Influence of pond fertilization and feeding rate on growth performance, economic returns and water quality in a small-scale cage-cum-pond integrated system for production of Nile tilapia (Oreochromis niloticus L.)

The effects of pond fertilization and feeding rate on growth, economic returns and water quality were investigated to develop a low‐cost cage‐cum‐pond integrated system for production of Oreochromis niloticus (L.). Hand‐sexed male fingerlings averaging 19±0.39 and 32±0.69 g were stocked in cages and open ponds at 150 fish cage^?1 and 2 fish m^?2 respectively. Fish were cultured for 114 days in five triplicate treatments. Cages were installed into ponds and caged fish were fed a 24% protein diet at 3% (T1) and 6% (T2) body weight day^?1 (BWD) without pond fertilization, and 6% BWD with pond fertilization (T3). The open water in the fourth treatment (T4) was not stocked but contained caged fish, which were fed 6% BWD for the first 57 days followed by 3% BWD for the remaining period. Ponds in the control (T5) had no cages and were neither fertilized nor open‐pond fish fed. Feeding rate and pond fertilization significantly (P<0.05) affected fish growth, profitability and water quality among treatments. Fish growth, feed utilization, fish yield, water quality and profits were significantly (P<0.05) better in T3 than the other treatments. It was concluded that fish production and economic returns were optimized at 6% BWD in fertilized ponds.     

黄河班多段鱼类多样性初步研究及保护对策

黄河班多段位于青海省兴海县境内,是黄河上游主要鱼类分布区之一.2011年4-11月对黄河班多段鱼类资源进行了调查.设采样点4个,分别在鱼类生长繁殖的重要时期使用不同网具采捕4次,并结合走访调查及鱼类样本的实验室鉴定与分析,重点研究鱼类种类、鱼类多样性、丰富度及均匀度,并提出了保护对策.结果表明,本次调查共采集鱼类10种、138尾,均为土著鱼类,种类占黄河上游青海段鱼类近1/2,班多段鱼类多样性相对黄河上游青海段其它水域较高,鱼类组成与黄河上游青海段其它水域相似,种类相对比较丰富,涉及多种保护鱼类,但每种鱼的个体均匀度较差,抗外界扰动的能力差.     

波浪作用下半潜式养殖网箱水动力特性

半潜式深远海养殖网箱在波浪作用下会发生变形与运动。为保证网箱结构的稳定性,需对其水动力特性进行分析。本研究基于有限元法建立了波浪作用下一种半潜式网箱的数值模型,通过仿真计算求解网箱的锚绳受力与运动情况。首先,将计算机模拟值与物理水槽试验值进行比较,验证数值模型的准确性。然后,分别研究了半潜式网箱在3种压载状态下的动力响应情况,分析比较了不同波浪条件下网箱锚绳张力、垂荡、纵荡和纵摇的计算结果。结果显示,计算值与试验值基本吻合,二者的相对误差在5%左右。当波高一定时,网箱迎浪侧和背浪侧锚绳受力与波浪周期改变无明显关联;当周期一定时,两侧锚绳受力均随波高的增加而增大。网箱的垂荡、纵荡及纵摇值均与波高呈正相关,随着半潜式网箱吃水的增加,网箱的垂荡、纵荡及纵摇值基本呈减小趋势。网箱在3种压载状态下最大垂荡值和纵荡值分别为12.67 m和10.59 m,网箱在空载状态下的最大纵摇值≤15°,表明半潜式网箱结构具有较好的稳定性。研究结果可为我国深远海养殖网箱设计提供理论参考。     

烟威近岸海域鱼类早期资源群落结构及适宜产卵生境

烟威近岸海域是历史上重要的鲐(Scomber japonicus)产卵场之一,或囿于对其“过路渔场”的认知,近年来对该海域鱼类产卵场的研究相对较少。为了解烟威近岸海域鱼类产卵场现状,于2020年4—9月对该海域开展逐月产卵场调查,基于鱼卵、仔稚鱼及环境数据,运用空间插值、聚类分析、非度量多维标度排序、相似性分析和冗余分析(RDA)等方法对该海域鱼类早期资源时空分布、群落结构月度更替及主要种类适宜产卵生境进行了综合分析。结果显示,2020年4—9月于烟威近岸海域采集到鱼类早期资源种类52种,包括33种鱼类的337 038粒鱼卵和28种鱼类的2122尾仔稚鱼;5—6月为主要产卵期,共有21种鱼卵出现,鱼卵数量占全年鱼卵总数的98.32%,主要产卵场位于烟台套子湾至威海鸡鸣岛北部海域,主要产卵种类为鳀(Engraulis japonicus)、鲐、蓝点马鲛(Scomberomorus niphonius)、高眼鲽(Cleisthenes herzensteini)、黄条 (Seriola lalandi)、绯 (Callionymus beniteguri)、少鳞 (Sillago japonica)、黑鲷(Acanthopagrus schlegelii)、短吻红舌鳎(Cynoglossus joyneri)、长蛇鲻(Saurida elongata)等;烟威近岸海域鱼卵与仔稚鱼群落结构年内变化明显,除产卵末期(8—9月)外,各月间种类更替率不低于50%,鱼卵与仔稚鱼群落月间平均相异性不低于73%;海表温度(SST)、海底温度(SBT)、海表盐度(SSS)和深度(DEP)是显著影响4—9月主要鱼种产卵选择的环境因子。研究表明,烟威近岸海域为黄渤海规模较大的鱼类产卵场之一,需在鱼类早期生活史研究与产卵场养护策略制定时得到足够重视。     

