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61.
为揭示多花黑麦草(Lolium multiflorum Lamk)对不同钙磷环境的适应机制,设置3个不同钙磷比(1∶1、2∶1、5∶1),每个钙磷比下设置两种钙磷浓度,以不添加钙和磷的营养液处理为对照,探讨不同钙磷水平下多花黑麦草形态和生理指标的变化;并利用隶属函数综合评价法来筛选适宜多花黑麦草生长的钙磷配比及浓度。结果表明:①Ca/P比为1∶1时,随Ca2+、P浓度的增加,株高、叶宽、光合色素和脯氨酸(proline, Pro)含量均呈下降趋势,丙二醛(malondialdehyde, MDA)和超氧化物歧化酶(superoxide dismutase, SOD)活性呈升高趋势;②Ca/P比为2∶1时,随Ca2+、P的增加,株高、叶宽存在一定的下降,叶绿素含量、MDA含量和SOD活性差异不显著,类胡萝卜素和Pro含量升高;③Ca/P比为5∶1时,随Ca2+、P浓度的增加,株高、叶宽、光合色素含量升高,MDA含量下降,Pro含量和SOD活性变化不明显;④P浓度相同时,随着Ca2+浓度的增加,叶绿素、类胡萝卜素含量和SOD活性呈降低趋势;⑤通过隶属函数综合评价,多花黑麦草适宜生长的Ca、P浓度分别为50和10 mg·kg-1。以上结果为多花黑麦草的栽培种植和钙磷响应机理研究提供理论依据。 相似文献
62.
Magdalena Mititelu Gabriela Stanciu Doina Drgnescu Ana Corina Ioni Sorinel Marius Neacu Mihaela Dinu Raluca-Ioana Stefan-van Staden Elena Moroan 《Marine drugs》2022,20(1)
(1) Background: The mussel (Mytilus edulis, Mytilus galloprovincialis) is the most widespread lamellibranch mollusk, being fished on all coasts of the European seas. Mussels are also widely grown in Japan, China, and Spain, especially for food purposes. This paper shows an original technique for mussel shell processing for preparation of calcium salts, such as calcium levulinate. This process involves synthesis of calcium levulinate by treatment of Mytilus galloprovincialis shells with levulinic acid. The advantage of mussel shell utilization results in more straightforward qualitative composition. Thus, the weight of the mineral component lies with calcium carbonate, which can be used for extraction of pharmaceutical preparations. (2) Methods: Shell powder was first deproteinized by calcination, then the mineral part was treated with levulinic acid. The problem of shells generally resulting from the industrialization of marine molluscs creates enough shortcomings, if one only mentions storage and handling. One of the solutions proposed by us is the capitalization of calcium from shells in the pharmaceutical industry. (3) Results: The toxicity of calcium levulinate synthesized from the mussel shells was evaluated by the method known in the scientific literature as the Constantinescu phytobiological method (using wheat kernels, Triticum vulgare Mill). Acute toxicity of calcium levulinate was evaluated; the experiments showed the low toxicity of calcium levulinate. (4) Conclusion: The experimental results highlighted calcium as the predominant element in the composition of mussel shells, which strengthens the argument of capitalizing the shells as an important natural source of calcium. 相似文献
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Jean‐Pierre Cuif Yannicke Dauphin Gilles Luquet Oulfa Belhadj Stphan Rouzire Murielle Salom Marine Cotte Andrea Somogyi Kadda Medjoubi Cedrik Lo Denis Saulnier 《Aquaculture Research》2020,51(2):506-522
Cultivated pearls frequently exhibit morphological irregularities making obvious that mineral deposition was irregularly distributed onto nucleus surface. Taking advantage of experimental cultivations with short durations (from 10 days to few months), these irregular deposits predating occurrence of the nacre were investigated in Polynesian pearls by biochemical characterizations and a series of physical methods. Diversity in the resulting data suggests that various in‐depth alterations of the biomineralization mechanism may have occurred during the grafting process, leading to diversity in the biochemical pathways to nacreous deposition. This allows a precise discussion of current views about pearl formation. The “reversed shell theory” is formally disproved through point to point comparison with development of the shell growing edge. Similarity of pearl formation with “regeneration” or “shell repair” is also discussed, emphasizing the differences between these concepts. 相似文献
66.
Gene expression in black tiger prawns (Penaeus monodon) was studied following intra-muscular injection of CMVGal plasmid into the second abdominal segment. We used an in situ staining technique to detect -gal expression in one- and three-month-old injected prawns. We found that only one of the three-month-old prawns expressed the marker gene (2 days after injection), and the site of expression was confined to the sixth abdominal segment away from the injection site. We repeated the experiment on a new batch of three-month-old prawns, but using fluorometric determination technique. This time we found that -gal expression was detected (6/42) at the site of injection after 2, 7, and 14 days. In two other test samples, transgene expression was detected in the sixth abdominal segment only, further confirming the possibility of injected DNA dispersal. The results of the study also suggest that direct gene transfer is a feasible technique in black tiger prawns. 相似文献
67.
The Effects of Light Salting on Physicochemical Characteristics of Frozen Cod (Gadus morhua) Fillets
《Journal Of Aquatic Food Product Technology》2013,22(3-4):287-301
Abstract The effects of light salting by brine injection and brine immersion on physicochemical and textural properties of cod fillets were evaluated. Light salting significantly increased yield and water holding capacity. Adding brine injection to the process can be used to shorten the process time needed to obtain the desired salt content and increase yield. The effects on chemical composition were most significant in salt content, but water and protein content were also affected. Water content decreased from the tail to head direction of the fillets, both in unsalted and light salted fillets. Changes in protein content were in opposite proportion to changes in water content. This could be seen in the fresh fish and also after freezing, both in unsalted and light salted fillets. Texture of the fish was not significantly affected by salting and freezing. 相似文献
68.
柴油机高压共轨系统喷油量和喷油规律测量方法概述 总被引:1,自引:0,他引:1
对目前几种常用的测量柴油机高压共轨系统喷油量和喷油规律的方法、原理和装置进行了介绍与分析。 相似文献
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70.
钙提高水稻幼苗抗旱性的研究 总被引:32,自引:1,他引:32
将水稻种子用CaCl2溶液浸种18 h后催芽、播种,培养13 d后进行渗透胁迫处理。Ca2+浸种的水稻幼苗在-0.5 MPa的渗透胁迫下地上部相对含水量提高,质膜相对透性降低,表明提高了抗旱性。异丙嗪(CPZ)和CPZ + Ca2+浸种亦提高了水稻抗旱性。渗透胁迫1 d后,Ca2+浸种的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和抗坏血酸(AsA)、还原型谷胱甘肽(GSH)含量均提高。实验结果表明,Ca2+处理提高膜脂过氧化的保护能力和膜的稳定性可能是其提高水稻幼苗抗旱性的原因。 相似文献