制品生产用及地方疑难布氏菌DNA基因同源性的研究

本文以布氏菌19株国际标准菌种为对照,对国内外实验室参考菌种14株及地方疑难菌种11株结合常规方法进行了DNA G+Cmol％测定,同时以羊种菌M16 DNA为标准,与布氏菌所有种和型的24个代表株及8株地方疑难菌种进行了DNA-DNA杂交测定,布氏菌及地方疑难菌种的G+Cmol％值范围为54.1～59.3,M16株与布氏菌参考菌种的杂交率81.7～109％,与地方疑难菌种的杂交率除一株为72.1外,其余为84.2～109％,属高度同源,鉴定为布氏菌。本试验首次使用进口新仪器“PEKIN LAMBDA 17UV/Vis SPECTROPHOTOMETER”采用热变性法测DNA G+Cmol％,复性速率法进行DNA-DNA杂交,使杂交的DNA样品变性和复性在同一比色杯中进行,省去半导体点温计操作带来的麻烦,减小了实验误差,缩短测时,结果精确、操作简便、重复性好。     

早熟合抱类型大白菜龙白6号的选育

龙白6号大白菜为早熟合抱类型一代杂种。亲本N-30是由地方品种二牛心连续自交4代后选出的自交不亲和系,亲本00-020是从龙白1号中经连续自交分离育成的自交不亲和系。龙白6号为矮桩牛心形,合抱,花心,外叶绿色,心叶淡黄色,球形指数1. 49,软叶较多,净菜率76 %左右,单株质量4. 0 ~4. 5kg,品质好。高抗病毒病,抗霜霉病,平均产量100t·hm-2左右,适于黑龙江省各地秋季露地栽培。     

Management ofMeloidogyne incognita inArtemisia pallens with bio-organics

The sedentary endoparasitic root-knot nematode [RKN],Meloidogyne incognita (Kofoid and White) Chitwood, is worldwide an economically important agri-pest, reducing the yield and quality of crops. The traditional method of RKN control is based mainly on chemical nematicides, which will not be frequently available from 2005 onwards; their use is highly objectionable due to their major contribution to ground water contamination, and dangers to the environment and to human and animal health. Consequently, new and environmentally safe tools for RKN management are urgently needed. The objective of the present investigation was to evaluate different bio-organic wastes on RKN infestation and the growth / oil yield ofArtemisia pallens Wall. The tested wastes were: 1. Distillation waste ofMentha arvensis (menthol mint); 2.Murraya koengii (meethi neem / curry leaf); 3.Cymbopogon flexuosus (lemongrass); 4.C. martinii (palmarosa); 5.C. winterianus (citronella); 6.Pelargonium graveolens (geranium); 7.Pogostemon patchouli (patchouli); 8.Tagetes minuta (marigold); 9. Vermicompost ofM. arvensis, Chrysanthemum cinerariaefolium (pyrethrum),Tagetes minuta andC. winterianus; 10.Trichoderma harzianum isolate U; 11. VA fungiGlomus aggregatum; G. fasciculatum. The results obtained with these different bio-organics were compared with three controls: untreated — uninoculated, untreated — inoculated and the chemical pesticide carbofuran. Results revealed a considerable enhancement of herbage biomass, flower bud and oil yields when plants were treated with the distillation waste ofC. martinii, C. winterianus, M. koengii, M. arvensis, vermicompost ofC. cinerariaefolium, T. minuta, M. arvensis and bio-agentT. harzianum. Least nematode infections were recorded with the distillation waste ofM. koengii, C. martinii, C. flexuosus, and vermicompost ofT. minuta, C. cinerariaefolium andM. arvensis. The results revealed that these environmentally sound bio-organics could be used for replacement of chemical nematicides. http://www.phytoparasitica.org posting May 20, 2005. The work was conducted at CIMAP-CSIR, Lucknow, India.     

