Effect of menhaden fish oil supplementation and lipopolysaccharide exposure on nursery pigs. II. Effects on the immune axis when fed simple or complex diets containing no spray-dried plasma

A trial using 64 weanling pigs (TR4×PIC C22) was conducted to determine the effects of menhaden fish oil supplementation and diet complexity on performance and immune response of nursery pigs. Pigs (17 days and 6.27±1.16 kg) were weaned into a segregated early wean facility and given free access to a complex diet for 7 days post-weaning. At day 0 (day 7 post-weaning), pigs were blocked by weight and allotted to 64 pens. Treatments (Trt) were arranged as a 2×2×2 factorial arrangement. Main effects included diet (complex versus simple), oil (menhaden fish (MFO) versus corn (CO)), and immunogen (saline versus lipopolysaccharide (LPS)). Experimental diets contained 6% oil (6% CO or 5% MFO+1% CO) and were fed for 14 days. On day 12, i.v. injections of either LPS (150 μg/kg) or saline were given, followed by blood collection at 30 min intervals for 6 h. After the immune challenge (day 14), pigs were placed onto a common corn-soybean meal fortified diet and growth performance was evaluated until termination of the study (day 28). Pigs were weighed and feed intakes recorded at 7, 14, and 28 days. Prior to immune challenge (day 12), there were differences in BW for pigs fed complex versus simple diets (P<0.01; 13.1 and 12.1 kg, respectively) and pigs fed CO versus MFO diets (P<0.05; 12.9 and 12.3 kg, respectively). During the challenge period, for pigs treated with LPS there was a Time×Immunogen×Oil effect (P<0.001) for serum cortisol with MFO fed pigs having lower serum cortisol as compared to CO fed pigs. Also, during the challenge period, for pigs treated with LPS there was a Time×Diet×Immunogen×Oil effect (P<0.001) for serum tumor necrosis factor- (TNF-) with pigs fed complex diets supplemented with CO having higher serum TNF- as compared with pigs fed complex diets supplemented with MFO. At days 14 and 28, LPS-treated pigs had lower BW than saline injected controls (P<0.001 and 0.01, respectively). In addition, pigs fed simplified diets continued to have lower BW after challenge compared to pigs fed a complex diet. Interestingly, there were no differences (P>0.10) in BW after challenge in pigs fed MFO. This study suggests that MFO supplementation alters the immune response during LPS challenge and that simplified diets may compromise nursery performance.     

Tissue-specific antioxidative and neurotoxic responses to diazinon in Oreochromis niloticus

The purpose of this study was to evaluate oxidative stress and neurotoxic potential of organophosphorus (OP) insecticide diazinon in the sentinel freshwater fish, Oreochromis niloticus. Antioxidant and acetylcholinesterase (AChE) enzyme activities and malondialdehyde (MDA) and protein levels were measured spectrophotometrically in gill, kidney, alimentary tract, and muscle tissues of fish treated with sub-lethal diazinon concentrations for 1, 7, 15, and 30 days. Dose-dependent inhibitions of AChE were observed in all the experimental fish. On the contrary of alimentary tract, MDA levels were elevated in kidney and muscle and gill was not affected. AChE and MDA levels intercorrelated in kidney and muscle tissues. Diazinon had increased superoxide dismutase (SOD) activities in all the tissues, while kidney was the most affected tissue. Tissue-specific alterations were observed on catalase (CAT) and glutathione peroxidase (GPx) activities; however, the activities were not changed in gill and muscle tissues for GPx and in gill, muscle, and kidney tissues for CAT. Protein levels decreased in kidney, muscle, and alimentary tract, while increased in gill and alimentary tract in 15 days. With respect to these results, diazinon has oxidative and neurotoxic potentials in O. niloticus. Observed changes with diazinon treatment were generally tissue-specific and dose-dependent.     

