Fat globule membrane of sow milk as a target for adhesion of K88-positive Escherichia coli

Membrane adhesion of K88-positive Escherichia coli was studied on intestinal brush-border membranes on 237 Finnish Landrace pigs. Forty-one per cent of the brush-border membrane preparations aggregated E. coli (positive adhesion). Similar dualism of adherence/nonadherence was observed on sow milk fat globule membranes. Washed milk fat globules (washed cream) can be used as a convenient source of material for adhesion studies. Bacterial adherence on to milk fat globules is evident as agglutination of the globules (dark-field microscopy). By this procedure the sows can be typed according to their receptor phenotype. This simple principle of fat globule agglutination due to receptors for K88-positive E. coli might be complicated by SigA-mediated bacterial adherence. Fat globule membranes were shown to contain SigA, which may act as a mediator of bacterial adherence onto fat globules. The significance of this adhesive property of milk fat globule might be to provide alternative receptors for E. coli thus preventing bacterial adhesion on to gastro-intestinal epithelium of the offspring. Sow milk fat globules can be used for typing E. coli for membrane adhesiveness. The adhesiveness of the strains showed a good correlation with the presence of the K88 antigen, as well as the hydrophobicity of the bacterial strain as determined by an association on Phenyl-Sepharose beads.     

Expression of intercellular adhesion molecule in lung tissues of experimental acute lung injury and the effect of rhubarb

AIM:To approach the relationship between the expression of intercellular adhesion (ICAM-1 mRNA) and acute lung injury (ALI) as well as the mechanisms of rhubarb in the prevention and treatment of the lung injury. METHODS:ALI animal model was performed by Lipopolysaccharide (LPS). The rats were divided into 4 groups: LPS group, control group, rhubarb+LPS group and dexamethasone+LPS group. Histopathological examination and biological markers were measured for the lung specimens. Molecular hybridization method was used to determine the expression of ICAM-1 mRNA. RESULTS:The ICAM-1 mRNA expression in the lung tissues of LPS group significantly increased compared with control group (P＜0.01), rhubarb and dexamethasone had the action of decreasing the ICAM-1 mRNA expression (P＜0.05, P＜0.01); pathologic changes and the biological markers of ALI significantly decreased or ameliorated. CONCLUSION:The increase in the expression of ICAM-1 mRNA in the lung tissues of ALI is involved in the formation of ALI. Rhubarb and dexamethasone can ameliorate the lung damage, mechanism of which may be related to the inhibition of ICAM-1 mRNA expression.     

Effect of Busui Shengxue Granule (补髓生血颗粒) on Chronic Aplastic Anemia Patients' Hematopoietic Adhesion Molecule VLA-6/CD49f and Its Ligand Laminin

Objective:To observe the effect of Busui Shengxue Granule (补髓生血颗粒Herbal granule for replenishing marrow to produce blood) on chronic aplastic anemia (CAA) patients' integrin a 6 (VLA-6/CD49f) and...     

Expression of EPCAM,CD44 and CD24 in gastric cancer tissues and its clinical significance

AIM: To evaluate the relationship between epithelial cell adhesion molecule (EPCAM)/cluster of differentiation 44 (CD44)/cluster of differentiation 24 (CD24) expression and the clinicopathological characteristics/prognosis in 95 gastric cancer patients. METHODS: The expression levels of EPCAM, CD44 and CD24 were detected using the two-step method of immunohistochemistry in 95 gastric cancer patients who underwent surgical excision and were pathologically diagnosed as gastric cancer. RESULTS: There were 56 EPCAM-positive patients (58.95%), 41 CD44-positive patients (43.16%) and 56 CD24-positive patients (58.95%). Thirty patients were both EPCAM and CD44 positive (31.58%), 45 patients were both EPCAM and CD24 positive (47.37%), 32 patients were both CD44 and CD24 positive (33.68%), and 25 patients were EPCAM, CD44 and CD24 positive (26.32%). EPCAM expression was correlated with age, depth of tumor infiltration and WHO histological classification. CD44 expression was correlated with BORRMANN and WHO histological classification as well as CEA value. CD24 expression was correlated with the depth of infiltration, location of the tumor, WHO histological classification and viscera invasion. All positive expression of EPCAM, CD44 and CD24 was correlated with the depth of infiltration, location of the tumor and WHO histological classification (P<0.05). The difference of survival rate between EPCAM positive group and negative group was observed, and the CD44 positive group and negative group had the same result (P<0.05 and P<0.01, respectively). The difference of survival rate between EPCAM⁺CD44⁺CD24⁺ group and EPCAM^-CD44^-CD24^- group was statistically significant (P<0.05). The difference of survival rate between EPCAM^-CD44⁺CD24⁺ group and EPCAM^-CD44^-CD24^- group was also significant (P<0.05). CONCLUSION: The positive rates of EPCAM, CD44 and CD24 expression are high in gastric cancer tissues and these 3 proteins can be used as primary screening targets.     

