全文获取类型
收费全文 | 6202篇 |
免费 | 327篇 |
国内免费 | 503篇 |
专业分类
林业 | 303篇 |
农学 | 501篇 |
基础科学 | 100篇 |
389篇 | |
综合类 | 2387篇 |
农作物 | 531篇 |
水产渔业 | 300篇 |
畜牧兽医 | 1435篇 |
园艺 | 779篇 |
植物保护 | 307篇 |
出版年
2024年 | 11篇 |
2023年 | 60篇 |
2022年 | 135篇 |
2021年 | 198篇 |
2020年 | 187篇 |
2019年 | 243篇 |
2018年 | 156篇 |
2017年 | 213篇 |
2016年 | 329篇 |
2015年 | 290篇 |
2014年 | 321篇 |
2013年 | 321篇 |
2012年 | 470篇 |
2011年 | 506篇 |
2010年 | 399篇 |
2009年 | 417篇 |
2008年 | 379篇 |
2007年 | 398篇 |
2006年 | 335篇 |
2005年 | 221篇 |
2004年 | 207篇 |
2003年 | 181篇 |
2002年 | 149篇 |
2001年 | 134篇 |
2000年 | 107篇 |
1999年 | 89篇 |
1998年 | 88篇 |
1997年 | 59篇 |
1996年 | 82篇 |
1995年 | 53篇 |
1994年 | 49篇 |
1993年 | 44篇 |
1992年 | 40篇 |
1991年 | 33篇 |
1990年 | 27篇 |
1989年 | 22篇 |
1988年 | 22篇 |
1987年 | 8篇 |
1986年 | 10篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 5篇 |
1980年 | 6篇 |
1979年 | 6篇 |
1978年 | 1篇 |
1962年 | 3篇 |
1956年 | 2篇 |
1955年 | 1篇 |
排序方式: 共有7032条查询结果,搜索用时 171 毫秒
21.
22.
23.
Although the molluscicide Frescon is a strong neurotoxin to the Lymnaea stagnalis central nervous system in vitro, it is probable that the exposure of the whole animal to this molluscicide fails to result in central nervous system abnormalities: Frescon does not appear to reach the brain in sufficient quantity to disrupt its normal activity. However, only those Frescon analogs found to be neurotoxic were molluscicidal, suggesting some related mode, if not site, of action. Frescon and its analogs may act by affecting excitable tissues other than the nervous system (e.g., the snail musculature) by altering certain functional and/or structural membrane properties. 相似文献
24.
AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO2-/NO3- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO2-/NO3- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway. 相似文献
25.
ZHOU Yan-bin YE Ren-gao XIE Can-mao XU Han-shi GUAN Wei-ming YANG Xiao YANG Nian-sheng 《园艺学报》2003,19(9):1221-1223
AIM:To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN).METHODS:Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed.RESULTS:The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively (r=0.5542, P<0.05;r=0.6279, P<0.05). CONCLUSIONS:The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN. 相似文献
26.
AIM:To investigate the effect of tea-polyphenols (TP) on the activation of NF-κB and the expression of TGF-β1 mRNA in THP-1 cells (a human acute monocytic leukemia cell line). METHODS:THP-1 cells were incubated with the different concentrations of TP, VLDL, LDL or ox-LDL. In the THP-1 cellls, the nuclear malposition rate of NF-κB was detected with immunohistochemistry technique, the positive index of the TGF-β1 mRNA expression was detected by hybridization in situ, and accumulation of total cholesterol (TC) in cells incubated with 0.4-40 μg/L TP was determined with oxidase assay. RESULTS:The nuclear malposition rate of NF-κB, the positive index of the TGF-β1 mRNA expression and TC in THP-1 cells incubated with 0.4-40 μg/L of TP were lower than those with 0 μg/L of TP in TP-V group, TP-L group and TP-O (P<0.05). The differences of these markers in THP-1 cells incubated with more than 40 μg/L TP in TP-V group, TP-L group and TP-O were not statistically significant, compared with TP-C group (P>0.05). CONCLUSION:TP inhibited the activation of NF-κB, the expression of TGF-β1 mRNA and the foam cell formation in the mono-macrophage. 相似文献
27.
28.
29.
6-BA在葡萄植株体内的运转和分配 总被引:10,自引:0,他引:10
以葡萄为试材,研究了葡萄根、茎、叶、果等器官对6-苄基腺嘌呤(6-BA)的吸收及它在植株内的运转和分配。结果表明,对1年生葡萄幼苗茎部引入或叶片涂抹6-BA,1h后,在植株所有部位都有分布,其中以茎尖和根部浓度较高,表现为快速、非极性的运输特性。茎部引入5d之内,植株根内6-BA呈持续升高趋势,茎尖内6-BA虽然有波动,但是最后仍达到最大;而叶片涂抹则在涂抹后5h时,全株各部位6-BA浓度达到最大,5d后,除引入叶片外,其它部位几乎测不到6-BA。盛花后30d,果穗浸蘸和穗轴引入6-BA,2h后,果皮、果肉、种子中6-BA浓度达到高峰,以后逐渐下降,果穗浸蘸30d后,果实各部位均已测不到6-BA,穗轴引入30d后,果皮和果肉内仍能测到6-BA,但种子内已测不到。 相似文献
30.
AIM:To establish the monoclonal antibody against human B lymphocyte stimulator (hBLyS) by DNA immunization and analyse its characterization. METHODS:The 858 bp DNA fragment of hBLyS was cloned into pcDNA3 plasmids. The cloned insert was identified by both sequence analysis and double digestion of the recombinant plasmid with restriction enzymesXho Iand EcoR I. After the splenocytes from BALB/c mice immunized with the recombinant plasmid of pcDNA3/hBLyS were fused with myeloma cells SP2/0,the hybridoma which can produce monoclonal antibodies against hBLyS were obtained. The specificity of anti-BLyS monoclonal antibody from hybridoma was verified by ELISA, Western blot and flow cytometry. RESULTS:The recombinant mammalian cell expression vector of pcDNA3/hBLyS was constructed,the sequence of the insert gene was identified to be the sequence encoding hBLy S antigen. The culture supernatants of hybridoma 9c10 were tested to be the monoclonal antibody with specificity against hBLyS on human peripheral blood CD3+T cell activated by hIFN-γ by ELISA,Western blot and flow cytometry.CONCLUSION:The monoclonal antibodies against hBLyS with high activity and specificity have been established successfully, and will be an useful tool in the studies of relationship between hBLyS and human autoimmunity diseases. 相似文献