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Effects of single and combined microalgae on larval growth, development and survival of the commercial sea cucumber Holothuria spinifera Theel 总被引:1,自引:0,他引:1
The results of the study on the suitable algal feed for the mass rearing of holothurian larvae through hatchery system are presented. Auricularia larvae, after 48 h of fertilization, obtained from induced spawning of Holothuria spinifera, were fed with different algae Isochrysis galbana, Nanochloropsis salina, Pavlova lutheri, Tetraselmis chuii and Chaetoceros calcitrans as well as I. galbana+C. calcitrans to ascertain the effect of single and combined microalgal diet. The rate of feeding was 2 × 104 cells larvae–1 day–1 for a period of 9–12 days. The growth rate of 59 μm day−1 with 90% and 43 μm day−1 with 100% occurrence of late auricularia in the larvae fed with C. calcitrans alone and I. galbana+C. calcitrans, respectively, indicated that C.calcitrans itself or in combination with I. galbana is the effective feed for the larvae of H. spinifera. 相似文献
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激光辐射对两种饵料单胞藻的活化与复壮作用 总被引:3,自引:0,他引:3
单细胞藻类是鱼、虾、贝类等苗种生产的基础性活饵料,随着水产养殖业的快速发展,高产优质饵料藻种的选育已成为规模化人工育苗过程的关键性环节。目前,在微藻生物饵料的生产过程中,藻种常因种质衰退而出现生长缓慢,产量降低,抗逆性减弱等问题,因而造成饵料供应的严重不足。而应用传统方法进行藻种复壮或重新选育优良藻株的周期比较长且需要花费大量的人力。[第一段] 相似文献
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通过考察乙酰甲喹对小新月菱形藻(Nitzschia closterium f.minutissima)、等鞭金藻(Isochrysis galbana Parke3011)的生长抑制和对叶绿素a、总超氧岐化酶(T-SOD)活性以及丙二醛(MDA)浓度的影响,研究了乙酰甲喹对2种微藻的毒性效应,为评价兽药乙酰甲喹的使用安全性和保护养殖环境提供基础数据。结果表明,乙酰甲喹对2种微藻的生长抑制作用随质量浓度的增加而增大,乙酰甲喹对小新月菱形藻的24 h-EC50(半数有效浓度)为4.73 mg·L~(-1)、对等鞭金藻3011的24 h-EC50为2.14 mg·L~(-1),乙酰甲喹对2种微藻均属于高毒物质,等鞭金藻3011更敏感。暴露24 h后,随着乙酰甲喹质量浓度的升高,小新月菱形藻叶绿素a浓度下降而等鞭金藻3011叶绿素a浓度基本不受影响;2种微藻的T-SOD活性随着乙酰甲喹质量浓度的升高均显著增加,各实验组MDA浓度也高于对照组,等鞭金藻3011更敏感,说明乙酰甲喹对2种微藻均能造成氧化胁迫,对细胞造成氧化损伤。 相似文献
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在水温(22±1)℃和连续光照强度为50μmol·m~(-1)·s~(-2)下,取主要由乳酸菌、硝化菌、芽孢杆菌、光合菌,及酵母菌等组成的培藻肥水型EM菌0.5m L、2.5m L或5.0m L,分别加入到200m L密度相同的小新月菱形藻Nitzschia closterium f.、球等鞭金藻3011 Isochrysis galbana parke和小球藻Chlorella sp.中培养15d,以f培养基为对照,研究不同浓度培藻肥水型EM菌对这3种藻类生长的影响。结果表明:EM菌对金藻、硅藻、绿藻的生长都有一定的促进作用,其中对金藻及硅藻生长的促进作用更加显著,生长率提高20%~50%,最终细胞密度有所提高,添加5m L促生长作用更持久。 相似文献
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对耐低温品系球等鞭金藻8701在低温下与活性氧清除和伤害有关的几个生理生化指标进行了研究,探讨了其耐低温机理,并与该藻种的低温敏感品系球等鞭金藻3011进行了比较,结果表明,(1)球等鞭金藻8701在低温下(8℃)培养一周,其超氧化物歧化酶(SOD)活性略有下降,但仍保持极高的酶活性,因此能够及时清除低温胁迫产生的过量活性氧,使其细胞内保持较低的膜脂过氧化水平和较低的电解质外渗率,(2)球等鞭金藻3011在低温下胁迫一周,其细胞内的膜脂过氧化水平和电解质在率大大高于球等鞭金藻8701,这与其SOD活性较低,并且在低温下其活性大幅度下降有关,(3)与DOD相比,两种不同品系球等鞭金藻的过氧化物酶(POD)对减轻其低温伤害,提高其耐低能力,不起主要作用。 相似文献
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Xiuyuan Ran Yuhan Shen Dongjian Jiang Chenqi Wang Xinghui Li Haoyu Zhang Yunyun Pan Chenglin Xie Tonghui Xie Yongkui Zhang Changhong Yao 《Marine drugs》2022,20(6)
Chrysolaminarin, a kind of water-soluble bioactive β-glucan produced by certain microalgae, is a potential candidate for food/pharmaceutical applications. This study identified a marine microalga Isochrysis zhangjiangensis, in which chrysolaminarin production was investigated via nutrient (nitrogen, phosphorus, or sulfur) deprivations (-N, -P, or -S conditions) along with an increase in light intensity. A characterization of the antioxidant activities of the chrysolaminarin produced under each condition was also conducted. The results showed that nutrient deprivation caused a significant increase in chrysolaminarin accumulation, though this was accompanied by diminished biomass production and photosynthetic activity. -S was the best strategy to induce chrysolaminarin accumulation. An increase in light intensity from 80 (LL) to 150 (HL) µE·m−2·s−1 further enhanced chrysolaminarin production. Compared with -N, -S caused more suitable stress and reduced carbon allocation toward neutral lipid production, which enabled a higher chrysolaminarin accumulation capacity. The highest chrysolaminarin content and concentration reached 41.7% of dry weight (%DW) and 632.2 mg/L, respectively, under HL-S, with a corresponding productivity of 155.1 mg/L/day achieved, which exceeds most of the photoautotrophic microalgae previously reported. The chrysolaminarin produced under HL-N (Iz-N) had a relatively competitive hydroxyl radical scavenging activity at low concentrations, while the chrysolaminarin produced under HL-S (Iz-S) exhibited an overall better activity, comparable to the commercial yeast β-glucan, demonstrating I. zhangjiangensis as a promising bioactive chrysolaminarin producer from CO2. 相似文献
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用温度梯度法、二因子重复试验法分别研究温度、光照周期和光照强度对绿色巴夫藻的生长、总脂肪含量以及脂肪酸组成的影响,用正交试验法研究以上三因子对等鞭藻3011的影响。结果表明,温度20℃、光照强度120.19μmol/(m2·s)、光照周期L:D=16:8最适于等鞭藻3011的生长及脂肪和PUFA的合成。绿色巴夫藻适宜在15~30℃内生长,但在20℃、9615μmol/(m2·S)及L:D=18:6的条件下,能促使它较快生长且能合成较多的脂肪和必需脂肪酸。两种微藻ω3/ω6显著性地受到环境因子的综合影响。 相似文献