西尼罗热病毒RT-PCR检测方法的建立

参考Genebank发表的西尼罗热病毒(West Nile virus，WNV)E糖蛋白基因序列，自行设计合成一对引物，对WNV进行RT—PCR扩增，产物经琼脂糖电泳分析，呈现一条约400bp的条带，将其克隆入pMD18-T—Vector载体中，并进行序列测定，与已发表的WNV基因比较发现，核苷酸的同源性为99．7％，证实为WNV的E基因，通过对样品多次检测，都能扩增出一条约400bp的条带，表明该方法比较稳定。     

菜籽油裂解燃料离子液体催化酯化反应降酸工艺的研究

为了降低裂解燃料酸值,增加燃料的稳定性能,将吡啶丁烷磺酸硫酸氢盐应用于菜籽油裂解燃料的酯化反应。考察了催化剂用量、反应时间和反应温度等对酸值的影响,并在最佳优化条件下考察了裂解燃料成品的理化性质。结果表明:吡啶丁烷磺酸硫酸氢盐对催化酯化反应具有很高的催化活性。优化工艺条件为:催化剂用量1.2%、反应温度75℃、反应时间70 min,在此工艺条件下,酸值降低到1.0 mgKOH/g以下。     

烟草氨基酸代谢及其调控机制

综述了植物氨同化以及烟草中苯丙氨酸代谢、脯氨酸代谢、美拉德反应及其与烟叶风味和品质关系的研究进展,并分析了环境因素对烟草氨基酸代谢的影响,为深入研究氨基酸对烟叶品质和风味影响的机制奠定了基础。     

东京野茉莉花色成分的初步研究

对东京野茉莉花色成分进行了初步的探究。结果表明:通过特征颜色反应和光谱扫描说明了其花瓣细胞内有异黄酮、儿茶精类,还有叶绿素a、b,可能含有胡萝卜素、黄酮类化合物,以及无色花色素,可能不含查耳酮或橙酮。     

Detection of Ureaplasma diversum in the upper airways of Australian feedlot cattle

Bovine respiratory disease (BRD) exerts a major impact on the beef cattle industry nationally and worldwide, with a range of aetiological factors impacting its pathogenesis. Previous research has focussed on an increasing number of bacteria and viruses that have been shown to play a role in eliciting disease. Recently, additional agents have been emerging as potential contributors to BRD, including the opportunistic pathogen Ureaplasma diversum. To determine if U. diversum was present in Australian feedlot cattle and if that presence was linked to BRD, nasal swabs were collected from a cohort of 34 hospital pen animals and compared to 216 apparently healthy animals sampled contemporaneously at feedlot induction and again after 14 days on feed at an Australian feedlot. All samples were subjected to a de novo polymerase chain reaction (PCR) assay targeting U. diversum in combination with other BRD agents. U. diversum was detected at a low prevalence in cattle at induction (Day 0: 6.9%, Day 14: 9.7%), but in a significantly greater proportion of cattle sampled from the hospital pen (58.8%). When considering the presence of other BRD-associated agents, co-detection of U. diversum and Mycoplasma bovis was most common in hospital pen animals receiving treatment for BRD. These findings suggest that U. diversum may be an opportunistic pathogen involved in the aetiology of BRD in Australian feedlot cattle, in combination with other agents, with further studies are warranted to identify if a causal relationship exists.     

接种疫苗的健康风险研究进展

在广泛文献检索的基础上,对疫苗的历史、种类、防腐剂、不良反应和安全接种等进行了综述,为接种疫苗的健康风险研究提供科学资料。     

抗滑桩优化设计在滑坡治理中的应用研究

基于传统剩余推力法，提出了新的抗滑桩优化方法。以位于凉山彝族自治州某县城南的滑坡体为例，说明了主要治理方法。采用剩余推力法和新的抗滑桩优化方对该滑坡体天然状态工况进行了稳定分析。结果显示，在设计安全系数为1．15的情况下，该滑坡处于临滑状态，因此，须采取相应的加固措施，最后推荐了抗滑桩设计方案，已在凉山州某县城滑坡工程中成功实施。