鲫、草鱼外周血中淋巴细胞和单核细胞对鲤血管内皮细胞的粘附

借鉴哺乳动物内皮细胞体外培养技术，结合鱼类细胞自身特点，纯化培养鲤血管内皮细胞并传至3代，以此作为粘附材料。应用淋巴细胞分离液离心技术并结合玻璃粘附法分离纯化鲫、草鱼外周血中淋巴细胞、单核细胞，并与上述3代内皮细胞进行免疫粘附试验，同时进行免疫粘附动力学观察。结果显示，鲫、草鱼两类细胞对内皮细胞的粘附率分别为0.09±0.013，0.20±0.018；0.11±0.015，0.21±0.023。表明鲤科鱼类不同种的同类细胞粘附率差异不大，而同种不同类细胞则差异显著。动力学观察分析表明，淋巴细胞粘附较快，60min进入平台期；单核细胞粘附慢，120min进入平台期。初步证明鱼类内皮细胞具有免疫介导作用。     

Effects of Pinus massoniana bark extract on the adhesion and migration capabilities of HeLa cells

Pinus massoniana Lamb is a Chinese red pine species used in traditional medicine for the treatment of a variety of human health disorders. Recent studies have shown that P. massoniana bark extract (PMBE) has an anti-proliferation effect on cancer cells. However, it is not clear if PMBE affects cancer cell migration and/or invasion. We tested the effect of PMBE, which has B-type procyanidin as its main constituent, on the adhesion and migration capabilities of HeLa cells, a human cervical cancer cell line, cultured in vitro. Our results showed that PMBE has no significant effect on the adhesion capability of HeLa cells, but strongly inhibits their migration. This finding suggests that PMBE could be a potential therapeutic agent for metastatic cancer.     

Effect of different droplet size on the knockdown efficacy of directly sprayed insecticides

BACKGROUND: Although insecticidal aerosols have been widely accepted for household use, the discharged amount should be maintained at minimum levels because they contain volatile organic compounds. Hence, it would be valuable to develop a technique whereby insecticide droplets adhere efficiently to an insect's body. The present study was undertaken in order to clarify how differences in the mode of adhesion to the insect body influence the knockdown effect. RESULTS: When the discharged volume of droplets with different diameters was the same, the adhesion volume of larger droplets was twice that of smaller droplets, resulting in a higher insect knockdown. In contrast, when the adhesion volume of the two droplet types was the same, a greater number of smaller droplets than larger droplets adhered, and the smaller droplets caused higher insect knockdown. The knockdown effect of both droplet types was lowered when the mesothoracic spiracles of cockroaches were blocked; however, the effect of larger droplets was lowered to a lesser degree. CONCLUSION: The results suggest that, the probability of adhesion to the more susceptible regions of an insect's body, i.e., areas surrounding the mesothoracic spiracles, was improved when a greater number of smaller droplets were adhered, resulting in higher knockdown. Copyright © 2011 Society of Chemical Industry     

纸浆抄造性能的评价方法

根据 Mardon剥离方程 ,提出用纸浆湿纸幅累积破裂功与粘附功的比值 S/A来评价纸浆的抄造性能 .利用湿纸幅 S/A值与干度的变化曲线可以看出湿纸幅干度对其抄造性能的影响     

重塑黏土圆锥指数和抗剪强度的关系

为了定量评价轮式车辆在软黏土条件的附着、牵引及通过性能并建立软黏土的圆锥指数与抗剪强度关系,采用非标准普式击实法制备黏土试样,分别通过微型贯入试验与直剪试验得到重塑黏土的圆锥指数与抗剪强度,发现23%含水率是二者变化趋势的分界点,重塑土样的含水率低于该值时其圆锥指数与抗剪强度都随含水率呈二次多项式关系变化,而在高于该临界含水率时二者都随含水率呈线性关系变化。同样,在23%含水率以下圆锥指数与抗剪强度线性相关,而当含水率高于临界点后该线性关系消失。抛开含水率变化的影响,临界含水率以下的重塑样圆锥指数与抗剪强度关系仍然总体呈线性相关关系。     

Effects of ambroxol combined with low-dose heparin on production of ICAM-1 and E,P-selectin in acute lung injury

