脱霉剂对种公猪精液品质的影响

饲料霉变及霉菌毒素严重影响饲料工业和畜牧业生产。本试验以种公猪作为试验对象,在公猪日粮中添加G1和G2脱霉剂,试验开始后每周对公猪精液品质进行品质检测,G2见效要比G1快;两种脱霉剂使用后对采精量、每次射精精子总数、精子活力均有所提高,精子畸形率也有下降。综上所述,这两种脱霉剂均可有效提高霉菌毒素引起的公猪生殖性能抑制,从而提高公猪的精液品质,G2效果要更佳。     

阳起石、菟丝子与淫羊藿对肾阳虚大鼠性激素及其受体表达的影响

为了比较阳起石、菟丝子、淫羊藿对肾阳虚模型大鼠的治疗效果,通过肌肉注射氢化可地松制作了肾阳虚大鼠模型,并在3种药物治疗前后分别对体质量、脏器指数、子宫角长度、子宫中段外径、激素水平及其受体基因的表达情况进行了比较研究。结果显示,阳起石、菟丝子、淫羊藿均能够显著增加大鼠的肝脏指数,提高大鼠黄体生成素、卵泡刺激素、雌激素及激素受体的表达水平,其中阳起石与菟丝子对激素分泌的调节优于淫羊藿,而阳起石与淫羊藿对激素受体表达的调节优于菟丝子。结果表明,这3种药物能通过促进激素分泌和激素受体的表达来改善肾阳虚动物子宫和卵巢的功能,且3种药物的疗效各有优势。     

猪冷冻精液的生产及应用

猪精液冷冻保存技术可延长精液的保存时间,适应精液远距离运输,与猪人工授精技术配合应用,可以大幅度降低公猪的饲养数量和引种成本。文章通过综述猪冷冻精液的生产及应用现状,分析影响猪精液冷冻保存和解冻复苏等重要环节的因素。     

种公猪精液伪狂犬病野毒的筛查

本文基于1 100头基础母猪的规模化场进行公猪精液伪狂犬病病毒筛查,农业部规定伪狂犬病病原学检测方法是通过ELSIA方法检测猪伪狂犬病gE野毒抗体。由于抗体检测具有滞后性,导致正在发生感染的猪群g E抗体检测往往呈阴性,但此时通过病原学检测精液已经正在排毒。本文互补了ELSIA抗体检测方法与荧光定性PCR检测病原的优劣,成功实现种公猪精液伪狂犬病病毒的筛查。     

不同稀释液对塞北兔精液保存效果的影响

采用单因子随机分组设计研究了4种不同稀释液对塞北兔精液保存效果的影响.选取同质精液,随机等分为12份,分别用稀释液1、2、3、4按1:1的比例进行稀释.结果表明:稀释液2,即葡萄糖-柠檬酸钠液较其它3种稀释液能明显提高精子的存活时间及生存指数(P<0.01),3、4两种稀释液之间则无明显差异(P>0.05);而稀释液1...     

不同品种公猪精液品质调查

为了研究品种不同的种公猪的精液品质之间存在的差异,本试验通过对长白、大白、杜洛克等3个品种的成年种公猪的精液品质进行统计学方法分析和比较。结果显示:长白公猪的精子活率最好(75.03%),然后依次是大白(71.62%)、杜洛克(70.36%),长白与大白及杜洛克之间差异显著(P<0.05),但大白与杜洛克之间差异不显著(P>0.05);长白种公猪的精液量(337.60mL)与大白(380.00mL)之间差异不显著(P>0.05),但均与杜洛克(251.74mL)之间差异显著(P<0.05)。三个品种精子活率由高到低依次为长白、大白、杜洛克;平均精液量大小依次为大白、长白、杜洛克,综合评价,长白猪的精液品质最好。     

