猪链球菌蛋白表面展示系统的构建及展示蛋白的初步观察

为构建猪链球菌(Streptococcus suis)蛋白表面展示系统,本研究通过序列分析,确定猪链球菌的LPxTG蛋白及其信号肽(SP)和胞壁锚定基序(CWA),通过PCR扩增Peno-SP、GFP、CWA的DNA片段并融合,构建强启动子Peno控制表达编码SP-GFP-CWA融合蛋白的DNA片段,将该重组DNA片段连接pSET2载体,获得蛋白表面展示质粒,转化猪链球菌,构建得到以GFP为报告蛋白的猪链球菌蛋白表面展示系统。结果显示,利用猪链球菌的10个LPxTG蛋白及其SP和CWA序列,构建了10个含有Peno-SP-GFP-CWA融合片段的重组pSET2表面蛋白展示质粒pSsPSD1至pSsPSD10,分别转化猪链球菌05ZYH33,PCR鉴定显示其中7个转化猪链球菌。采用western blot初步检测其展示蛋白,结果显示,7个转化阳性菌株均能有效表达GFP蛋白,以成熟GFP条带为指标,均表现出了一定的外源GFP表面展示水平,分别命名为SsPSD1、SsPSD2、SsPSD4、SsPSD7-SsPSD10,其中SsPSD1、SsPSD4、SsPSD8和SsPSD9表面展示水平相对较好,在猪链球菌表面展示外源蛋白方面具有很好的潜力。本研究首次尝试建立猪链球菌蛋白表面展示系统,为猪链球菌表面递呈外源蛋白或抗原提供了新的策略。     

Thermal and rheological properties of brown flour from Indica rice

The thermal, paste and rheological properties of brown flours from four Indica rice subspecies with different amylose content were examined using Differential scanning calorimetry (DSC), Brabender Viscometer and rheometer. Peak, final and setback viscosities (p < 0.05) increased with increasing amylose content from Brabender micro Visco-Amylo-Graph (MVA), but the phase transition temperatures of brown rice flour from DSC (p < 0.05) decreased with increasing amylose content. Rheological properties were determined by steady shear, small amplitude oscillatory shear (SAOS) and thixotropic experiments. SAOS results showed a gel-like viscoelastic behavior with G′ higher than G″. Steady-shear results showed that the brown rice flour exhibited a non-Newtonian shear-thinning behavior and the flow curves can be well described by the Herschel-Bulkley model. The upward-downward rheograms showed that brown rice flour gel, except IR-1, had a hysteresis loop, indicating a strong thixotropic behavior.     

水泵机组状态监测与故障诊断系统研究

从20世纪60年代到21世纪，我国的泵站工程为输水、供水以及防洪减灾提供了有力保障。针对水泵机组的状态监测与故障诊断进行研究，建立水泵机组状态监测与诊断的软件及各分析模块与数据库，进行机组性能的预测研究，为水泵机组的状态维修提供支持。     

分块子空间追踪算法

压缩传感理论是一种充分利用信号稀疏性或者可压缩性的全新信号采样理论。该理论表明，通过采集少量的信号测量值就能够实现可稀疏信号的精确重构。本文在研究现有经典重构算法的基础上，提出结合图像分块思想和回溯思想的分块子空间追踪算法（Block Subspace Pursuit, B_SP）用于压缩传感信号的重构。该算法以块结构获取图像，利用回溯过程实现支撑集的自适应筛选，最终实现图像信号的精确重构。实验结果表明，在相同测试条件下，该算法的重构效果无论从主观视觉上还是客观数据上都有不同程度的提高。     

二氧化碳基塑料降解性能的研究

[目的]研究二氧化碳基塑料的降解性能.[方法]以PPC为原料,通过生物降解、热氧化降解和光降解研究了PPC的降解性能.利用乌式粘度计测定了降解过程中PPC塑料的分子量变化;利用傅里叶变换红外光谱仪表征了降解过程中PPC的红外光谱特性.[结果]PPC薄膜在27℃恒温培养箱中生物降解15 d后,降解效果明显.180℃热氧化降解36 h,PPC塑料的分子量减少了63％.光氧化降解5d后,PPC塑料的分子量减少了约85％.[结论]与生物降解相比,PPC塑料的热氧化和光降解程度更为明显.     

机动车废气分析仪信号处理系统的智能化设计

提出了机动车废气分析仪的智能化组成及其实现信号处理的全过程。详细阐述了信号处理的软硬件的实现。实验结果表明：本系统克服了传统不分光红外线分析仪固有的缺陷。进一步保证了测量数据的正确性和准确性。     

Portable ECG Monitor Front- ended Design with C8051 F020

The electrocardiogram (ECG) signal is a recording of the electrical activity on the body surface generated by the heart frequency of which is weak and low. Many researchers devote themselves to get more perfect ECG in many ways. This article introduces the characterize of an ECG signal , then discusses the requirements for the sampling circuit of an ECG. Since there are some shortcoming in common design means, the authors give an effective circuit. The system is composed of a low-cost , low-power and high-performance MCU C8051F020 , amplifier AD620 , right-leg drive circuit ,communication circuit and power management circuit. Then software design is given which is used to sampling and filtering ECG signal. Levkov filter arithmetic and smooth filter arithmetics are used in this design. In the end , the authors advance a distributing and networking ECG monitor system.