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11.
Blending Bacillus thuringiensis (Bt) corn and non-Bt corn seeds in the same bag is a convenient and easier compliance solution to satisfy refuge regulations. However, there has been considerable debate and concern about larval movement of the target insects in a seed blend because of its potential effect on the development of resistance. Reported here are studies to determine the larval movement of European corn borers, Ostrinia nubilalis (Hübner), in a seed blend compared to a structured refuge and their effects on survival, feeding injury and larval fitness. Also evaluated is the relative performance of a purple-seeded corn hybrid as a surrogate host plant for tracking gene flow. Seed blend and structured refuge arrangements of Agrisure GT/CB/LL Bt corn expressing Cry1Ab and isoline plants were evaluated at two locations in 2013 and 2014 by manually infesting refuge plants with first and second generation corn borer larvae. The majority of leaf and stalk injury was recorded on the refuge plant, which amounted to 66–83% of the total tunnel length. In both refuge arrangements, plant injury and the number of larvae recovered significantly decreased on neighboring plants with increasing distance away from the refuge plant in the same row. The relative decline in injury was much more pronounced in the seed blend, with neighbor Bt plants experiencing very minor tunneling or no injury at all due to the high dose trait. Eighty-nine percent fewer live larvae were also found on neighbor Bt plants in the seed blend compared to those found in the structured refuge plots. Larvae that moved to Bt plants as well as older instars that were manually placed on Bt plants failed to complete development. Comparison of the number of tunnels and larvae recovered per refuge plant indicated that the seed blend may not produce as many susceptible individuals as those produced in a structured refuge arrangement. Although the purple-seeded hybrid showed lower levels of ear and stalk injury, it could serve as an appropriate surrogate refuge plant to track gene flow. 相似文献
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整秆式甘蔗收获机内物流排杂高速摄影分析 总被引:1,自引:0,他引:1
为了研究整秆式甘蔗收获机内物流排杂运动机理,研制了甘蔗收获机内物流排杂试验台,并利用高速摄像机拍摄了甘蔗与蔗叶在物流通道内的运动全过程,并对图像进行分析。结果表明,甘蔗在物流运动过程中发生弹跳、扭转和弯曲变形,在剥叶运动过程中发生断尾,并且通过排杂装置将蔗叶与甘蔗分离后排出。通过建立甘蔗收获机内各部件元件与甘蔗、蔗叶的简化力学模型,结合甘蔗和蔗叶的力学特性分析了甘蔗和蔗叶在物流通道内的运动机理。甘蔗在物流运动中主要受到的作用力为各部件对甘蔗的轴向力、垂直于甘蔗运动方向的切向力和摩擦力。 相似文献
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Black-backed jackals (Canis mesomelas) are an abundant mesopredator on farmlands (croplands and rangelands) across South Africa. Given their adaptability in a changing anthropogenic landscape, knowledge of spatial movements can provide important ecological information on the species. We captured, collared and monitored five black-backed jackals in the Midlands of KwaZulu-Natal to determine their home range and habitat use in farmlands. The 95% fixed kernel (95% FK) home range sizes were large, showing a marked seasonal variation in movement. One adult male dispersed 150?km over two seasons (winter and spring) and thereafter settled into a home range that it maintained. Transient home ranges of males and juveniles were larger than resident home ranges. The mean resident home ranges (95% FK) across seasons for adult males, adult female and juvenile males were 11.4 ± 4.3 km2, 5.6 ± 0.36?km2 and 2.15 ± 0.45?km2, respectively. The mean transient home ranges (95% FK) of all the seasons for adult and juvenile males were 1 181.93 ± 575.81 km2 and 104.27 ± 35.64?km2, respectively. Adult jackal preferred croplands in spring, summer and autumn but avoided croplands in winter. Variable habitat use and large home ranges in our study confirmed the species’ ability to adapt to agricultural areas. 相似文献
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为实现对玉米黄花叶病毒(maize yellow mosaic virus,MaYMV)的血清学检测,丰富该病毒的检测方法,将编码MaYMV运动蛋白(movement protein,MP)的基因连接到原核表达载体pDBHis-MBP上,将构建成功的原核表达质粒转化到大肠杆菌Escherichia coli中诱导表达融合蛋白,将纯化后的融合蛋白对新西兰大白兔Oryctolagus cuniculus进行免疫并制备MaYMV MP多克隆抗血清,并采用Western blot对抗血清的效价、灵敏度和特异性进行检测。结果显示,利用成功构建的原核表达载体经诱导表达获得分子量大小约为62 kD的融合蛋白,纯化后对新西兰大白兔进行免疫获得MaYMV MP抗血清,该抗血清的效价为1∶128 000,灵敏度为1∶32,且该抗血清能够特异性地检测到本氏烟Nicotiana benthamiana中瞬时表达的MaYMV MP,而不与马铃薯卷叶病毒属Polerovirus及黄症病毒属Luteovirus的其他病毒发生血清学交叉反应,证明该抗血清具有良好的特异性。表明本研究制备的MaYMV MP抗血清能特异性... 相似文献
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Lindsay M. Clontz Anni Yang Sarah M. Chinn Kim M. Pepin Kurt C. VerCauteren George Wittemyer Ryan S. Miller James C. Beasley 《Pest management science》2023,79(10):3819-3829
Background
Data on the movement behavior of translocated wild pigs is needed to develop appropriate response strategies for containing and eliminating new source populations following translocation events. We conducted experimental trials to compare the home range establishment and space-use metrics, including the number of days and distance traveled before becoming range residents, for wild pigs translocated with their social group and individually.Results
We found wild pigs translocated with their social group made less extensive movements away from the release location and established a stable home range ~5 days faster than those translocated individually. We also examined how habitat quality impacted the home range sizes of translocated wild pigs and found wild pigs maintained larger ranges in areas with higher proportion of low-quality habitat.Conclusion
Collectively, our findings suggest translocations of invasive wild pigs have a greater probability of establishing a viable population near the release site when habitat quality is high and when released with members of their social unit compared to individuals moved independent of their social group or to low-quality habitat. However, all wild pigs translocated in our study made extensive movements from their release location, highlighting the potential for single translocation events of either individuals or groups to have far-reaching consequences within a much broader landscape beyond the location where they are released. These results highlight the challenges associated with containing populations in areas where illegal introduction of wild pigs occurs, and the need for rapid response once releases are identified. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献18.
大型灌区节水改造后地下水动态的 总被引:1,自引:0,他引:1
以黄河河套灌区的节水灌溉工程改造为目标,通过一个试验区(沙壕渠灌域)的实际分析,在地下水动力学数值模拟基础上,用Galerkin法对节水工程实施后的地下水动态进行预测,通过更大范围(解放闸灌域)用人工神经网络BP模型的快速算法进行了检验比较认为:成果基本可靠,有一定代表性,可供河套灌区水环境评估与宏观水管理预测和决策参考。 相似文献
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