6 each group),and on the basis of 300 g/(head·day) of mixed concentrate,cafeteria feeding with kernel-contained CH (group 3),kernel-free CH (group 4) or ground cornstalks (group 5) respectively,to study the effects of three diets on the voluntary intake,digestion and metabolism of sheep.The results showed that the apparent digestibility of the DM,OM,CP,cellulose,hemicellulose and energy of kernel-free CH by sheep were 46.71%,47.37%,-53.40%,66.60%,62.48% and 42.59%,respectively.There were no significant differences in the intake of DM,OM,cellulose,hemicellulose,energy and Ca between kernel-contained CH and kernel-free CH groups (P> 0.05);Intake of CP and P in kernel-contained CH group were extremely significantly higher than that in kernel-free CH group (P< 0.01);Intake of lignin in kernel-contained CH group was extremely significantly lower than that in kernel-free CH group (P< 0.01).There were no significant differences in the apparent digestibility of DM,OM and energy between kernel-contained CH and kernel-free CH groups (P> 0.05).The apparent digestibility of cellulose and hemicellulose in kernel-free CH group were significantly and extremely significantly higher than that in kernel-contained CH group (P< 0.05;P< 0.01),respectively.The apparent digestibility of CP in kernel-contained CH group was extremely significantly higher than that in kernel-free CH group (P< 0.01).The apparent digestibility of DM,OM,CP,cellulose and energy in ground cornstalks group were extremely significantly higher than that in kernel-contained CH and kernel-free CH groups (P< 0.01),however,the apparent digestibility of Ca and P were lower than that in kernel-contained CH and kernel-free CH groups (P> 0.05).Intake and retention of nitrogen in kernel-contained CH group were extremely significantly higher than that in kernel-free CH group (P< 0.01);The was no significant difference in the retention of nitrogen between kernel-free diet group and cornstalk diet group (P> 0.05).It was concluded that CH was a kind of special forage with larger intake,higher digestibility on the fiber substances and energy,but with a serious obstruction on protein digestion;The nutritional value between kernel-free diet and cornstalk diet was almost equal,and with lower nitrogen digestibility on kernel-free diet.     

An investigation into reduced milk production following dietary alteration on an Irish dairy farm

A nutritional evaluation of an Irish dairy herd indicated gross overfeeding of late lactation cows, over-conditioning of cows at parturition and a high rate of body condition loss in early lactation. Metabolisable-energy based nutritional modelling software was used to guide recommended dietary changes to prevent excessive condition gain in late lactation. Immediately following the implementation of the changes there was an unexpected reduction in performance affecting both milk yield and protein concentration. An investigation into the poor performance revealed underestimation of peak milk yield; over-estimation of maize silage quality; a large difference in the concentrate being fed compared to the concentrate recommended, and failure of the blend of concentrate ingredients to maintain the intended proportions in the in-parlour feeding system. The estimated maximum cumulative effect of these errors was to cause undersupply of energy and protein in the recommended diet of 16% and 3% respectively to cows in early lactation. Use of a net-energy nutritional model would have indicated a requirement for a higher energy supply in this case. This report highlights the challenges in obtaining accurate on-farm data for use in dairy cow nutritional models.