不同温度下皱纹盘鲍"中国红"与各家系代谢和吸收效率的比较

为了综合评价皱纹盘鲍Haliotis discus hannai红壳色新品系"中国红"(RwRh) 的生理能量学特性,采用实验生态学的方法比较了12、15、18、21、24 ℃条件下,RwRh与皱纹盘鲍不同地理群体(J1Jm、C1Jm、C1Rh、J7Rh和RjRh)的代谢率和吸收效率.结果表明: J1Jm(皱纹盘鲍日本群体♀×日本群体♂)和C1Jm(皱纹盘鲍中国群体♀×日本群体♂)的耗氧率随水温的升高而上升,但超过18 ℃在21 ℃下耗氧率明显下降,RwRh与其半同胞家系(C1Rh、J7Rh、RjRh)的耗氧率拐点出现在21 ℃,说明"中国红"及其半同胞家系更适宜较高温度;各家系排氨率随温度升高的变化趋势与耗氧率基本相同.方差分析显示,在12～24 ℃内,家系对吸收效率的影响未达到显著水平,但在21～24 ℃下"中国红"有较高的吸收效率.     

Reproduction strategy of nocturnal marine molluscs: running for love

The cost of reproduction is the core driver of life history evolution in animals. This paper demonstrates that the cumulative distance moved and the duration of movement of sexually immature abalones, Haliotis discus hannai, kept in various male and female groups, were significantly higher than those of sexually mature individuals, except when kept in mixed cultures of mature males and females. After mixed-culture, sexually mature males moved significantly further and for a longer duration than mature female abalones, and even more so than mature male abalones of any other group. Examination of the LC-MS metabolomics of mature males cultured with sexually mature females (AM) and those cultured with sexually immature females (JM) showed that cyclic adenosine monophosphate (cAMP) acted as a differential metabolic biomarker. After 24-h uninterrupted sampling, the concentration of 5-HT and the expression levels of the 5-HT2 and 5-HT6 receptors in AM were significantly higher than those in JM. After further injection of 5-HT2 and 5-HT6 receptor antagonists, the concentrations of cAMP and PKA rose again, but the cumulative movement duration and distance of male abalones decreased significantly, showing that 5-HT was involved in the regulation of movement behavior of male abalones through the 5-HT2 and 5-HT6 receptor-activated cAMP-PKA pathways. The results demonstrated a significant increase in the movement endurance of mature male abalones cultured with mature females, providing a theoretical basis for understanding the adaptive life history strategies of abalones and suggesting ways to protect diverse benthic resources for abalones during the reproductive stage.