不同种源非洲桃花心木苗期抗寒能力和生长节律研究

2008年1月上旬至2009年7月下旬,对非洲引进的3个桃花心木种源的幼树进行每周1次的观测.结果显示:种源间抗寒能力、树高生长和地径生长以及速生期都存在明显差异.种源3-1324与4-1325在苗期表现出一定的抗寒性,种源1-1320苗期对低温敏感.总体上,非洲桃花心木幼树1年抽梢4～7次,1～2月为树高生长休眠期,...     

The whole amino acid profile as indicator of the nutritional condition in cultured marine fish larvae

The effect of variations in the amount or quality of food provided on the amino acid profile of larvae, was tested in two marine fish species, the Senegal sole (Solea senegalensis) and the sea bass (Dicentrarchus labrax). The objective was to assess whether such nutritionally suboptimum dietary patterns, which simulate situations that may occur in hatcheries, result in variations in the concentration of one or more amino acids that could be used as indicators of nutritional condition of larvae. Restriction in the normal food ration by 60% had no clear effect on the amino acid profile of sea bass, while the concentration of some amino acids (Arg, Ala and Phe) showed significant variations in sole. Feeding on artificial diets, which have shown their inadequacy a priori, resulted also in no significant effect on the profile of sea bass, but in a dramatic modification of the amino acid profile in sole. In addition, changes in the amino acid profiles considered as a whole were evaluated using the cluster analysis instead of paired comparisons between amino acid concentrations in each treatment. The analysis clearly separated profiles of larvae fed restrictedly or inadequately from their controls, irrespective of the species. This demonstrated the potential value of evaluating whole changes in amino acid profile as nutritional indicator. Besides, it was demonstrated that sensitivity of larvae to nutritional changes that may be reflected in the amino acid profile is greatly conditioned by the species and developmental stage.     

Growth, stress response and free amino acid levels in Senegalese sole (Solea senegalensis Kaup 1858) chronically exposed to exogenous ammonia

Stressful husbandry conditions are likely to affect growth and amino acid metabolism in fish. In this study, chronic ammonia exposure was used to test the effects of a stressor on growth and amino acid metabolism of Senegalese sole juveniles. The fish were exposed for 52 days to 11.6 mg L⁻¹ [low‐TAN (L‐TAN)] or 23.2 mg L⁻¹ [high‐TAN (H‐TAN)] of total ammonia nitrogen (TAN), or to 0 mg L⁻¹ (Control). Growth in L‐TAN groups was slightly but significantly different from the Control groups [relative growth rate (RGR=0.35±0.13 and 0.52±0.23% day⁻¹ respectively)]. In H‐TAN groups, growth was severely affected (RGR=0.01±0.13% day⁻¹). Stress parameters (plasma cortisol and glucose) showed slight or no significant differences between treatments. Plasma free amino acid (FAA) concentrations were affected after H‐TAN treatment. Increases in glutamine and aspartate concentrations in H‐TAN fish suggest alterations in amino acid metabolism related to nitrogen excretion processes. Some of the changes in FAA concentrations also suggest mobilization to energy supply and synthesis of metabolites related to stress response. Therefore, Senegalese sole seem to adapt to the L‐TAN concentration tested, but the H‐TAN concentration reduced growth and affected amino acid metabolism.     

Thyroid gland development in Senegalese sole (Solea senegalensis Kaup 1858) during early life stages: A histochemical and immunohistochemical approach

A key to success in the culture of marine fish species is the mass production of high quality fry, a process largely dependent on successful first feeding and normal development and growth of fish larvae. In this regard it is important to examine the structural and functional development of the endocrine system (pituitary, thyroid, interrenal glands) during early ontogeny of marine fish. Particularly, the thyroid hormones, thyroxine (T₄) and triiodothyronine (T₃), influence numerous metabolic processes, such as growth, differentiation, metamorphosis, reproduction, respiration, migratory behaviour, central nervous system activity, seasonal adaptation, etc. Therefore the aim of this study was to describe the development of the thyroid gland and the ontogeny appearance of the thyroid hormones in Solea senegalensis larvae by means of histological and immunohistochemical techniques. The first thyroid follicle was present at 4 days-post-hatch (dph) coinciding with first feeding. During metamorphosis (12–20 dph) the follicles increased in both number and size, and by 30 dph presented the same characteristics as that seen in adult fish. Tissue immunostaining of both thyroid hormones decreased during the endogenous larvae development to nearly undetectable levels at the completion of yolk-sac absorption. During larvae exogenous phase, T₃ and T₄ immunostaining was first detected by 6 dph and an increase of specific staining for both hormones was detected between 12 and 20 dph, during metamorphosis phase.     

