种植模式对苜蓿固氮和固氮产物转移的影响

田间小区试验研究了不同种植模式下苜蓿的共生固氮贡献,并利用~(15)N同位素示踪技术评估了苜蓿的％Ndfa和Ndfa,以及与之混作生长的牛尾草植株中来自苜蓿固氮产物的转移量。研究表明,豆科与禾本科牧草混作对发挥草地的优势有一定影响,混作条播在干草产量、全氮产量、％Ndfa和Ndfa等方面均优于间作与混作撒播模式,且高于单作苜蓿与牛尾草的平均值。用~(15)N同位素稀释法与~(15)N天然丰度法评估苜蓿的％Ndfa与Ndfa值时,无明显差异(P<0.05),前者还能准确测出混种牛尾革植株中的固氮产物转移量,后者则大大低估,甚至不能测出固氮产物转移。     

基于稳定同位素技术的天津大神堂海域人工鱼礁区食物网结构研究

本研究对2016年6月在天津大神堂3个礁区(2010年建成的鱼礁区;2012年建成的鱼礁区;2014年建成的鱼礁区)和对照区域采集的生物消费者及其食物源样品的碳、氮稳定同位素组成进行了分析,通过IsoSource模型计算不同区域生物的食物网基础,并利用氮稳定同位素数据计算消费者的营养级。结果显示,根据δ13C值可以将其食物源分为浮游植物、悬浮颗粒有机物(POM)和沉积相颗粒有机物(SOM)三类;浮游植物对消费者的碳源贡献率(67.2%~81.5%)最大,是大神堂海域的生物食物网的基础。不同区域同一食物源的δ13C和δ15N值没有显著性差异;礁区内滤食性贝类毛蚶(Arca subcrenata)、菲律宾蛤仔(Ruditapes philippinarum)和长牡蛎(Crassostrea gigas)的δ13C值显著高于对照区,作为潜在碳源的浮游植物对其的贡献率显著增加。消费者的δ15N值则介于7.70‰~14.34‰之间,消费者的营养级介于2.0~3.95级之间。游泳生物食性生物的δ15N和营养级在礁区内有所提高,礁区建成的年份越长,其营养级与对照区域的差别越显著。稳定同位素研究表明,人工鱼礁建设可能导致鱼礁区内滤食性生物的食物来源组成改变,并提高游泳生物食性生物的营养级。     

西伯利亚落叶松树轮稳定碳同位素对气候的响应

利用采自阿尔泰山南坡东段正格采点的西伯利亚落叶松树轮样本,建立树轮宽度年表,对比宽度年表,按照树轮稳定碳同位素研究步骤,提取树轮稳定碳同位素序列（δ¹³C）和去趋势序列（DS）。分析表明：阿尔泰山南坡东部近160 a西伯利亚落叶松树轮δ¹³C变化于-22.8‰～-25.4‰,平均值为-24.0‰,变差系数-0.017。相关分析表明：正格采点树轮宽度序列对各气候要素相关不显著;树轮去趋势序列（DS）对前期气候因子的响应也不敏感;在生长季内（5~9月）,树轮去趋势序列（DS）与富蕴气象站平均气温、平均最高气温和平均相对湿度有较好的相关性,树木生长季内的平均气温、平均最高气温与树轮去趋势序列（DS）显著正相关,6~7月平均最高气温与去趋势序列（DS）相关达到0.611（P<0.000 001);与生长季内平均相对湿度呈负相关关系,其中6~7月相对湿度与去趋势序列（DS）相关最高(r=-0.493, P<0.001),与7月降水量呈负相关关系(r=-0.459, P<0.01)。生长季内的平均最高气温和相对湿度对阿尔泰山西伯利亚落叶松树轮δ¹³C的响应最为敏感,阿尔泰山西伯利亚落叶松树木碳同位素分馏的主要控制因子为6~7月的平均最高气温,降水对树轮稳定碳同位素影响不大,相对湿度对树轮稳定碳同位素没有直接影响。     

甘肃梨园河流域地下水来源及其水化学特征

通过分析水化学与氢氧稳定同位素的关系,研究梨园河流域地下水的补给机理和水化学演化规律。地下水总溶解固体（TDS）自西南向东北递增,最大值为1 258 mg/L。沿流程方向,深层地下水的水化学类型由Ca-HCO₃→Ca-Mg-HCO₃、Mg-SO₄→Na-SO₄型。浅层地下水的水化学类型由Ca-HCO₃→Mg-HCO₃-SO₄→Mg-SO₄型。地表水的水化学类型无分异,主要为Ca-HCO₃型。深层和浅层地下水沿途均发生了水岩融滤作用。Na⁺含量沿程增大,一是地下水中Ca²⁺与岩石中Na⁺发生阳离子交换作用,二是硅酸盐矿物的风化作用产生Na+。Phreeqc软件模拟显示,深层地下水中方解石先沉淀后溶解;CO₂、石膏、白云石和岩盐溶解。浅层地下水中,方解石由不饱和逐渐变为饱和;石膏、岩盐一直溶解。地下水的δ²H和δ¹⁸O值大部分位于大气降水线上方,反应了山区现代降水或雪冰融水通过出山地表径流补给,交替更新快,且深层地下水向上补给浅层地下水和地表水。地表、地下水相互转化是该区水循环的主要特征。     