消化道上皮细胞中乳酸杆菌黏附配体蛋白含量的变化

利用兔抗鸡乳酸杆菌黏附配体蛋白血清,以不连续活性-PAGE电泳技术和间接ELISA方法,对不同日龄、健康和患球虫病鸡消化道不同部位乳酸杆菌黏附配体蛋白含量进行了检测。结果表明,2日龄鸡嗉囊部位产生乳酸杆菌黏附配体蛋白成分,D450nm值为0.181;1日龄鸡小肠部位产生乳酸杆菌黏附配体蛋白成分,D450nm值为0.168;5日龄乳酸杆菌黏附配体蛋白成分达到稳定,D450nm值分别为0.200和0.123。健康鸡体内嗉囊与小肠部位乳酸杆菌黏附配体蛋白含量比患球虫病鸡明显增多,D450nm值分别为：嗉囊,0.143和0.132;小肠,0.148和0.134。     

血清可溶性细胞间粘附分子在大肠癌患者组织中的检测及其意义

目的:探讨血清可溶性细胞间粘附分子1(sICAM-1)的检测在大肠癌患者中的临床意义。方法:采用双抗体夹心ELISA法检测50例大肠癌、30例大肠良性病变及30例健康体检者血清sICAM-1水平,并比较大肠癌患者术前、术后,转移、无转移sICAM-1水平变化。结果:大肠癌患者血清sICAM-1水平明显高于良性病变患者及正常人(P<0.01);大肠癌患者术后血清sICAM-1水平较术前明显下降(P<0.01);有转移的大肠癌患者血清sICAM-1水平高于无转移的患者(P<0.01)。结论:血清sICAM-1水平与大肠癌的发生、发展密切相关,可作为大肠癌新的检测指标之一。     

草鱼源致病性维氏气单胞菌的鉴定及其黏附特性分析

采用表型鉴定与细菌16S rRNA基因序列分析对从患病草鱼体内分离的病原菌株16-1进行分类鉴定,综合该菌株的表型特征与16S rRNA基因序列,确定其为维氏气单胞菌。随后,对该菌株的细胞黏附特性及其携带的黏附素基因进行检测与分析。结果显示,分离菌株能以聚集性方式黏附于鲤鱼上皮瘤细胞（EPC）周围,平均黏附菌数随共孵育时间延长而增加,90 min达到峰值,说明该分离株具有良好的细胞黏附性。黏附因子检测表明,该菌株同时携带ompAI、ompAII和ahl13种黏附素基因,这些黏附素基因在分离菌株与不同来源参考株之间的核苷酸序列和氨基酸序列同源性分别介于95.8%～99.5%和95.0%～100%（ompAI）,96.3%～99.1%和97.9%～100.0%（ompAII）,78.6%～99.6%和75.5%～99.4% （aha1）,说明所携带的ompAI和ompAII黏附素基因在不同来源的维氏气单胞菌之间具有较高的保守性。     

水性聚氨酯漆料干燥速率及漆膜性能影响规律研究

以4种水性聚氨酯漆料为研究对象,系统研究了漆料和基材类型、涂饰工艺对水性漆表干、实干时间和漆膜硬度、附着力及耐磨性的影响规律。结果表明:漆料的表实干时间及漆膜性能很大程度受成膜树脂本身性能影响;基材影响漆料表实干时间、漆膜硬度和附着力,对漆膜耐磨性影响不大,漆料涂饰在樱桃木和水曲柳上的干燥速率最快,在樱桃木、沙比利和水曲柳上的硬度最佳,达2 H,在樱桃木、沙比利和红橡上的附着力最佳,达0级;涂饰工艺对漆料的表干、实干时间以及漆膜硬度、附着力、耐磨性的影响都不明显,3种工艺涂饰后漆膜硬度和附着力分别为2 H和0级,漆膜耐磨性差别不大。     

基于活动苗盘的钵苗脱盘基质损失的试验研究

针对穴盘苗脱盘过程中基质损失导致取苗成功率降低的问题,以活动苗盘所育油菜钵苗为研究对象,选取苗盘锥角角度(7°、9°、11°)、基质压实度(1.0、1.1、1.2)、基质含水率(55%、60%、66%)为试验因素,以苗钵基质损失率为试验指标值,进行正交试验,研究苗钵脱盘过程中各因素对苗钵基质损失率的影响。结果表明:穴盘苗脱盘过程中,苗钵基质平均损失率为3.09%;苗盘锥角角度和基质压实度对基质损失率的影响极显著,基质含水率对基质损失率的影响显著;各因素对苗钵基质损失率影响大小依次为基质压实度、苗盘锥角角度、基质含水率;当苗盘锥角角度为11°、基质压实度为1.2、基质含水率为66%时,苗钵基质损失率最小,为2.62%。