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51.
The ontogenetic development of the digestive enzymes amylase, lipase, trypsin, and alkaline phosphatase and the effect of
starvation in miiuy croaker Miichthys miiuy larvae were studied. The activities of these enzymes were detected prior to exogenous feeding, but their developmental patterns
differed remarkably. Trypsin activity continuously increased from 2 days after hatching (dah), peaked on 20 dah, and decreased
to 25 dah at weaning. Alkaline phosphatase activity oscillated at low levels within a small range after the first feeding
on 3 dah. In contrast, amylase and lipase activities followed the general developmental pattern that has been characterized
in fish larvae, with a succession of increases or decreases. Amylase, lipase, and trypsin activities generally started to
increase or decrease at transitions from endogenous to exogenous feeding or diet changes, suggesting that these enzymatic
activities can be modulated by feeding modes. The activities of all the enzymes remained stable from 25 dah onwards, coinciding
with the formation of gastric glands and pyloric caecum. These results imply that specific activities of these enzymes underwent
changes due to morphological and physiological modifications or diet shift during larval development but that they became
stable after the development of the digestive organs and associated glands was fully completed and the organs/glands functioned.
Trypsin and alkaline phosphatase were more sensitive to starvation than amylase and lipase because delayed feeding up to 2 days
after mouth opening was able to adversely affect their activities. Enzyme activities did not significantly differ among feeding
groups during endogenous feeding; however, all activities were remarkably reduced when delayed feeding was within 3 days
after mouth opening. Initiation of larvae feeding should occur within 2 days after mouth opening so that good growth and survival
can be obtained in the culture. 相似文献
52.
L. A. Copeman C. C. Parrish J. A. Brown M. Harel 《Aquaculture (Amsterdam, Netherlands)》2002,210(1-4):285-304
The role of dietary ratios of docosahexaenoic acid (DHA, 22:6n−3), eicosapentaenoic acid (EPA, 20:5n−3) and arachidonic acid (AA, 20:4n−6) on early growth, survival, lipid composition, and pigmentation of yellowtail flounder was studied. Rotifers were enriched with lipid emulsions containing high DHA (43.3% of total fatty acids), DHA+EPA (37.4% and 14.2%, respectively), DHA+AA (36.0% and 8.9%), or a control emulsion containing only olive oil (no DHA, EPA, or AA). Larvae were fed differently enriched rotifers for 4 weeks post-hatch. At week 4, yellowtail larvae fed the high DHA diet were significantly larger (9.7±0.2 mm, P<0.05) and had higher survival (22.1±0.4%), while larvae fed the control diet were significantly smaller (7.3±0.2 mm, P<0.05) and showed lower survival (5.2±1.9%). Larval lipid class and fatty acid profiles differed significantly among treatments with larvae fed high polyunsaturated fatty acid (PUFA) diets having higher relative amounts of triacylglycerols (18–21% of total lipid) than larvae in the control diet (11%). Larval fatty acids reflected dietary levels of DHA, EPA and AA while larvae fed the control diet had reduced amounts of monounsaturated fatty acids (MUFA) and increased levels of PUFA relative to dietary levels. A strong relationship was observed between the DHA/EPA ratio in the diet and larval size (r2=0.75, P=0.005) and survival (r2=0.86, P=0.001). Following metamorphosis, the incidence of malpigmentation was higher in the DHA+AA diet (92%) than in all other treatments (50%). Results suggest that yellowtail larvae require a high level of dietary DHA for maximal growth and survival while diets containing elevated AA exert negative effects on larval pigmentation. 相似文献
53.
This study evaluated the lipid content and fatty acid (FA) profile of the hepatopancreas, ovaries and tail muscle of Lysmata amboinensis broodstock, as well as newly hatched larvae subjected to a period of starvation or feed from hatch to Zoea 2. The hepatopancreas had a high lipid content, confirming its role as a process and storage organ in L. amboinensis. Lipids were also a major component of ovarian dry weight, in agreement with reports on other crustaceans during maturation. The tail muscle, being a functional rather than a storage organ, contained low total lipids and was the tissue that closely resembled the FA profile of the newly hatched larvae. Saturated fatty acids (SFAs) and highly unsaturated fatty acids (HUFAs) were the most abundant components of the lipid profiles in broodstock and larvae. The HUFAs docosahexaenoic and eicosapentaenoic were preferentially retained during nutritional stress, confirming their importance for marine cleaner shrimp during early larval development. It appeared polyunsaturated fatty acid and HUFA requirements were met through the larval diet. The SFAs stearic and palmitic were abundant in adult tissues and larvae, whereas monounsaturated fatty acids may have been preferentially catabolized to meet energetic and metabolic larval requirements. 相似文献
54.