耦合水文情势及鱼类繁殖的江垭水库生态调度需求研究（生态调度专刊）

大型水库实施生态调度是修复河流水文情势、维持河流生态系统健康的重要手段。江垭水库位于溇水干流下游的张家界慈利县，是澧水流域具有重要防洪意义的大型水库。本研究通过环境DNA采样、IHA水文指标变动分析和文献资料总结等方法，开展了江垭坝下水文情势及鱼类繁殖需求的关系研究，提出了江垭水库生态调度需求及建议。2021年9月在溇水干支流综合调查到鱼类55种，与上世纪90年代相比，流水性种类在干流江段显著减少了51.35%；按照生态调度优先等级评估原则，确定江垭水库生态调度的主要目标物种为产漂流性卵的银鲴、银鮈、贝氏?和产粘沉性卵的鲤、鲫；江垭建坝前后下游水文情势变化较大，发生高度改变且对鱼类洄游产卵及鱼卵发育有重要作用的水文指标包括：年最大1日、3日平均流量指标，年出现高流量脉冲事件的次数和持续时间，以及连续日流量上涨率。以促进坝下不同产卵类型的鱼类繁殖为目标，江垭水库生态调度一方面需要恢复一定的高流量脉冲次数和历时，另一方面需要控制下游的水位日降幅，通过泄放合理的生态流量来维持下游鱼类的生物多样性。     

Protein quality of larval feed with increased concentration of hydrolysed protein: effects of heat treatment and leaching

Four heat coagulated early weaning diets with increasing concentrations of pepsin hydrolysed protein, were investigated with regard to the change in protein quality during feed production and exposure to leaching. Water-soluble N, trichloroacetic acid-soluble N and amino acid (AA) profiles were determined in finished diets and in diets leached for 6 min. In vitro diet digestibility was measured and related to increasing inclusion of hydrolysed protein and N leakage. Seventeen to 47% of soluble N in the feed ingredients was made insoluble by heat denaturation during feed production, but the concentration of peptides and free amino acids (FAA) were not influenced. All peptides/FAA and 70–80% of water-soluble protein were lost after exposure to leaching. Increased inclusion of hydrolysed protein increased the loss of crude protein (15–30%). All taurine and 30% of histidine was lost during leaching, no other major changes in AA profile were found. There was no difference in digestibility between diets exposed to leaching. However, leached diets showed reduced digestibility as compared to diets that had not been exposed to leaching. In conclusion micro-bound type diets as used in this study have a low efficiency in delivering soluble N to fish larvae and should be carefully considered for this purpose.     

Essential Fatty Acid Supplementation In The Treatment Of Canine Atopy: A Preliminary Study

Abstract— In an open, uncontrolled study, 10 dogs with atopy were given evening primrose oil (EPO) (5ml/10kg/day) over a period of 9 weeks. During the first three weeks pruritus, scaling and oedema worsened but erythema and coat condition tended to improve. Subsequently all these parameters tended to improve. The effect on oedema was significant (p < 0.05). Eight of these dogs and two others were then given a 4:1 mixture of EPO and marine fish oil for 9 weeks. Although further reductions in scale and improvements in coat condition were marked in some animals, no significant changes occurred. Alterations in the plasma phospholipid essential fatty acid levels reflected the compositions of the two supplements. These findings suggest that EPO can ameliorate of atopy in dogs. This mirrors observations obtained in studies of human atopic eczema. Résumé— Dans une étude ouverte, sans lot témoin, 10 chiens souffrant d'atopie ont reçu de l'huile d'onagre (5 ml/10 kg/jour) pendant une période de 9 semaines. Pendant les 3 premières semaines, le prurit, le squamosis et l'oedème se sont aggravés mais, l'érythème et l'aspect du poil tendaient à s'améliorer. Enfin tous ces parametres tendaient à s'améliorer. L'effet sur l'érythème a été significatif (p < 0.05). Huit de ces chiens et deux autres ont alors reçu un mélange (4:1) d'huile d'onagre et d'huile de poisson de mer pendant 9 semaines. Bien qu'ultérieurement la diminution du squamosis et l'amélioration de l'état de la fourrure aient été manifestes chez certains animaux, il n'y eut pas de changement significatif. Les modifications des taux d'acides gras essentiels des phospholipides plasmatiques ont reflété la composition des deux supplémentations. Ces observations suggèrent que l'huile d'onagre peut apporter une amélioration de l'atopie canine. Cela reflète certains résultats observés lors d'études sur l'eczéma atopique de l'homme. Zusammenfassung— In einer offenen, nicht kontrollierten Untersuchung wurde 10 Hunden mit Atopie Nachtkerzenöl (EPO) in einer Dosierung von 5 ml/10 kg/Tag über einen Zeitraum von 9 Wochen verabreicht. Während der ersten 3 Wochen verschlimmerten sich Pruritus, Schuppenbildung und Ödeme, während Erytheme und Fellzustand eher eine Tendenz zur Besserung zeigten. Danach besserten sich alle diese Parameter. Die Wirkung auf Erytheme war signifikant (p < 0.05). Acht dieser Hunde und zwei andere erhielten 9 Wochen lang Nachtkerzenöl und Seefischöl in einer Mischung von 4:1 im Anschlufl an diese Untersuchung. Obwohl eine weitere Verminderung der Schuppenbildung und eine Vorbesserung der Fellqualität bei einigen Tieren festgestellt wurden, waren diese Veränderungen nicht signifikant. Die Veränderungen der essentiellen phospholipiden Fettsäuren im Plasma spiegeln die Zusammensetzung der beiden verabreichten Öle wieder. Diese Befunde weisen darauf hin, daß die Atopie des Hundes durch EPO gebessert werden kann. Dies entspricht auch Beobachtungen bei Untersuchungen des atopischen Exzems des Menschen.     

