In this study, we determined the cause of a disease outbreak in spotted sea bass, Lateolabrax maculatus reared in culture cages on the western coast of Korea in 2013. The major signs in the diseased fish exhibited were haemorrhaging on the membranes of the abdomen, gastrointestinal organs and opercular gills, as well as an enlarged spleen. No external morphological signs of infection were visible, except for a darkening in colour. No parasites or pathological bacteria were isolated from the diseased fish; however, epithelioma papulosum cyprini (EPC) cells inoculated with tissue homogenates from the diseased fish showed cytopathic effects (CPEs). Virus particles in the EPC cells were bullet‐shaped, 185–225 nm long and 70–80 nm wide, characteristic of Rhabdoviridae. Polymerase chain reaction analyses of homogenized tissues from the diseased fish and supernatants of cell cultures with CPEs indicated specific, 553‐bp‐long fragments corresponding to the matrix protein gene of the hirame rhabdovirus (HIRRV). Phylogenetically, the HIRRV phosphoprotein gene of spotted sea bass was more closely related to phosphoproteins from Chinese and Polish HIRRV strains than from other Korean strains. To our knowledge, this is the first report of HIRRV infection in cultured spotted sea bass. 相似文献
实验以鸡肉粉∶牛肉骨粉∶喷雾血球干燥粉∶水解羽毛粉=40∶35∶20∶5的比例组成的混合动物蛋白(animal protein blend,APB)分别直接替代或在补充赖氨酸、蛋氨酸、苏氨酸后替代花鲈饲料中50%、75%、100%的低温干燥鱼粉,研究其对花鲈生长及生理功能的影响。经过8周的饲养后发现,花鲈的生长性能随替代比例的提高而降低;摄食含APB饲料的花鲈,其成活率,摄食率和生长性能均显著低于对照组(P<0.05)。与APB直接替代鱼粉的处理组相比,添加3种必需晶体氨基酸极显著提高了花鲈的生长性能及全鱼蛋白、脂肪、能量含量(P<0.01),显著降低了花鲈的全鱼水分、灰分,肝指数及脏体比(P<0.05)。晶体氨基酸与APB的替代水平对花鲈的生长性能、形体指标及体组成成分存在显著的交互作用(P<0.05)。实验结果表明,(1)混合动物蛋白替代初始体重为13.2 g的花鲈饲料中白鱼粉的比例应小于50%;(2)添加必需晶体氨基酸能显著提高APB在花鲈饲料中的应用潜力;(3)花鲈具备一定的利用晶体氨基酸的能力。 相似文献