三斑圆雀鲷的形态结构研究

在活体解剖三斑圆雀鲷的基础上,系统描述了其外部形态特征和内部构造。三斑圆雀鲷鱼体侧扁,体被栉鳞;头小眼大,侧线不连续;背鳍、臀鳍、尾鳍基部具有鳞鞘;鱼体呈褐色到黑色,具有3个白点;背鳍硬棘12支,臀鳍具硬棘,尾鳍形;胃发达呈圆形;具有3根粗大的幽门盲囊;雌雄异体,生殖腺紧贴于膘的腹面。     

哺乳动物腔前卵泡体外培养及研究进展

哺乳动物卵巢内的原始卵泡库是雌性生殖资源的储存库,但大部分卵泡在腔前阶段已退化闭锁了,这无疑是对这一资源的巨大浪费。因此,越来越多的学者致力于腔前卵泡的开发和利用,建立了一系列的培养体系,尤其在小鼠上做的比较成功,已经得到了少量后代。文章主要就卵泡的发生发育过程,腔前卵泡的分离方法,培养基的选择,不同直径腔前卵泡培养体系的建立,影响腔前卵泡发育的因素及研究进展进行了阐述,为进一步完善腔前卵泡培养体系,揭示其发育机理提供参考。     

海产品中牛磺酸测定前处理方法探讨

探讨了海产品中牛磺酸测定的三种前处理方法:6%磺基水杨酸热水浴提取法;0.02mol/L盐酸提取法;70%乙醇沸水浴提取法。结果表明:采用6%磺基水杨酸热水浴提取法均比0.02mol/L盐酸提取法和70%乙醇沸水浴提取法测定值高,测定结果精确度高、重现性好、回收率达98%、变异系数(CV)为1.86%。该法试剂成本低,操作简便,且能同时测定其它18种游离氨基酸,是海产品牛黄酸测定的理想方法。     

2004～2008年《海洋水产研究》研究生论文质量分析

以2004～2008年《海洋水产研究》发表研究生论文为统计对象,运用文献计量学的方法对载文量、发表时滞、作者群进行分析,并对今后工作提出建议。     

辽东湾4种海洋生物的热耐受性研究

本研究采用动态温升实验方法,以矛尾虎鱼(Chaeturichthys stigmatias)、许氏平鲉(Sebastes schlegeli)、口虾蛄(Oratosquilla oratoria)和日本蟳(Charybdis japonica)为研究对象,研究了5个基础水温(8.0、14.0、18.0、24.0和28.0℃)和9个温升速率(0.5、1.0、2.0、3.0、4.0、6.0、9.0、12.0和15.0℃/h)下4种海洋生物的半致死温度(Lethal temperature of 50%,LT_(50))。结果显示,基础水温和温升速率均能显著(P0.05)影响实验生物的LT_(50)。特定温升速率下实验生物LT_(50)与基础水温呈正相关,即各个实验生物的LT_(50)随着基础水温的升高而升高。温升速率对实验生物LT_(50)的影响因基础水温和物种而异。本研究在动态温升实验中结合使用不同基础水温和多个温升速率,构建了海洋生物重要的热耐受性评价指标LT_(50),可广泛应用于海洋生物的热耐受性评价。     

A survey of antimicrobial-resistant Escherichia coli prevalence in wild mammals in Japan using antimicrobial-containing media

The emergence and spread of antimicrobial-resistant bacteria and resistance genes pose serious human and animal health concerns. Therefore, to control antimicrobial-resistant bacteria in the environment, the status of antimicrobial resistance of Escherichia coli in a variety of wild mammals and their prevalence were examined using antimicrobial-containing media. In total, 750 isolates were obtained from 274/366 (74.9%) wild mammals, and antimicrobial-resistant E. coli was detected in 37/750 isolates (4.9%) from 7 animal species (26/366 [7.1%] individuals). Using antimicrobial-containing media, 14 cefotaxime (CTX)- and 35 nalidixic acid-resistant isolates were obtained from 5 (1.4%) and 17 (4.6%) individuals, respectively. CTX-resistant isolates carried bla_CTX-M-27, bla_CTX-M-55, bla_CTX-M-1, and bla_CMY-2, with multiple resistance genes. Fluoroquinolone-resistant isolates had multiple mutations in the quinolone-resistance determining regions of gyrA and parC or qnrB19. Most resistant isolates exhibited resistance to multiple antimicrobials. The prevalence of antimicrobial-resistant bacteria observed in wild mammals was low; however, it is essential to elucidate the causative factors related to the low prevalence and transmission route of antimicrobial-resistant bacteria/resistance genes released from human activities to wild animals and prevent an increase in their frequency.     

消费者视角下特色农产品品牌形象研究 ——以烟台海参为例

以海参消费者为调查对象,采用设计调查问卷以及实地考察的方法进行调研。研究发现：烟台海参企业的品牌知名度不高,海产品市场为购买海参的主要途径,购买的产品种类主要为淡干海参,用途以送父母为主,获取海参信息的方式有传统媒体、新媒体及亲朋推荐,消费者购买海参时最看重的是质量;烟台海参品牌形象测量指标可以提取为三个主要因子：企业形象因子、产品形象因子和产地形象因子。最后,从企业层面、产品层面和产地层面提出烟台特色农产品品牌建设的建议。     

Unusual External Adaptations in the Rock Hyrax

Activity in two unrelated genera of mole-rats, Tachyoryetes and Heliophobius, was studied in the field by recording the movements of animals tagged with radioactive wire. Tachyoryetes shows a single marked activity peak and only leaves its nest between 10.00 and 19.00 hour. Heliophobius shows a more dispersed and prolonged activity pattern although peak activity occurs over approximately the same period as in Tachyoryetes. Heliophobius spends over 50 of the day out of its nest, Tachyoryetes, under 25%.

These differences can be attributed to a different function of the nest in the two genera (Tachyoryetes has a multipurpose nest; Heliophobius uses its nest solely for rest), and also to the fact that Tachyoryetes has light-sensitive eyes whereas Heliophobius appears unable to appreciate light; Tachyoryetes periodically comes to the surface to forage and this exposure to light may trigger the 24-hour activity cycle.     

海洋细菌AiL3菌株防治香蕉枯萎病研究

采用室内盆栽和大田小区试验对海洋细菌AiL3菌株防治香蕉枯萎病作用及对香蕉的促生效果进行测定.结果表明:菌株处理后盆栽试验和大田试验中的香蕉苗叶片数、株高、光合速率和蒸腾速率等均明显高于对照;AiL3菌株盆栽试验处理6个月后对香蕉枯萎病的防治效果达100％,大田小区试验菌株处理8个月后对香蕉枯萎病的防治效果分别为72.71％和46.32％.表明AiL3菌株对香蕉植株有一定的防病促生作用.     

海藻研究和成果应用综述

介绍了海藻的分类,综述了海藻在农业、能源、医学、食品保健、环境保护、光合机制机理方面的研究及应用,指出了我国藻研究仍然存在的问题并展望了今后的研究方向。