John Castell Tammy Blair Steven Neil Kenneth Howes Sarah Mercer John Reid Wilfred Young-Lai Brandi Gullison Philippe Dhert Patrick Sorgeloos 《Aquaculture International》2003,11(1-2):109-117
A feeding study was conducted in the winter 2001 to determine the effects of feeding rotifers (Brachionus plicatilis) enriched with various levels of essential fatty acids on the growth and survival of haddock larvae (Melanogrammus aeglefinus). Rotifer enrichment treatments were: 1) mixed algae, 2) high DHA (docosahexaenoic acid, 22:6n-3), 3) high DHA and EPA (eicosapentaenoic acid, 20:5n-3), and 4) DHA, EPA, and AA (arachidonic acid, 20:4n-6). Larvae were fed rotifers enriched with the different treatments from days 1 to 16 post-hatch. From day 17 until 25 all treatment groups were fed rotifers reared on mixed algae and then weaned onto the International Council for Exploration of the SEA (ICES) Standard Reference Weaning diet (http://allserv.rug.ac.t/aquaculture/rend/rend.htm) over a five day period. The experiment was terminated on day 41 post-hatch. The enrichment treatments affected the fatty acid composition of the rotifers and correlated with the accumulation of these fatty acids in the haddock larvae. However, no significant differences in larval growth or survival to 40 days post hatch were detected, suggesting that all treatments provided the minimal essential fatty acid requirements for haddock. 相似文献
55.
大黄鱼苗淀粉卵涡鞭虫病的诊断及防治方法 总被引:1,自引:0,他引:1
淀粉卵涡鞭虫是大黄鱼苗种培育危害最大的寄生虫之一.本文对大黄鱼苗淀粉卵涡鞭虫病发生的临床症状和防控方法进行了研究,除常规防治方法外,利用双氧水与高锰酸钾合用也可起到杀灭虫体,减少寄生数量的效果.同时,本文分析和比较了发生寄生虫病的育苗池水与正常池水的水质理化因子,为有效诊断和防治大黄鱼苗淀粉卵涡鞭虫病提供基础材料. 相似文献
56.
57.
Mahmoudreza Ovissipour Abdolmohammad Abedian Kenari Rajabmohammad Nazari Ali Motamedzadegan Barbara Rasco 《Aquaculture Research》2014,45(4):591-601
The effect of fish protein hydrolysate (FPH) on the growth, feed utilization, digestive enzymes, gut microflora and resistance to bacterial challenge in Persian sturgeon (Acipenser persicus) larvae during a 45‐day feeding trial has been investigated. Artificial diets containing different levels of tuna visceral protein hydrolysate (PH0, PH10, PH25 and PH50) and live foods (Artemia: 5 days, Daphnia: 40 days) were fed to Persian sturgeon larvae. Larvae fed on the PH10 and PH25 had growth, feed utilization, protein content and trypsin activity significantly better than PH0 and PH50 groups (P < 0.05). Condition factors, survival rate and pepsin activity were not significantly different (P > 0.05). Brush border membrane enzymes to cytosolic enzyme ratio revealed that maturation had been occurred in 41 days post hatch (dph) at moderate FPH levels. In 54 dph larvae, this ratio was not affected by the level of FPH in the diet. No significant differences were observed in the total number of gut microflora or in the resistance to Aeromonas hydrophila (P > 0.05), whereas lactic acid bacteria was significantly higher in PH50 (P < 0.05). The results of this study show that application of moderate levels of FPH in Persian sturgeon diets could enhance larvae performance due to the early maturation in intestinal. 相似文献
58.
59.
60.
Routine metabolic rates of green sturgeon (Acipenser medirostris) from hatching to 31 days post hatch (dph) were determined under normoxic conditions. During the endogenous feeding stage
that comprised period from hatching to 15 dph, the oxygen consumption rate (MO2, μg O2 larva−1 h−1) increased 5-fold before yolk reserves became exhausted and MO2 rates steady. The allometric relationships between MO2 and body mass had mass exponents greater than 1.0 (b=1.64±0.21) and equal to 1.0 (b=1.04±0.07) during the endogenous and exogenous feeding phases, respectively. The magnitude and changes of MO2 rates in green sturgeon larvae reflected their early ontogeny, especially during the endogenous feeding phase when the increase
in metabolic rates was associated with organogenesis, acquisition of organ functions, and the conversion of yolk sac into
new tissues.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献