青海血蜱不同发育阶段蛋白分析

考虑到不同发育阶段蛋白多肽及抗原特性方面的差异,本实验利用SDS-PAGE和Westernblotting对青海血蜱的卵、幼蜱、若蜱、成蜱的蛋白进行了分析。SDS-PAGE结果显示,卵出现了7条主带,幼蜱出现了16条主带,若蜱出现了14条主带,成蜱出现了12条主带。它们的蛋白多肽在数量、含量和分子量上存在差异,共同带只有4条,但相邻发育阶段之间的共同带较多。用感染羊血清和免疫羊血清作免疫印迹时,感染羊血清在分子量5.6万处有明显的反应带,免疫羊血清在分子量9.7万处有明显的反应带。     

大豆抗旱的生理生态基础

大豆抗旱的生理基础和生态基础 ,前人已做过研究。综述前人在大豆抗旱的形态特征、解剖组织特点及影响抗旱的各生态因子方面的研究成果 ,为大豆的高产优质低耗栽培提供理论依据     

Quality evaluation of commercial chile-based salsas

A standardized extrusion cooking process was developed for production of a high protein weaning food based on peanuts, maize and soybeans. Major factors evaluated included the effects of blend formulation, extrusion temperature and feed moisture content on ease of extrusion and product quality characteristics. Results showed bulk density and hardness increased while expansion index decreased with increase in feed moisture content. At a fixed range of feed moisture content, product bulk density and firmness decreased while expansion index increased with increasing extrusion temperature. For ease of extrusion and best product quality in terms of sensory attributes and cooking properties, the following extrusion parameters were established for a blend formulation of 75% maize, 10% peanut and 15% soybean: feed particle size of 300–400 m extruded using a screw speed of 500 rpm, with a feed rate of 4.6 kg/min, feed moisture content of 16–18%, and extrusion temperature of 100 ,°C–105,°C. Pair-wise comparison of the sensory attributes of porridges prepared from milled samples of the weaning foods showed significant differences between extruded products and existing traditional counterparts, with very high scores for all sensory attributes of the extruded products, especially extruded raw (non-roasted) blend samples. In the Home-Use-Test, at least 92% of respondents in two out of the three major ecological zones of Ghana placed overall sensory and functional characteristics of extruded raw blend samples as highly acceptable. About 7% of respondents scored sensory and functional quality attributes as acceptable.     

GmFW1 expression decreased in GmSymRK knockdown transgenic soybean roots

SymRK and GmFWL1 both play important roles in nodulation. However, during symbiotic development, the details of Nod factor signaling association with the regulation of cell division in nodules are unknown. SymRK, the immediately downstream component of these Nod factor receptors, is central to the Nod factor signaling cascade. In this study, specified RNAi plasmid of GmSymRK was constructed and transformed into soybean roots by agrobacterium rhizogenes-mediated hairy root transformation. We found that the nodule number decreased substantially in GmSymRK knockdown soybean transgenic roots. Further to study the relationship between GmFWL1 and Nod factor signaling, we analyzed the GmFWL1 expression levels in the GmSymRK RNAi soybean transgenic roots and found that rhizobia inoculation led to substantially reduced GmFWL1 expression in GmSymRK RNAi soybean transgenic roots. Our studies showed that the regulation of cell division was affected by Nod factor signaling during nodule development in soybean, which provides important information toward understanding the functions of GmSymRK and GmFWL1 in symbiotic signaling and nodule development.     

混施微肥对不同类型大豆产量性状的影响

试验通过拌种结合叶面喷肥的施肥方式,研究了硼、钼、锌、锰、铁微量元素对铁丰30号、铁丰31号和铁豆37号的产量性状的影响.结果表明,该施肥方式下施用不同剂量的微量元素,均增加了不同类型大豆的单株荚数、每荚粒数、单株粒数、分枝数和籽粒产量,但对其百粒重和主茎节数影响较小.     

小麦茬地免耕大豆精密播种机性能试验研究

从大豆种子的株距、播深和施肥位置三个方面测试小麦茬地免耕大豆精密播种机的工作性能,并做了田间试验,结果表明:该机株距合格指数可达68.4%,重播指数为13.4%,漏播指数为18.1,变异系数为26.3,播深合格率为87.7%,达到了<中耕作物精密播种机产品质量分等>标准中关于一等品的要求.同时,施肥位置也满足农艺要求.     

大豆育种进展与前景展望

通过对国内外大豆的生产现状、大豆育种常用方法及优缺点、未来发展方向及前景展望的分析,指出今后我国大豆育种目标是主攻单产,兼顾高蛋白和高油,同时选取具有更广适应性、更高抗病虫性的品种。同时要广泛引入国外优异资源,采用常规鉴定技术和生物技术挖掘资源的遗传潜力,为育种提供优秀的亲本和优良基因;在育种方法上应采用生物技术与常规育种相结合的方法,构建和识别优异的变异个体,变传统育种为现代育种,使大豆品种在培育方面迈上新台阶。     

酶法脱胶工厂化试验

介绍了酶法脱胶的基本原理、工艺流程及在工厂中的应用优点,通过实践获得了最佳生产条件:酶反应温度50～55℃、pH5.0左右,并与化学精炼的经济性进行了对比,同时总结了生产中存在的问题.     

不同结荚习性大豆品种生育特性的研究

通过对不同结荚习性大豆品种各生育阶段的长短、生长发育的速度和植株上、中、下各部位的产量分布、子粒内含品质的测定,明确了不同结荚习性大豆品种各自的生育规律与特点。结果表明,不同结荚习性大豆品种子粒的大小和子粒蛋白质与脂肪总含量与子粒产量在各部位分布的规律基本一致。研究还进一步讨论了不同结荚习性大豆品种的形成机理、子实产量形成的重点时期与部位及其对环境条件的适应特点与分布规律,以及在优质高产栽培中应注重其不同的重点促进期。