旱作春小麦籽粒形成与灌浆特性

为了给旱地春小麦高产栽培提供依据,对内蒙古赤峰林东旱作地区春小麦籽粒建成及灌浆特性进行了观察和分析。结果表明,籽粒体积和含水量呈单峰曲线变化,籽粒干物质积累量符合“S”型曲线变化。开花后13-27d是小麦籽粒灌浆直线增长阶段,籽粒含水量相对平稳,籽粒重量直线增长,此期干物质积累量的占粒重的78%,为小麦产量形成的关键时期;不同农艺栽培措施对籽粒形成具有显著影响,只有密度适宜,氯、磷、钾三要素适量配施,才能提高灌浆强度,促进籽粒体积增大,增加粒重,获得高产。     

Effects of Exogenous Materials on Nitrogen Photosynthetic Efficiency and Yield Formation of japonica Rice in Cold Region Under Cold Water Stress at Booting Stage

【Objective】Our goal is to develop disaster-reducing, production-preserving and chemical-controlling cultivation techniques to effectively resist cold damage at the booting stage of japonica rice in cold region.【Method】The split plot design and the central combination test design were used to screen out the optimal application concentrations of CaCl2, glutamic acid(Glu), and γ-aminobutyric acid(GABA) through the membership function values of yield-related indicators under cold-water stress at booting stage.【Result】1) Under cold water stress at the booting stage, the optimal concentrations of exogenous CaCl2, Glu and GABA (CGG) were 1.87 mmol/L, 2.76 mg/L, 4.40 mmol/L, respectively and the average membership function value is the largest (0.932). 2) Compared with normal irrigation, the nitrogen photosynthetic efficiency of cold-region japonica rice decreased under cold stress at the booting stage. Compared with cold water stress at the booting stage, CGG application at the optimal concentration significantly increased the leaf area index (5.37%) and nitrogen photosynthetic efficiency (4.53%) of Dongnong 428 at the full heading stage; significantly increased leaf biomass per leaf area (2.56%) and leaf area index (3.39%) of Songjing 10 at the full heading stage. 3) Compared with cold water stress at the booting stage, the application of the optimal concentration of CGG increased the dry matter accumulation from heading stage to maturity in the cold-region japonica rice, but it was still lower than that of the normal irrigation, and there were differences between varieties and treatments. 4) Compared with cold water stress at booting stage, the yield and yield components of cold-region japonica rice increased with the application of the optimal concentration CGG, and the impact on cold-sensitive varieties was more significant. 5) Compared with the cold water stress at the booting stage, the average increase in temperature production efficiency of Dongnong 428 and Songjing 10 was 5.55% and 23.72% under the optimal concentrations of CGG, respectively. The contribution rate of exogenous CGG to the gap of temperature production efficiency is 8.99%.【Conclusion】The application of the optimal concentration of CGG under cold water stress at the booting stage promoted leaf growth of cold-region japonica rice at the full heading stage and increased the population nitrogen photosynthetic efficiency, which was beneficial to dry matter accumulation and yield formation after heading stage, thereby reducing the loss of cold-region japonica rice under cold water stress at booting stage and improving the temperature production efficiency of cold-region japonica rice.     

高等植物成花的生理生化与分子机制研究进展

成花过程是高等植物生长发育过程中最重要的阶段之一,包括一系列生理生化代谢与基因调控反应。本文对高等植物成花过程中生理生化与分子机制的研究进行了综述,并介绍了miRNA在植物成花过程中的作用,为成花研究提供参考。     

栽培方式对陆两优996产量形成与氮利用效率的影响

在施氮180kg/hm2与不施氮条件下,比较了宽窄行和等行距两种栽培方式对超级杂交早稻陆两优996产量形成特性与氮利用效率的影响。结果表明:(1)与等行距栽培相比,宽窄行栽培具有一定的增产作用,增产原因在于:促进分蘖,提高有效穗数;促进穗分化,提高每穗总粒数;增大叶面积,延缓叶片衰老,促进物质积累;降低茎鞘物质输出率,改善源库关系。(2)与等行距栽培相比,宽窄行栽培的氮素累积量、氮肥利用率与氮素吸收效率显著提高,氮收获指数与氮素利用效率显著下降,而氮生理效率与氮肥效率变化不大。     

提高小麦X玉米杂交产生单倍体胚的频率

Laurie和Bennett（1986）用普通小麦与玉米杂交，并进行幼胚培养，获得了小麦单倍体植株。但要真正用于育种实践，必须首先获得较高并且相对稳定的得胚率。目前报道的单倍体胚诱导频率差异较大，最高的已达48．6％，最低的不足10％（顾坚等，2005）。本实验通过在2，4-D中加入不同体积浓度的二甲基亚砜，研究其对得胚率的影响。     

