H9N2亚型禽流感病毒传播途径特性的比较及其表面基因序列分析

选择中国大陆最早分离的H9N2亚型禽流感病毒(avian influenza virus,AIV)A/Chicken/Guangdong/SS/94(H9N2)(缩写为SS株)和1998年大流行时期分离的H9N2亚型AIVA/Chicken/Shanghai/F/98(H9N2)(缩写为F株)为研究对象,对其在SPF鸡体内的复制能力和传播途径特性比较后发现,F株在4周龄SPF鸡气管中的复制能力高于SS株,F株可以经气溶胶传播途径传播,SS株不能经气溶胶传播途径传播;利用反转录-聚合酶链反应(RT-PCR)方法获取F株和SS株的HA和NA基因的cDNA,序列分析得知,F株和SS株的HA和NA基因的同源性分别是96.6%和98.1%;HA基因的裂解位点氨基酸序列都是PARSSR↓GL,但有5个氨基酸的差异,即166位N(F)→D(SS)、198位A(F)→V(SS)、217位V(F)→I(SS)、335位G(F)→R(SS)、504位L(F)→S(SS);2株病毒的NA基因在63~65位都存在氨基酸缺失,但在NA基因红细胞吸附位点的氨基酸序列不同,分别是IKKDSRSG(F)和IKEDLRSG(SS)。F株和SS株的传播特性差异是否与其表面基因序列有关,有待进一步研究。     

Partial characterization of phytoplasmas associated with lettuce and wild lettuce phyllodies in Iran

Phytoplasmas associated with lettuce phyllody (LP) and wild lettuce phyllody (WLP) in southern Iran were partially characterized by molecular analyses and host-range studies. Agents of both diseases were transmitted by Neoaliturus fenestratus , a leafhopper colonizing lettuce and wild lettuce, to lettuce, wild lettuce, sowthistle and periwinkle, but not to safflower, sunflower, calendula and sesame. Both phytoplasmas induced bud proliferation, virescence, phyllody and witches' broom in infected plants. Total DNA extracted from infected lettuce and wild lettuce or from vector tissues was subjected to PCR using phytoplasma-specific primer pair P1/P7 or nested PCR using P1/P7 followed by R16F2n/R16R2. PCR product of nested PCR (1·2 kbp) was subjected to restriction fragment length polymorphism (RFLP). RFLP analysis of nested PCR product identified the LP, WLP and N. fenestratus -associated phytoplasmas as members of the pigeon pea witches' broom group, 16SrIX. Phylogenetic analysis of the 16S rRNA gene sequence also clustered LP and WLP phytoplasmas with other known members of the 16SrIX group. While no significant differences could be detected between LP and WLP phytoplasmas, both isolates differed from Lebanese wild lettuce phyllody in molecular properties.     

Detection of lethal yellowing phytoplasma in embryos from coconut palms infected with Cape St Paul wilt disease in Ghana

This study investigated the potential of seed transmission of Cape St. Paul wilt disease (CSPWD) in coconuts. PCR amplification was used to assess the distribution of phytoplasmas in parts of West African Tall (WAT) palms infected with CSPWD. Employing phytoplasma universal primer pair P1/P7 in standard PCR, or followed with a nested PCR using CSPWD–specific primer pair G813f/AwkaSR, phytoplasma infection was detected in the trunks, peduncles, spikelets, male and female flowers of four infected WAT coconut palms. Through nested PCR, phytoplasma was also detected in four of 19 embryo DNA samples extracted individually from fruits harvested from three of the four infected palms and was confirmed as CSPWD by cloning and sequencing. Subsequently, CSPWD phytoplasma was again detected in five of 33 embryos from nine infected palms, and in one of eight fruits from two symptomless palms. Fruits from infected palms recorded higher percentage germinations in two field nurseries (average of 71·0%) compared to fruits from healthy palms (average of 57·6%), and matured fruits that had dropped from infected palms showed the same levels of germination as those harvested directly from the palms. This indicates that infected fruits retain the ability to germinate whether harvested or dropped. No phytoplasmas were detected in any of the resulting seedlings and plantlets obtained through embryo in-vitro culture. Therefore, although phytoplasma DNA can be detected in embryos, there is as yet no evidence that the pathogen is seed transmitted through to the seedling to cause disease in progeny palms.     

影响麦二叉蚜传播玉米矮花叶病毒效率的因素分析

玉米矮花叶病毒（ＭＤＭＶ）在田间主要依靠介质蚜虫非持久性方式传播,通过麦二叉蚜（Ｓｃｈｉｚａｐｈｉｓｇｒａｍｉｎｕｍ）对ＭＤＭＶ传毒效率影响因素的分析,发现接毒蚜获毒前禁食与否及禁食时间的长短与传毒效率呈高度正相关,从蚜量与传毒效率的关系来看,多头蚜虫的传毒效率均比单头蚜高,随着ＭＤＭＶ浓度和传毒蚜量的增加,传毒效率也随之提高,单蚜获毒１０ｍｉｎ后不禁食其传毒效率最高,随着禁食时间的延长,其传毒效     

校园视频直播系统的设计与实现

很多学校经常举办大型的会议、论坛、名人讲座和文艺汇演,由于参加人数众多,经常会出现会议场地不够的情况.因此,建立规模适度、经济可行且先进可靠的校园视频直播系统是各学校都在寻求的解决方案.本文从实际出发来探讨校园视频直播系统的设计与实现.     

Shafer气液联动执行机构异常关断事故树分析

输气管道干线截断球阀的气液联动执行机构经常出现误关断的情况,严重影响了管道的安全运行和企业效益.利用事故树定量分析的方法对异常关断事故进行了系统分析.阐述了气液联动执行机构自动关断的原理,利用事故树法详细分析了造成气液联动执行机构异常关断的各种因素,并对各个因素的影响程度进行了定量计算.对可能导致异常关断的29个基本事件进行定量分析后可知：人为失误、ESD误触发、电磁阀掉电、引压管冰堵等因素是造成气液联动执行机构误关断的重要原因.事故树分析结果与管道实际运行情况一致,表明事故树分析方法在实际生产应用中是合理有效的.     

YL2208CW型农用运输车主传动比的模糊优化

作者在建立Ｙ１２２０８ＣＷ型农用运输车动力性与燃油经济性模拟计算方法的基础上，应用模糊数学方法优化农用运输车的主传动器速比，获得了最佳燃油经济性。     

烘炉温度无线检测系统设计

提出了一种检测烘炉温度的无线数字检测系统.该系统将无线收发模块nRF905与单片机系统相结合,实现无线温度检测,其也适用于其他领域设备的温度无线测量.     

对液压传动车辆油液的正确选择及使用

对液压油的分类及物理、化学性能及污染物类型做了简单介绍,主要对液压油的正确选择和使用提出了要求。     

8×4视频切换器控制系统

用ＭＣＳ－５１单片机控制８×４视频切换器，其中４路为视频输出，８路为视频输入，对８路云台和８路镜头进行控制，主控制器可以是一台计算机，专用控制盒或其它设备，本对其硬件实现和软件中使用的命令介绍一种应用方法。