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本文采用不同扦插基质、不同植物生长调节剂及同一植物生长调节剂-ABT的不同浸泡时间,观察宁杞7号硬枝扦插成活率的影响,结果表明以上层东北泥炭+下层净河沙=2∶1的扦插基质生根效果最好,成活率达86.7%。在不同植物生长调节剂与浸泡时间对插穗生根效果的影响中,以ABT生根粉生根效果较好,浸泡时间宜采用4-5h,成活率分别达88.3%和81.7%。 相似文献
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The plant infection method is commonly used to estimate the Most Probable Number (MPN) of soil rhizobia. Here, a qPCR method was set-up and validated with newly developed ANU (strain specific) and RHIZ (more general) primers to quantify the specific Rhizobium leguminosarum bv. trifolii ANU843 strain or general R. leguminosarum strains. Detection limits of qPCR protocols in soil were 1.2 × 104 (ANU) and 4.2 × 103 (RHIZ) cells per g soil. The qPCR assay appears robust and accurate in freshly inoculated soils but overestimated MPN for indigenous soil rhizobia. An incubation experiment showed that qPCR detected added DNA or non viable cells in soils up to 5 months after addition and incubation at 20 °C in moist conditions. 相似文献
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[目的]探索生长刺激素对油茶芽苗砧嫁接的影响,以培育粗壮的胚芽来提高嫁接成活率。[方法]用生长刺激素911生根剂、GA3和ABT生根粉处理油茶种子,比较不同的处理对种子发芽率、发芽速度、胚芽粗度、嫁接成活率的影响。[结果]3种生长刺激素处理对胚芽的生长势产生了明显的影响,无论是发芽速度、胚芽生长量,还是嫁接成活率,采用激素处理的都比对照好得多。911生根剂、GA3、ABT生根粉处理的油茶平均发芽速分别比对照快5、7和9d,胚芽粗度分别比对照提高45%、40%和50%,嫁接成活率分别比对照高8%、12%和9%。[结论]使用3种激素对油茶催芽,均能促进生根和侧根、须根发育,提高其嫁接成活率,其中GA3处理效果最好。 相似文献
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Maizon DO Oltenacu PA Gröhn YT Strawderman RL Emanuelson U 《Preventive veterinary medicine》2004,66(1-4):113-126
The objectives of this study were to evaluate the effect of diseases on days open (DO), days to first breeding (DFB) and days from first breeding to conception (DFBC) using survival analysis models, and to assess the significance of the sire component and its possible confounding effect. The data consisted of a random sample of 20% of all herds enrolled in Swedish recording system and using 100% artificial insemination with at least 15 Swedish Red and White cows calving in 1991. The follow-up period was from 45 to 145 d after calving. After editing, the data sets had 23,927, 28,197, and 22,089 cows for days open, days to first breeding, and days from first breeding to conception, respectively. The Cox models included parity, calving season, cow milk production and age at first calving as fixed effects, and herd and sire as random effects. Ten disease groups were considered as possible risk factors for the reproductive traits. Disease groups were treated differently if they occurred before or after 45 d postpartum. Diseases occurring in the first 45 d after calving were treated as time-independent covariates and diseases occurring after day 45 were treated as time-dependent covariates for days open and days to first breeding. The percentages of censored cows were 35% for days open, 19% for days to first breeding, and 33% for days from first breeding to conception. Days open increased in cows with dystocia, stillbirth, retained placenta, metritis, or other diseases occurring in the first 45 d after calving, and in cows with metritis, mastitis, or other diseases occurring after 45 d. Days to first breeding increased in cows with stillbirth, retained placenta, milk fever, mastitis, foot and leg problems, or other diseases occurring before day 45, and in cows with metritis, mastitis, foot and leg problems, or other diseases occurring after 45 d. Days to first breeding decreased in cows treated for ovulatory dysfunctions either before or after 45 d. Days from first breeding to conception increased in cows with dystocia, stillbirth, retained placenta, metritis, or ovulatory dysfunctions occurring before first breeding, and in cows with mastitis occurring after first breeding. Although the additive genetic components were significant for all traits considered, the sires did not act as confounders because only a small amount of variability for the traits considered in this study was explained by the sires, with estimated heritabilities of 2% on the logarithmic scale and from 3 to 4% on the real scale. 相似文献
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怀山药种质资源的玻璃化法超低温保存 总被引:11,自引:0,他引:11