AIM: To study the production of intercellular adhesion molecule-1(ICAM-1), E-selectin and P-selectin in serum, lung tissues and bronchoalveolar lavage fluid(BALF)of acute lung injury(ALI) model and to observe the effects of ambroxol combined with low-dose heparin on the changes of the 3 factors above.METHODS: Twenty-four healthy rabbits were randomly divided into 3 groups: normal saline control group (NC), oleic acid injury group (OA), ambroxol+ heparin treatment group (AH). The rabbit ALI model was induced by oleic acid injection through auricular vein. Partial pressure of O₂ in artery(PaO₂) was analyzed.The concentrations of ICAM-1 and E-selectin were detected by ELISA.The apoptosis index(AI) was measured by TUNEL method.The expression of P-selectin was determined by immunohistochemical method.The ultrastructural changes of the lung tissues were observed under electron microscope, and the lung wet/dry ratio(W/D) was calculated.RESULTS: PaO₂ in AH group and OA group was significantly lower (P<0.01) than that in NC group, and PaO₂ in AH group was significantly higher than that in OA group (P<0.01). The concentrations of ICAM-1 and E-selectin in serum, lung tissues and BALF, and AI and W/D in lung tissues in AH group were higher (P<0.05 or P<0.01) than those in NC group, and was lower than those in OA group (P<0.05 or P<0.01). In NC group, no significant change of the above parameters at all time points was observed (P>0.05). In OA group, PaO₂ was significantly decreased (P<0.01) with the pathological process developed, and the concentrations of ICAM-1 and E-selectin were significantly increased. In AH group, PaO₂ was decreased (P<0.05),and the concentrations of ICAM-1 and E-selectin were increased with the process of ALI developed. The P-selectin expression in lung tissues of OA group was distributed mainly in inflammatory cells, capillary endothelial cells and plasma. From low to high levels, the order was NC group < AH group < OA group in the expression of P-selectin. The most obvious apoptosis was observed in OA group. No apoptosis or occasional positive cells were found in NC group. The apoptotic rate in AH group was significantly reduced compared with that in OA group.CONCLUSION: In ALI induced by OA, ICAM-1, E-selectin and P-selectin are significantly increased and are involved in the occurrence and development of ALI. Ambroxol combined with low-dose heparin reduces the levels of ICAM-1, E-selectin and P-selectin, the pulmonary edema and the lung injury, improves pulmonary functions, and plays an important role in the prevention and treatment of acute lung injury.     

影响木材粘接质量的因素及预防措施

论述了影响木材粘接质量的因素，提出了相应的预防措施。     

空穴负压现象的界面拉伸试验研究

应用类似于土壤粘附拉脱力试验的界面拉伸试验，证明了光滑表面的界面存在粘性物质时，如空气、水和黄油，界面介质在拉伸过程中将产生空穴负压现象，造成界面的粘附力增大；分析了空穴的形成、空穴膜的流动和破坏过程，并通过试验和高速摄影图片作了验证。     

磷脂酶C对血小板粘附性及表面膜GpIb的影响

采用旋转玻球法测定了磷脂酶C(PLC)经十二指肠给药后对家兔血小板粘附性的影响,同时利用竞争性酶联免疫法测定了PLC对人血小板表面膜糖蛋白Ib(GpIb)分子数的影响。结果表明,PLC冻干辅料及100和200U/kg的PLC对家兔血小板粘附率无明显影响,而400～1000U/kgPLC对血小板粘附率具有明显的影响。PLC冻干辅料对人血小板表面膜GpIb分子数没有明显影响,而每mL血浆(PRP)中加入0.5,1.0和2.0U的PLC对人血小板表面GpIb分子数可产生明显影响。     

双歧杆菌对病原菌粘附肉鸡肠粘液的影响

研究两歧双歧杆菌、禽大肠杆菌O78、大肠杆菌ATCC25922、鸡白痢沙门氏菌和鼠伤寒沙门氏菌与肉鸡不同肠段粘液糖蛋白的粘附性能，探讨两歧双歧杆菌对四种病原菌的粘附排斥作用。结果表明，在不同的肠道部位，两歧双歧杆菌、鸡白痢沙门氏菌和鼠伤寒沙门氏菌与肠粘液糖蛋白的粘附能力不同，而禽大肠杆菌O78、大肠杆菌ATCC25922在各肠段粘液上的粘附性能相近；在相同的肠道部位，所试菌与肠粘液糖蛋白的粘附能力有差异，其中两歧双歧杆菌的粘附作用最强；肠粘液糖蛋白经两歧双歧杆菌处理后，禽大肠杆菌O78、大肠杆菌ATCC25922和鸡白痢沙门氏菌的粘附作用受到抑制，而鼠伤寒沙门氏菌在十二指肠、空肠粘液上的粘附率未见下降。