Genetic structure and genetic relatedness of a hatchery stock of Senegal sole (Solea senegalensis) inferred by microsatellites

Solea senegalensis is a fish species in an early stage of domestication. A commercial hatchery in southern Spain experienced a dramatic failure in spawning and embryo survival, after incorporation into the broodstock of fish reared in their hatchery. To assess the impact of management on the stock quality, a population genetics study was made of adult broodstock present at this hatchery in 2000. The broodstock was composed of a group of fish of wild origin and two more groups from F₁ progeny. A set of eight microsatellite loci was used to compare genetic variability among the three groups and to establish the relationships between pairs of individuals within each group.Individuals from F₁ origin showed a substantial reduction in genetic variability when compared to those from the wild. There was a greater than 50% decrease in the number of alleles per locus corrected by sample size and 16% and 26% reductions in H_e values in the two F₁ groups, respectively. The degree of genetic relationship between individuals, evaluated by the relatedness estimator, rxy, and by graphical approaches, revealed a high proportion, almost 75%, of full-sibs and half-sibs in both F₁ groups, which might account for the reduction in overall variability. The high proportion of siblings also suggests a reproductive pattern in this stock in which few individuals breed successfully. This mating behaviour, along with factors inherent to selection and management systems, might be responsible for the negative effects on the reproductive capacity of the stock.     

Tolerance response to water pH in larvae of two marine fish species, gilthead seabream, Sparus aurata (L.) and Senegal sole, Solea senegalensis (Kaup), during development

Gilthead seabream, Sparus aurata (L.) and Senegal sole, Solea senegalensis (Kaup) are two fish of primary importance in Mediterranean aquaculture. In the present study, the larvae of these species were exposed to different rearing‐water pH during 24 h to examine their tolerance. The 24‐h pHL50 values were calculated with low and high pH values at 7, 20 and 32 days after hatching (DAH) in S. senegalensis larvae, and 12, 20 and 52 DAH in S. aurata larvae. Low 24‐h pHL50 values ranged between 4.88 and 5.76, whereas high 24‐h pHL50 values ranged between 8.94 and 9.57 in S. senegalensis larvae. S. aurata larvae showed values of low 24‐h pHL50 that ranged between 4.82 and 5.55, whereas values of high 24‐h pHL50 ranged between 8.66 and 9.26. Both species showed similar tolerance response at all the tested ages. The high 24‐h pHL50 values found were close to pH values that eventually can be reached in the rearing tanks. The pH should be carefully controlled in rearing water during the first development stages of both species.     

非洲桃花心木大树移植的生长效应研究

大树移植是现代城市园林植物景观建设中常见的栽培措施.对不同移植年龄的非洲桃花心木大树移植后的生长情况、受寒害程度以及寒害后的恢复生长情况进行研究.结果表明,非洲桃花心木的生长在不同移植年龄有显著差异;不同移植年龄的1～2年生平均粗度在14.5～19.5 mm的枝条均受到寒害而枯,受伤枝条平均长度范围为0.79～0.88 m;寒害后,不同株间的萌芽数差异较大,但各个方向的枝条生长无显著差异,不同季节间的枝条生长存在极显著差异,且春夏的粗度、长度极显著大于秋冬的粗度、长度.     