两种牛舍在不同季节对种公牛鲜精品质的影响

本文针对河南省鼎元种牛育种有限公司180头采精种公牛的采精情况,分析两种牛舍(开放牛舍和封闭牛舍)在不同季节对种公牛日平均采精量、密度、活力的影响,以便在生产中采取相应的措施改善种公牛饲养管理条件。结果发现,春、秋两季两种牛舍对种公牛采精量、密度、精子活力的影响无显著差异,但总体上开放式牛舍略优于封闭式牛舍。冬季封闭牛舍的种公牛采精量、精子活力显著高于开放牛舍(P<0.05)。因而认为,春秋季开放牛舍更适合种公牛的饲养,冬季封闭牛舍更利于种公牛精子质量的提高。     

Effects of Egg Yolk Source on the Cryopreservation of Stallion Spermatozoa

Three experiments were conducted to determine whether replacement of chicken egg yolk, as a component of freezing extenders, with egg yolk from other avian species would improve the post-thaw motility and percentage of intact acrosomes of stallion spermatozoa. In the first experiment, substitution of chicken egg yolk with chukar egg yolk, as a component of the lactose-ethylenediaminetetraacetic acid extender, improved (P ≤ .05) the post-thaw motility of stallion spermatozoa. These results were not replicated in (IMV Technologies, Maple Grove, MN, USA) a more expansive study comparing 2%, 4%, 6%, or 8% egg yolk combined with INRA 96 when a “slow freeze” method was used, or the same substitution at levels ranging from 13% to 22% when egg yolk was combined with lactose-ethylenediaminetetraacetic acid for diluents used for a “fast freeze” method of cryopreservation. In the third study, egg yolks from regular and high omega-3 chicken eggs as well as from turkey, chukar, and mallard duck eggs were analyzed for lipid content and fatty acid profile. The yolk from the turkey eggs was higher (1,300 mg/100 g) and that from mallard ducks was lower (560 mg/100 g) in cholesterol as compared with the two types of chicken eggs and chukar egg yolk (range, 1,046-1,094 mg/100 g). In addition, the high omega-3 eggs did test higher for fatty acids (4.51 g/100 g) than other types of eggs (range, 0.28-0.73 g/100 g). Substitution of chicken egg yolk with turkey, but not duck, egg yolk resulted in higher post-thaw total motility (P ≤ .05) for spermatozoa obtained from two of the three stallions used in the third experiment.     

Quality of Raw and of Cold-Stored Semen in Icelandic Stallions

The aim of the present study was to evaluate the quality of raw and cooled semen in Icelandic stallions. Experiments were performed using seven stallions aged between 3 and 19 years. From each stallion, six ejaculates were collected, and semen quality was determined. Thereafter, the semen was split into eight equal parts and processed with and without centrifugation using the extenders INRA 82-egg yolk, INRA 96, GENT, and Equi-Pro to a final concentration of 30 × 10⁶ sperm/mL. The extended semen was then cooled in an Equitainer, where it was stored for 24 hours, and subsequently refrigerated for another 24 hours at 5°C. Immediately after dilution as well as after 24 and 48 hours storage, sperm motility was analyzed using computer-assisted sperm analyzer, and viability was assessed after dual DNA staining with SYBR-14 in combination with propidium iodide. The results show that the stallion had a significant (P < .05) influence on all variables evaluated in raw semen, and mean (±SEM) values of 43.4 ± 4.3 mL for the volume, 193.0 ± 17.0 × 10⁶ sperm/mL for the concentration, 6.7 ± 0.5 × 10⁹ for total sperm and 73.5 ± 2.1% for total sperm motility, 48.7 ± 2.0% for progressive motility, and 65.3 ± 2.0% for rapid cells were measured. In the cold-stored semen, all variables were significantly (P < .05) influenced by the stallion, extender, and storage time (48 hours). Except for Equi-Pro, all extenders examined were suitable for cooled semen preservation. For storage of more than 24 hours, centrifugation and removal of the seminal plasma were advantageous for all extenders with the exception of Equi-Pro.