膨化大豆替代鱼粉对乌苏里貉毛皮质量的影响

选取150只健康乌苏里幼貉(Nyctereutes ussurienusis Matschie),随机分成1个对照组和4个试验组.各试验组按25%、50%、75%、100%比例用膨化大豆替代鱼粉饲料进行饲养试验,通过对针、绒毛的长度、粗细和皮板长度等反映毛皮质量各项指标的测定与分析得出:彭化大豆代替鱼粉能满足乌苏里貉冬毛生长的需要,不影响乌苏里貉毛皮质量.     

玉米芯水解液发酵生产木糖醇的研究

假丝酵母（Candida sp.)菌株经驯化后显著地提高了对水解液中发酵抑制物质的耐受力，从而增加了木糖利用率和木糖醇得率，玉米芯水解液经过石灰中和后，在30℃下采用台化后的假丝酵母菌株直接发酵生产木糖醇，对发酵条件进行了优化，优化结果为：接种量10%（体积比），种子龄17h，氮源组成：1.5g/L的酵母浸膏，2.5g/L的胰蛋白胨，250mL三角瓶装液120mL，初始pH值5.5。在此条件下，木糖的利用率达79.8%，木糖醇得率达62.4%^，该方法大大降低了预处理的成本，显示了良好的应用前景。     

Effects of food enriched with egg yolk hydrolysate (bone peptide) on bone metabolism in orchidectomized dogs

We examined the effects of chicken egg hydrolysate (also known as “bone peptide” or BP) on bone metabolism in 5- to 8-month-old orchidectomized dogs. The bone formation marker serum bone alkaline phosphatase (BAP) and the bone resorption marker urine deoxypyridinoline (DPD) were used as indicators to measure changes in bone metabolism. The following results were observed that Serum BAP was higher in dogs fed BP-enriched food throughout the clinical investigation. Serum BAP was statistically significantly higher in dogs fed BP-enriched food than in dogs fed non-BP-enriched food at 2 months after orchidectomy. This suggests that BP promoted bone formation immediately after orchidectomy.     

波浪作用下半潜式养殖网箱水动力特性

半潜式深远海养殖网箱在波浪作用下会发生变形与运动。为保证网箱结构的稳定性,需对其水动力特性进行分析。本研究基于有限元法建立了波浪作用下一种半潜式网箱的数值模型,通过仿真计算求解网箱的锚绳受力与运动情况。首先,将计算机模拟值与物理水槽试验值进行比较,验证数值模型的准确性。然后,分别研究了半潜式网箱在3种压载状态下的动力响应情况,分析比较了不同波浪条件下网箱锚绳张力、垂荡、纵荡和纵摇的计算结果。结果显示,计算值与试验值基本吻合,二者的相对误差在5%左右。当波高一定时,网箱迎浪侧和背浪侧锚绳受力与波浪周期改变无明显关联;当周期一定时,两侧锚绳受力均随波高的增加而增大。网箱的垂荡、纵荡及纵摇值均与波高呈正相关,随着半潜式网箱吃水的增加,网箱的垂荡、纵荡及纵摇值基本呈减小趋势。网箱在3种压载状态下最大垂荡值和纵荡值分别为12.67 m和10.59 m,网箱在空载状态下的最大纵摇值≤15°,表明半潜式网箱结构具有较好的稳定性。研究结果可为我国深远海养殖网箱设计提供理论参考。