Feeding ragworm (Nereis virens Sars) to common sole (Solea solea L.) alleviates nutritional anaemia and stimulates growth

Common sole fed with commercial pellets develop anaemia and are restricted in their growth performance. The anaemia can be the result of a difference in feed intake, a nutritional deficiency, an inflammatory response to infection or combinations of these aspects. In this study, it was investigated whether feeding ragworm would alleviate the anaemia and stimulate growth. Sole were fed one of three diets: a commercial feed, a commercial feed treated with ragworm extract or chopped ragworm. By comparing groups, three hypotheses were tested: (1) feeding ragworm alleviates the anaemia and positively affectS the sole's metabolic performance reflected in feed intake, feed efficiency and growth; (2) anaemia is alleviated by a higher feed intake when feeding ragworm and (3) anaemia is caused by an inflammatory response to infection. The sole fed with a commercial diet suffered from anaemia. Feeding sole with ragworm alleviated the anaemia as the average haematocrit level nearly doubled in these fish as compared to fish fed pellets. Investigation of the expression of genes in the liver indicated that the anaemia in sole fed pellets is a nutritional anaemia and not an anaemia due to an inflammatory response. Sole fed ragworm showed improved growth which may be a consequence of the higher haematocrit levels in these fish increasing their oxygen carrying capacity. Addition of ragworm extract to the pellets levelled the feed intake between pellets and ragworm, but did not improve the anaemic state of sole and had only a limited effect on growth.     

High stocking density and food restriction have minimum impact on size dispersal of cultured Senegalese sole (Solea senegalensis, Kaup 1858) juveniles. Evidence for individual growth being regulated by population structure

The individual growth being regulated by population structure was studied in cultured populations of Senegalese sole (Solea senegalensis Kaup) juveniles. Soles constituting an initial population were individually tagged and their specific growth rates (SGR) registered after two months of growth. Fish were then graded in order to achieve four new subpopulations based in their previous growths and cultured for other additional 60 days. Three subpopulations consisted of individuals that had previously shown an average SGR (% day⁻¹) of 1.04 ± 0.3, 0.66 ± 0.1 and 0.18 ± 0.1, respectively. A fourth sole subpopulation was established from randomly selected juveniles to constitute the ungraded control group. Growth of the three subpopulations originally graded according to high (1.04% day⁻¹), medium (0.66% day⁻¹) and low (0.18% day⁻¹) SGR soles, was always similar (P > 0.05) at any time (close to 0.60% day⁻¹). SGR distributions along time revealed a tendency of the three subpopulations to achieve similar growth rate dispersals to that of the ungraded group after 60 days of culture.The individual growth of sole juveniles was also determined in populations that were subject to increased stocking density and food restriction. Soles stocked at high density (N = 110 fish tank⁻¹; 30.97 ± 0.8 kg m⁻²) showed an increased growth standard deviation when compared with individuals kept at low density (N = 20 fish tank⁻¹; 6.20 ± 0.2 kg m⁻²). In contrast, it was found that population growth dispersal was unaffected in soles fed at a daily ration of either 0.25% or 1.0% of their biomass. Minimum consequences on overall size dispersal in terms of ΔCV were found when high stocking and restricted food ration conditions were compared to control groups. Overall results from this work indicate that individual growth of captive juvenile soles was mainly regulated at a population level, existing a strong tendency to achieve a social arrangement in terms of growth. High stocking density and low feeding ration assay suggests that potentially competitive rearing conditions do not contribute to increase population size dispersal during intensive production of S. senegalensis.     

The effect of food type (formulated diet vs. natural) and fish size on feed utilization in common sole,Solea solea (L.)

This study compares the effect of food type (formulated diet vs. natural food) and fish size on protein and energy utilization efficiencies for growth in common sole, Solea solea (L.). Replicate groups of common sole (mean initial body weight ± SD was 45.7 g ± 2.1 and 111.2 g ± 4.2) received the diets at five (natural feed) or four (formulated diet) feeding levels. The protein utilization efficiency for growth (k_gCP) was higher (P > 0.001) in common sole fed ragworms than in common sole fed the formulated diet (respectively, 0.40 and 0.31). Likewise, the energy utilization efficiency for growth (k_gGE) was higher (P = 0.001) in common sole fed ragworms than in common sole fed the formulated diet (respectively, 0.57 and 0.33). The protein maintenance requirement was not different between food types (P = 0.64) or fish size (P = 0.41) being on average 0.82 g kg^?0.8 day^?1. The energy maintenance requirement was not different between food type (P = 0.390) but differed between fish size (P = 0.036). The gross energy maintenance requirement of small common sole was 35 kJ g^?0.8 day^?1. The gross energy maintenance requirement of large common sole was 25 kJ g^?0.8 day^?1. In conclusion, the low growth of common sole fed formulated diets was related to reduced feed utilization.