基于模糊分析的农用运输车双万向传动轴可靠性优化设计

将模糊可靠性设计应用于农用运输车传动轴的优化设计,提出了农用车双万向传动轴的模糊可靠性优化设计方法,建立了在满足给定模糊可靠性要求设计条件下优化设计数学模型,并进行了实例计算。     

冷诱导下毛百合甘油-3-磷酸酰基转移酶活性变化及其基因保守区的克隆

探讨了野生毛百合甘油-3-磷酸酰基转移酶活性在冷胁迫下的变化,用简并引物扩增了甘油-3-磷酸酰基转移酶基因保守区序列的结果表明,该保守区段长744 bp,推测编码247个氨基酸.在GPAT氨基酸序列中存在1个高度保守的区域(WIAPSGGRDRP),在NCBI上经过BLASTp比对分析,发现这段保守区序列为LPLAT基...     

Influence of the D genome in conferring resistance to fusarium head blight in spring wheat

Reciprocal crosses were made between resistant hexaploid spring wheat cultivars/lines Sumai 3, Ning8331, and 93FHB21, and susceptible tetraploids Stewart 63 and DT486 to generate 35 chromosome pentaploids. Four heads from each of five F₁ pentaploid plants from each cross were screened with Fusarium graminearum for fusarium head blight (FHB) reaction. No pentaploid was as resistant to FHB as the resistant parents. Pentaploids derived from several crosses were more resistant than the susceptible parents, a few were more susceptible, and all plants from crosses with 93FHB21 failed to survive. Most viable seeds were obtained from the cross Sumai 3 × DT486. From this cross four of the five F₁ pentaploid parents were fertile and 354F₂ seeds derived from these four pentaploids were sown and evaluated for their FHB reaction. The majority of F₂ plants from pentaploids 1 and 3had the visual appearance and level of resistance of Sumai 3, whereas progeny from pentaploids 4 and 5 were more varied morphologically and generally more susceptible. Forty-three of the screened F₂ plants were tested for the presence of specific D chromosomes by wheat microsatellite analysis. There was no relationship between presence/absence of D chromosomes and FHB reaction. Twenty-four lines had all D chromosomes present of which 10 were intermediate-susceptible and 14 were resistant to FHB. Three lines, one resistant and two intermediate, had no D chromosomes. The remainder had between 1 and 6 of the D chromosomes present and ranged from resistant to susceptible in FHB reaction. It appears that FHB resistance is not conferred by the D genome of Sumai 3. This revised version was published online in July 2006 with corrections to the Cover Date.     

土壤微生物DNA木聚糖酶基因多样性的研究

采用国产硅胶Gk254（60型）代替进口Glass bead的改良方法抽提土壤微生物DNA，然后设计了一对扩增木聚糖酶基因片段的新简并引物，对抽提的土壤微生物DNA进行PCR扩增。扩增片段连接pMD18T载体，转化大肠杆菌，重组片段通过酶切进行RFLP分析后，测序分析得到10个木聚糖酶基因片段。对所得片段翻译的氨基酸序列进行BLAST分析表明有8个片段与来自放线菌的木聚糖酶具有较高的同源性，2个与假单胞菌的木聚糖酶具有较高的同源性。所得10个木聚糖酶片段的氨基酸序列同源性比较显示，第27个氨基酸均为天冬酰胺（N），暗示这些来自土壤微生物DNA的基因片段编码耐碱的木聚糖酶。通过构建系统进化树，发现扩增的木聚糖酶片段之间的相似性均在70％以上。     

Comparison of the Value of Pulsed-field Gel Electrophoresis,Random Amplified Polymorphic DNA and Amplified rDNA Restriction Analysis for Subtyping Taylorella equigenitalis

Eight strains of Taylorella equigenitalis were identified by a polymerase chain reaction using a primer pair specific to the 16S rDNA of T equigenitalis. These eight strains were chosen because they had previously been shown to represent eight distinct genotypes by pulsed-field gel electrophoresis analysis after separate digestion of the genomic DNA with ApaI or NotI. The eight strains could be classified into six or seven types by random amplified polymorphic DNA analysis using different kinds of primers. Amplified rDNA restriction analysis after separate digestion with five restriction enzymes, including AluI and MboI, of the 1,500 bp fragments of rDNA amplified by polymerase chain reaction did not discriminate the genomic variations among the eight strains of T equigenitalis. Thus, pulsed-field gel electrophoresis was shown to discriminate these eight organisms better than random amplified polymorphic DNA analysis, while amplified rDNA restriction analysis was found to be unsuitable for subtyping T equigenitalis.     

普陀樟SRAP-PCR反应体系的建立

以舟山群岛的普陀樟（Cinnamomum japonicum var.chenii）新鲜叶片为实验材料,采用L16（45）正交试验设计,对影响普陀樟SRAP-PCR反应的Mg2+浓度、dNTPS、Taq DNA聚合酶、引物和模板DNA用量5因素组合进行了筛选。结果表明：适于普陀樟的SRAP-PCR反应体系（20μL）为2.5 mmol/L Mg2+、0.2 mmol/L dNTPs、1.5 U Taq DNA聚合酶、0.4μmol/L引物、10 ng模板DNA、2μL 10×PCR buffer,该体系位点清晰,扩增稳定;利用该反应体系从100对SRAP引物组合中筛选出多态性好的引物24对。     

创新植保推广模式促进植保技术的普及

为适应现代植保要求,加快植保新技术的普及和应用,高阳县植保植检站通过建立植保专防队、农民培训、实施农业项目、采用现代信息技术等多种植保推广新模式的综合应用,提高了植保新技术的普及率和到位率,创新了植保推广模式,促进了植保技术的推广。     

基于GIS和USLE的九龙江流域土壤侵蚀量预测研究

探讨了GIS和USLE相结合预测南方中等尺度流域土壤侵蚀量、标识流域土壤侵蚀严重区域。运用GIS建立九龙江流域基础地理数据库，利用ARC／INFO的栅格数据空间分析功能，根据USLE土壤侵蚀预测模型对数据库进行图形运算，预测了九龙江流域的土壤侵蚀量。结果表明，流域的年均侵蚀模数为2730．3t／km^2，侵蚀强度属中度。占流域面积85．72％的区域土壤侵蚀强度在中度以下。这一区域对流域土壤侵蚀量的贡献率为58．26％，而流域41．74％的侵蚀泥沙来自于占流域面积14．28％的强度以上侵蚀区域。在流域侵蚀强度的空间分布上，8个子流域属中度侵蚀区，其中船场溪、花山溪和雁石溪三个子流域侵蚀强度较大；6个子流域属轻度侵蚀区，其中漳州平原的龙海和浦南两子流域侵蚀强度最弱。     

使用两种坐标系计算导航距离的研究

对大型农用拖拉机进行导航是目前精准农业的发展方向。对拖拉机导航所涉及的UTM坐标系和空间直角坐标系的投影转换进行了分析,并给出了在这两种坐标系中计算点到空间直线距离的算法。通过编程比较和实际的测量表明,在求解导航距离时空间直角坐标系比UTM坐标系误差大很多,不适合高精度的农机导航中。