Role of nitrite and nitric oxide in the processes of nitrification and denitrification in soil: Results from N tracer experiments

Recent research has proven soil nitrite to be a key element in understanding N-gas production (NO, N₂O, N₂) in soils. NO is widely accepted to be an obligatory intermediate of N₂O formation in the denitrification pathway. However, studies with native soils could not confirm NO as a N₂O precursor, and field experiments mainly revealed ammonium nitrification as the source of NO. The hypothesis was constructed, that the limited diffusion of NO in soil is the reason for this contradiction. To test this diffusion limitation hypothesis and to verify nitrite and NO as free intermediates in native soils we conducted through-flow (He/O₂ atmosphere) ¹⁵N tracer experiments using black earth soil in an experimental set up free of diffusion limitation. All of the three relevant inorganic N soil pools (ammonium, nitrite, nitrate) were ¹⁵N labelled in separate incubation experiments lasting 81 h based on the kinetic isotope method. During the experiments the partial pressure of O₂ was decreased in four steps from 20% to about 0%. The net NO emission increased up to 3.7 μg N kg⁻¹ h⁻¹ with decreasing O₂ partial pressure. Due to the special experimental set up with little to no obstructions of gas diffusion, only very low N₂O emission could be observed. As expected the content of the substrates ammonium, nitrate and nitrite remained almost constant over the incubation time. The ¹⁵N abundance of nitrite revealed high turnover rates. The contribution of nitrification of ammonium to the total nitrite production was approx. 88% under strong aerobic soil conditions but quickly decreased to zero with declining O₂ partial pressure. It is remarkable that already under the high partial pressure of 20% O₂ 12 % of nitrite is generated by nitrate denitrification, and under strict anaerobic conditions it increases to 100%. Nitrite is present in two separate endogenous pools at least, each one fed by the nitrification of ammonium or the denitrification of nitrate. The experiments clearly revealed that nitrite is almost 100% the direct precursor of NO formation under anaerobic as well as aerobic conditions. Emitted N₂O only originated to about 100% from NO under strict anaerobic conditions (0-0.2% O₂), providing evidence that NO is a free intermediate of N₂O formation by denitrification. To the best of our knowledge this is the first time that NO has been detected in a native soil as a free intermediate product of N₂O formation at denitrification. These results clearly verify the “diffusion limitation” hypothesis.     

N<Subscript>2</Subscript> fixation by faba bean (<Emphasis Type="Italic">Vicia faba</Emphasis> L.) in a gypsum-amended sodic soil

The response of faba bean to the application of four rates of gypsum (0, 2.5, 5.0, 10.0 t ha⁻¹) to a non-saline, alkaline sodic soil was measured in terms of grain yield, dry matter (DM) production, N accumulation and the proportional dependence of the legume on symbiotic N₂ fixation (P _atm). A yield-independent, time-integrated ¹⁵N-dilution model was used to estimate symbiotic dependence. A significant decrease in the exchangeable sodium percentage and significant increases in exchangeable Ca⁺⁺ and the Ca⁺⁺:Mg⁺⁺ ratio in the 0–10-cm soil layer were measured 30 months after application of 10 t ha⁻¹ gypsum. Despite low and erratic rainfall during crop growth, faba bean DM and N uptake responded positively to gypsum application. The symbiotic dependence of the legume at physiological maturity was little affected by sodicity (P _atm = 0.74 at zero gypsum and 0.81–0.82 at 2.5–10 t ha⁻¹ gypsum). The increase in fixed N due to gypsum application was mainly due to increases in legume DM and total N uptake. At 10 t ha⁻¹ of gypsum, faba bean fixed more than 200 kg N ha⁻¹ in above-ground biomass.     