6-BA对杨树菇液体培养的影响研究

适宜浓度的6-BA应用于杨树菇液体培养，可明显促进杨树菇菌丝生长和可溶性糖的产生。适宜的6-BA作用浓度为0．15mg／L，它使菌丝体的产量和可溶性糖含量分别提高了17．3％和15.0％。     

野生与栽培灵芝挥发油成分的分析

将野生灵芝和栽培灵芝依次用乙醚、甲醇回法提取，对醇，醚提取物用蒸馏法提取挥发油，利用GC－MS技术对挥发油进行分析，结果表明：野生灵芝和栽培灵芝挥发油的种类分别为22种和10种；在测得的野生灵芝的22种挥发油中，脂肪酸类化合物居多，其中含量最高的是十六碳酸，占挥发油总量的50．68％。     

6-BA对葡萄果实生长及碳、氮同化物运输的影响

 研究了6-BA 对葡萄果实生长及碳、氮同化物运输、分配的影响。结果表明, 果穗浸蘸100 或200 mg/L 6-BA , 均可明显提高坐果率, 对果粒大小、可溶性固形物含量影响较小。6-BA 处理¹⁴CO₂饲喂叶,抑制了饲喂叶¹⁴CO₂同化物的输出, 但6-BA 处理饲喂叶上方正在生长的“库叶”, 则增加了饲喂叶同化物的输出。62BA 在盛花和盛花后10 d 浸蘸花序2 次, 增加了饲喂叶¹⁴CO₂ 、¹⁴C - 谷氨酸(¹⁴C₂Glu) 向穗轴及果实的输入。     

检测结核分枝杆菌CFP10-ESAT-6分泌蛋白双抗体夹心ELISA的建立

用重组蛋白CFP10-ESAT-6免疫新西兰大白兔,通过ELISA方法检测抗体效价水平;高效价抗体用DEAE-32阴离子交换柱纯化后,一部分抗体为包被所用,另一部分抗体用辣根过氧化物酶标记后,作为检测用.建立双抗体夹心法,进行了初步鉴定,确定该方法的可行性.结果表明:抗CFP10-ESAT-6多克隆抗体的效价在1:16 000稀释后D150值基本都达到1.0左右,在纯化、酶标记后,分别以1 : 5 000为包被工作浓度,1:3 000为酶标工作浓度时,能较高特异性检测结核杆菌培养上清分泌的CFP10-ESAT-6抗原.     

Potential role of G-protein-coupled receptor 30 (GPR30) in estradiol-17beta-stimulated IGF-I mRNA expression in bovine satellite cell cultures

Androgenic and estrogenic steroids enhance muscle growth in animals and humans. Estradiol-17beta (E2) and trenbolone acetate (TBA) (a synthetic testosterone analog) increased IGF-I mRNA expression in bovine muscle satellite cell (BSC) cultures. The goal of this study was to evaluate the mechanisms responsible for this increase by evaluating the effects of ICI 182 780 (an E2 receptor antagonist), flutamide (an androgen receptor inhibitor), G1 (a GPR30 agonist), and BSA-conjugated E2 on E2 and/or TBA-stimulated IGF-I mRNA expression in BSC cultures. Flutamide completely suppressed TBA-stimulated IGF-I mRNA expression in BSC cultures. ICI 182 780 did not suppress E2-stimulated IGF-I mRNA expression and 100nM ICI 182 780 enhanced (93%, p<0.05) IGF-I mRNA levels in BSC cultures. G1 (100nM) stimulated IGF-I mRNA expression (100%, p<0.05) but had no effect on proliferation in BSC cultures. E2-BSA, which cannot cross the cell membrane, stimulated IGF-I mRNA expression (approximately 100%, p<0.05) in BSC but even at extremely high concentrations had no effect on proliferation. In summary, our data indicate the E2-stimulation of proliferation and E2-stimulation of IGF-I mRNA expression in BSC cultures occur via different mechanisms. Our previous results showing that ICI 182 780 inhibited BSC proliferation and results of the current study showing lack of response to E2-BSA or G1 suggest that E2-stimulated proliferation in BSC cultures is mediated through classical estrogen receptors. Stimulation by ICI 182 780, G1 and E2-BSA suggests the E2-stimulated IGF-I mRNA expression in BSC cultures is mediated through the GPR30 receptor.     

猪生殖与呼吸综合征病毒多基因重组真核表达质粒的构建及诱导的小鼠体液免疫

根据猪生殖与呼吸综合征病毒已知序列设计了3对引物,采用RT—PCR方法分别扩增ORF3、ORF5和ORF6基因,并将其依次与真核表达质粒pcDNA4．0定向连接,构建了重组质粒pcDNA4．0-ORF6-ORF3-ORF5,经提纯后,给试验组小鼠后肢胫前肌注射,每只100big,2周后再注射1次。首免后每隔7d采血1次,用间接ELISA检测血清抗体水平并测定其中和抗体水平。结果显示,所构建的重组质粒能够诱导小鼠产生很高的ELISA抗体和中和抗体水平。