应用生物素标记的NDV－cDNA探针检测感染尿囊液中NDV－RNA的初步研究

本文应用聚合酶链反应（ＰＣＲ）技术从构建的新城疫病毒（ＮＤＶ）ｃＤＮＡ文库中扩增含编码Ｆ糖蛋白前体──Ｆｏ酶切位点序列的３５９ｂｐ的Ｆ蛋白基因ｃＤＮＡ片段。将此３５９ｂｐｃＤＮＡ片段经光敏生物素标记后,即成ＮＤＶ－ｃＤＮＡ探针。该探针能特异性地从感染的尿囊液中检测出ＮＤＶ强毒株和疫苗毒株的基因组ＲＮＡ,而不与ＩＢＤｖ－ｄｓＲＮＡ、ＡＩＢｖ－ｓｓＲＮＡ、ＥＤＳ７６－ｄｓＤＮＡ、ＭＤＶ－ｄｓＤＮＡ,ＦＰＶ－ｄｓＲＮＡ及ＡＩＬＶ－ｄｓＤＮＡ发生交叉杂交反应。试验结果表明：尽管该探钎含有编码Ｆｏ蛋白酶切位点序列的碱基顺序,但它还是不能把ＮＤＶ的强、弱毒株区分开。这说明ＮＤＶ强、弱毒株比区域内的碱基存在着相当大的同源性。不过,此探针对ＮＤＶ来说具有特异性,这就为ＮＤＶ的诊断技术开创了基因水平检测的新途径。     

2型猪链球菌的血清学鉴定

本试验首次证实了２型猪链球菌在我国的存在，采用玻片凝集、琼脂扩散及毛细管沉淀等３项试验，对１９９０年以来广东某猪场断奶仔猪暴发流行的败血症、脑膜炎及关节炎病猪中分离的１５株链球菌进行了血清学鉴定。结果表明：１５株链球菌均为２型猪链球菌；用高压法提取抗原进行沉淀反应，其结果比酸热法提取抗原具有简便、敏感、特异性强的特点。     

Unsaturated vitamin b(12) binding capacity in human and ruminant blood serum - a comparison of techniques including a new technique by high performance liquid chromatography

A new technique by High Performance Liquid Chromatography (HPLC-gel permeation) shows promise as a tool to separate and quantitate the Unsaturated Vitamin B(12) Binding Capacity (UBSC) of the individual Vitamin B(12) binders in blood serum. This method, although not as rapid as protein-coated charcoal or cellulose separation techniques, is more applicable for use with large numbers of samples than gel filtration. The use of a radioactivity detector to monitor the eluant from the column permitted automation of the method. Comparable results for UBBC and for the UBBC of individual binders were obtained when samples were analyzed by gel filtration and HPLC. The HPLC method proved suitably precise and the recovery of added cyanocobalamin was acceptable. It is proposed that HPLC be the method of choice for measurement of the USBC of binders of Vitamin B(12) in blood serum.     

柞树害虫栎粉舟蛾的初步研究

栎粉舟蛾Femtoma ocypete(Bremer)是柞树的重要害虫之一。此虫在辽宁一年发生一代,以蛹在土中越冬。翌年7月中旬开始羽化,持续到8月中旬。羽化后即可交配产卵。7月下旬到8月下旬均有幼虫孵出。幼虫经6个龄期,于9月中、下旬入土化蛹。防治方法:8月中旬往柞树上喷洒0.025％浓度的“杀虫灵”农药,能杀死各龄幼虫,防治效果为90％左右,七天后养蚕无害。     

苎麻自交一代分离和变异的研究

1983～1985年以“黄壳早”、“芦竹青”、“古巴苎麻”和“武冈红皮种”为材料进行自交,并观测了自交一代的分离和变异情况,研究结果表明:a.根据自交一代的形态分类,可将“芦竹青”繁殖后代分为40个类型,“芦竹青”自交一代的麻骨颜色分离为四种,其分离比例为9.3:4.2:3:0.7,基本符合孟德尔遗传规律;b.个体间产量性状差异很大,株高变冥为0.23～1.68m,茎粗变异为0.48～1.14 cm,有效株变异为1～23株;c.40个类型的纤维细度的变异呈正态分布,变异为1168～1905公支,最细的类型和最粗的类型相差747支上述结果表明:苎麻自交后代的形态性状、产量性状和纤维细度均产生显著的分离和差异。     

光敏核不育水稻选育与利用的几个问题讨论

对现有光敏核不育材料进行了分类,分为４种遗传类型;其中,温光弱感到光敏核不育系是最为理 想的遗传工具,并就其选育途径和选择“粳不籼恢”繁殖制种技术体系进行了论证．     

THE CLIMATE CHANGE AND FORESTRY OF HEILONGJI ANG PROVINCE IN 1990''''S

INTRODUCTIONIntheworldoftoday,theglobalclimatechangeanditsinfluenceonecologyhavebe-comeaveryimportantproblem,towhichmanyscientists,governnentleadersandordinarypcoplepaycloseattentionI1-'].Inl979,theWorldClimateResearchProgram(WCP)waslaiddowninthefirstworldclimatemeeting.lnl99(),thesecondworldclimatemeetingwasconvcl1edinGencva,andalltl1eexpertsagreedthattheglobalwarmlngwillbeextremelyseriousdisasterthananynatUralcalamityever.Attl1eMectingof"WorldEnvironmentandDevelopment,"holdinBrazil…     

Dynamics ofRhizoctonia solani (black scurf) in successive potato crops

The position of plants withRhizoctonia solani sclerotia (black scurf) on progeny tubers was mapped for an experimental field at Haren where potatoes were grown continuously and in rotation with other crops for five successive years, and for another field at Borgercompagnie with a 12 frequency of potatoes during three potato crops. Initially, the distribution of plants with black scurf on both fields was rather dense and homogeneous. In the following years the distribution became heterogeneous and patchy. The local decline ofR. solani AG 3 (the common potato pathogen) in Haren was apparently caused by an unknown factor selectively suppressingR. solani AG 3, while simultaneouslyR. solani AG 5 increased in mass. This AG 5 type proved to be an inferior competitor of AG 3 on the potato plant in a laboratory experiment. The specificR. solani antagonistVerticillium biguttatum did not play a role. A similar factor could have reduced the formation of black scurf in the experimental field at Borgercompagnie, whereV. biguttatum was also too infrequent to account for the decline.R. solani AG 5 was not present here and could not indicate the presence of a selective factor against AG 3.     

Identification of feline blood B and T lymphocytes by cell electrophoresis.

The electrokinetic properties of feline blood lymphocytes isolated by centrifugation over Ficoll-Isopaque gradient were investigated. A biphasic electrophoretic mobility (EPM) distribution was regularly observed with a low-mobility (LM) population (mean EPM: 0.82) accounting for 32% of blood lymphocytes and a high-mobility (HM) population (mean EPM: 1.09) representing 68% of blood lymphocytes. Following fractionation on nylon-wool columns, lymphocytes with B-cell properties (64% sIg⁺; 9% guinea pig erythrocytes (GPE)-rosette⁺, PHA and Con A unresponsive) were enriched in the adherent fraction and belonged mainly (78%) to the LM population. In contrast, lymphocytes with T properties (5% sIg⁺, 42% GPE-rosette⁺, PHA and Con A responsive) were recovered in the effluent fraction and comprised 84% of HM elements.Thus, in cat blood, LM lymphocytes are likely to represent in majority B cells and HM lymphocytes T cells. This indicates that cell electrophoresis provides an interesting mean for differentiating B and T cells in the cat.