Organic matter turnover in soil physical fractions following woody plant invasion of grassland: Evidence from natural C and N

Soil physical structure causes differential accessibility of soil organic carbon (SOC) to decomposer organisms and is an important determinant of SOC storage and turnover. Techniques for physical fractionation of soil organic matter in conjunction with isotopic analyses (δ¹³C, δ¹⁵N) of those soil fractions have been used previously to (a) determine where organic C is stored relative to aggregate structure, (b) identify sources of SOC, (c) quantify turnover rates of SOC in specific soil fractions, and (d) evaluate organic matter quality. We used these two complementary approaches to characterize soil C storage and dynamics in the Rio Grande Plains of southern Texas where C₃ trees/shrubs (δ¹³C=−27‰) have largely replaced C₄ grasslands (δ¹³C=−14‰) over the past 100-200 years. Using a chronosequence approach, soils were collected from remnant grasslands (Time 0) and from woody plant stands ranging in age from 10 to 130 years. We separated soil organic matter into specific size/density fractions and determined their C and N concentrations and natural δ¹³C and δ¹⁵N values. Mean residence times (MRTs) of soil fractions were calculated based on changes in their δ¹³C with time after woody encroachment. The shortest MRTs (average=30 years) were associated with all particulate organic matter (POM) fractions not protected within aggregates. Fine POM (53-250 μm) within macro- and microaggregates was relatively more protected from decay, with an average MRT of 60 years. All silt+clay fractions had the longest MRTs (average=360 years) regardless of whether they were found inside or outside of aggregate structure. δ¹⁵N values of soil physical fractions were positively correlated with MRTs of the same fractions, suggesting that higher δ¹⁵N values reflect an increased degree of humification. Increased soil C and N pools in wooded areas were due to both the retention of older C₄-derived organic matter by protection within microaggregates and association with silt+clay, and the accumulation of new C₃-derived organic matter in macroaggregates and POM fractions.     

淹水土壤中久效磷的降解及消失动态

本试验在实验室模拟土壤淹水条件下,研究微生物对久效磷残留及降解的影响。结果表明:在未经灭菌的土壤中,久效磷及其降解物的消失比在灭菌土壤中快,相应的回归方程为C_WT=5.4502e~(-0.0254)t,C_YT=5.8509—0.0423t,残留半衰期分别为27.3和69.2天。久效磷在淹水土壤中降解为甲醇可抽提态和不可抽提态残留物,对甲醇可抽提态残留物经TLC和红外光谱分析表明,其降解物为磷酸三酯、N-去甲基久效磷、O,O-二甲基乙烯基磷酸酯,0-去甲基久效磷,以及O,C-去甲基久效磷,但主要为磷酸三酯,其含量由母液中的5％增加到28—83％,这种降解过程,在未灭菌土壤中比在灭菌土壤中快。     

Effect of successive cuttings on uptake and partitioning of 15N among plant parts of Leucaena leucocephala

Summary We studied the effect of three successive cuttings on N uptake and fixation and N distribution in Leucaena leucocephala. Two isolines, uninoculated or inoculated with three different Rhizobium strains, were grown for 36 weeks and cut every 12 weeks. The soil was labelled with 50 ppm KNO₃ enriched with 10 atom % ¹⁵N excess soon after the first cutting. Except for the atom % ¹⁵N excess in branches of K28 at the second cutting, both the L. leucocephala isolines showed similar patterns of total N, fixed N₂, and N from fertilizer distribution in different parts of the plant at each cutting. The Rhizobium strain did not influence the partitioning of ¹⁵N among the different plant parts. Significant differences in ¹⁵N enrichment occurred in different parts. Live nodules of both isolines showed the lowest atom % ¹⁵N excess values (0.087), followed by leaves (0.492), branches (0.552), stems (0.591), and roots (0.857). The roots contained about 60% of the total plant N and about 70% of the total N derived from fertilizer over the successive cuttings. The total N₂ fixed in the roots was about 60% of that fixed in the whole plant, while the shoots contained only 20% of the fixed N₂. We conclude that N reserves in roots and nodules constitute another N source that must be taken into account when estimating fixed N₂ or the N balance after pruning or cutting plants. ¹⁵N enrichment declined up to about fivefold in the reference and the N₂-fixing plants over 24 weeks following the ¹⁵N application. The proportion and the amounts of N derived from fertilizer decreased, while the amount derived from N₂ fixation increased with time although its proportion remained constant.     

有机肥与无机肥配施对菜地土壤N2O排放及其来源的影响

该研究采用同位素自然丰度法,通过室内培养试验研究北京地区菜地有机肥和无机肥配施对土壤释放N2O及同位素位嗜值SP（site preference）的影响,以期获得不同肥料及其配比下土壤N2O的来源及变化规律。结果表明：施用无机肥释放的N2O显著高于有机肥,其累积排放量是有机肥的6.63倍,且无机肥施用比例越高,排放量越大;各肥料组合在施用后7天内均以反硝化作用生成N2O为主,贡献最高达到78.89%,SP为6.97‰,之后硝化作用逐渐增强并成为主要途径,最高占比达76.48%,SP为25.24‰;培养期内施用无机肥可以促进反硝化作用,平均占比52.98%,SP为15.52‰,而有机肥会使硝化作用增强,平均占比71.35%,SP为23.55‰。因此,在北京潮褐土地区菜地土壤施用有机肥对N2O有良好的减排效果,可为蔬菜生产中肥料的合理应用提供科学依据。