不同品种水稻产量形成过程的养分积累与分配特征研究

通过测定三系杂交稻"汕优63"、两系杂交稻"两优2186"和常规稻"IR64"不同器官N、P和K含量动态,探讨了3种水稻各生育期的养分积累与分配特征。结果表明:3种水稻稻株N含量均随生长过程逐渐下降,同一生育期水稻N含量以"汕优63"最高;除"汕优63"K含量在齐穗时明显上升外,3种水稻的K含量随生长过程逐渐下降;而P含量总体上在分蘖盛期最高。完熟期3种水稻的N积累量依次为24.79g/m2、15.14g/m2和14.42g/m2,P积累量为4.766g/m2、3.306g/m2和3.678g/m2,K积累量为2.439g/m22、.029g/m2和1.725g/m2。"汕优63"分配到籽粒中的N素比例为61.23%,分别比"两优2186"、"IR64"高17.97%和11.23%;分配到籽粒中的P素比例为62.97%,分别比"两优2186"、"IR64"高6.41%和9.63%;分配到籽粒中的K素比例为27.51%,分别比"两优2186"、"IR64"低3.00%和2.39%。在全生育期中,"汕优63"、"两优2186"和"IR64"的N需求量依次为31.33g/m2、22.88 g/m2、24.82g/m2,N、P、K吸收比依次为1∶0.192∶0.098、1∶0.219∶0.134和1∶0.255∶0.120。3种水稻中"汕优63"的养分生产效率最低。N、P、K生产效率"汕优63"依次为37.25kg/kg、193.80kg/kg和378.80kg/kg,"两优2186"依次为52.54 kg/kg、240.38kg/kg和392.16kg/kg,"IR64"依次为58.38kg/kg、228.83kg/kg和487.80kg/kg。三系杂交稻"汕优63"的N、P、K需求量大,积累量高,分配到籽粒中的比例亦高,与其高产特性相吻合。在保证N、P、K供给的前提下,进一步提高养分生产效率是实现"汕优63"高产、高效的关键。     

Proton toxicity interferes with the screening of common bean (Phaseolus vulgaris L.) genotypes for aluminium resistance in nutrient solution

Common bean (Phaseolus vulgaris L.) proved to be very sensitive of low pH (4.3), with large genotypic differences in proton sensitivity. Therefore, proton toxicity did not allow the screening of common bean genotypes for aluminium (Al) resistance using the established protocol for maize (0.5 mM CaCl₂, 8 μM H₃BO₃, pH 4.3). Increasing the pH to 4.5, the Ca²⁺ concentration to 5 mM, and addition of 0.5 mM KCl fully prevented proton toxicity in 28 tested genotypes and allowed to identify differences in Al resistance using the inhibition of root elongation by 20 μM Al supply for 36 h as parameter of Al injury. As in maize, Al treatment induced callose formation in root apices of common bean. Aluminium‐induced callose formation well reflected the effect of Ca supply on Al sensitivity as revealed by root‐growth inhibition. Aluminum‐induced callose formation in root apices of 28 bean genotypes differing in Al resistance after 36 h Al treatment was positively correlated to Al‐induced inhibition of root elongation and Al contents in the root apices. However, the relationship was less close than previously reported for maize. Also, after 12 h Al treatment, callose formation and Al contents in root apices did not reflect differences in Al resistance between two contrasting genotypes, indicating a different mode of the expression of Al toxicity and regulation of Al resistance in common bean than in maize.     

外源信号物质对肉苁蓉种子萌发与吸器形成POD、CAT活性及ROS含量的影响

利用外源信号物质氟草敏(norflurazon)和2,6-二甲氧基对苯醌(2,6-DMBQ)分别诱导肉苁蓉种子萌发与吸器形成,研究它们在此过程中对过氧化物酶(POD)、过氧化氢酶(CAT)活性及活性氧(ROS)含量的影响。结果表明：在诱导肉苁蓉种子萌发时,经norflurazon处理0~168 h后,种子中POD和CAT活性呈现显著升高的变化趋势,种子中ROS含量显著升高。在诱导肉苁蓉种子萌发体吸器形成时,经2,6-DMBQ处理0~72 h后,肉苁蓉种子萌发体中POD和CAT活性呈现显著升高的变化趋势,萌发体中ROS含量显著升高。表明在诱导肉苁蓉种子萌发与吸器形成过程中,外源信号物质norflurazon和2,6-DMBQ能影响POD、CAT活性及ROS含量的变化。

     

我国亚热带山地土壤发生特性和系统分类研究进展

为了解亚热带山地成土环境下土壤形成规律和分类现状, 推动中西部山地土壤发生学研究和土系建立, 为山地土壤资源的可持续利用提供参考。综述了有关中国亚热带山地土壤的发生发育特性及其系统分类的研究资料, 包括中国亚热带山地的主要地球化学过程和成土过程特点, 亚热带山地土壤分类的主要诊断层、 诊断特性和诊断现象以及高级分类单元的垂直分布规律, 基层分类单元的主要诊断指标。在此基础上, 展望了亚热带山地土壤系统分类研究值得重视的 4个方面：（1）深入理解亚热带山地土壤的地球化学和成土过程;（2） 建立中国亚热带典型山地土壤的代表性土族、 土系, 检验和完善现有基层分类单元的诊断指标体系;（3） 强化山地土壤系统分类中分析指标和数据标准化意识;（4） 重视薄片微形态、 现代仪器分析和计算机新技术的应用。     

泵用压电振子与泵腔体积变化的测试研究

为了准确获得压电泵泵腔体积的变化量,针对影响泵腔体积变化的关键因素——压电振子的变形特性进行分析,并采取非接触的测量方式对基板直径为65 mm,陶瓷直径为60 mm的圆形压电振子进行变形测试.在测试中对该压电振子的中心点在不同电压信号驱动下振幅随频率、驱动电压的变化规律以及压电振子径向各点在正弦电压信号驱动下振幅随半径的变化规律进行了研究.根据压电振子径向各点振幅的变化规律利用Matlab软件进行二次函数曲线拟合计算,得到压电振子的径向切面变形拟合曲线,根据拟合曲线,建立了压电泵泵腔体积变化量的理论计算方法.结果表明：压电振子中心点振幅随频率的增大而逐渐减小,随驱动电压的增大而增大;径向各点振幅随各点半径的增大而减小;拟合计算结果与实测数据基本吻合,最大相对误差仅为6.96%;利用压电泵泵腔体积变化量的理论计算方法,计算得到利用该圆形压电单晶片振子制作的压电泵在100 V,30 Hz正弦电压信号的作用下,泵腔每次振动产生的体积变化量约为34.009 mm3,理论上最大输出流量为61.216 mL/min.