Relationship between zooplankton abundance and the early marine life history of juvenile chum salmon <Emphasis Type="Italic">Oncorhynchus keta</Emphasis> in eastern Hokkaido,Japan

Interannual variations in abundance, timing of outmigration from rivers, growth rate and condition of juvenile chum salmon (Oncorhynchus keta) were studied in the Nemuro Strait (eastern Hokkaido, Japan) during 1999–2002 to establish a possible relationship to zooplankton abundance. The otolith microstructure of juveniles was examined each year in late June to determine their time and size at sea entry (i.e., outmigration), and to estimate the early marine growth rates. Salmon outmigration peaked in mid- or late May, which coincided, in three of the four study years, with the peak release of juveniles into rivers within the study area. Abundance, growth rate and condition of fish were higher in 2001, when—compared to other years—smaller fish experienced higher growth rates, coinciding with greater zooplankton abundance for that year. Our results suggest that high zooplankton abundance positively influenced juvenile chum salmon growth and the condition of the fish during their early marine life despite their small size at sea entry.     

Characteristics of a self-assembled fibrillar gel prepared from red stingray collagen

A translucent collagen gel was formed from a transparent acidic solution of red stingray collagen by adjusting to physiological ionic strength and pH in phosphate buffer and then incubating at 25–37°C. During fibril formation from red stingray collagen, the turbidity increased when the NaCl concentration was increased at constant pH and the rate of fibril formation was accelerated by higher pH or lower NaCl concentration. The T _m of red stingray collagen fibrillar gel was estimated as 44.3 ± 3.5°C, which was higher than that of the collagen solution, 33.2°C. In addition, red stingray collagen gel maintained its shape without melting and was suitable for culture of mouse stromal cells at 37°C.     

The risk of parasite transfer to juvenile fishes by live copepod food with the example Triaenophorus crassus and Triaenophorus nodulosus

Natural zooplankton is a potential food resource for juvenile fish in fish farms as it is a good source of fats, carbohydrates, and protein. However, it is also a potential source of parasites and pathogens. The present study was conducted (1) to estimate the risk of parasite transfer by live copepod food under intensive farming conditions using the parasites Triaenophorus crassus and Triaenophorus nodulosus as example and (2) to look for strategies to avoid parasite infestation of juvenile fish.An easy and routinely applicable aniline blue staining method was developed to check the infestation levels of copepods with procercoids during the on-growing season of juvenile fish. From the copepod species occurring in zooplankton Cyclops spp. was infested frequently (9.4 ± 12.0%, maximum 38.7%), Diaptomus spp. infrequently (1.5 ± 1.6%, maximum 3.2%), Daphnia spp. never. Juvenile grayling, Thymallus thymallus, and corgonids, Coregonus sp., which had been fed with natural zooplankton revealed infestations rates with T. crassus of circa 5%, with T. nodulosus of circa 10%. As the occurrence of procercoids in the zooplankton was temporary limited, Triaenophorus infestation can be avoided by using artificial food instead of live copepods during the risky season.To prevent parasite infestation of juvenile fish methods were investigated to eradicate procercoids from copepods by chemical treatment (sodium chloride, hydrogen peroxide, citric acid treatment) and by freeze-thawing methods. Chemical methods failed to remove procercoids. In fish, which had been fed with frozen thawed zooplankton, no Triaenophorus spp. infestation was observed. Their survival rates were similar as in the control fed with live zooplankton, however the weight of the fish was significantly lower.     

2020年湘江长沙段仔稚鱼资源现状及其对水文环境的响应

研究仔稚鱼资源时空分布及其与水文环境的关系,为湘江仔稚鱼资源监测及长江十年禁渔效果评估提供本底数据及理论参考。2020年5-6月,在湘江下游长沙香炉洲江段进行为期57 d的仔稚鱼资源调查,以弶网采集鱼卵及仔稚鱼,每周采样2 d,每次采样时长15min。结果显示(：1)采集点共出现苗汛7次,第一次为5月22日,最后一次为6月29日,7次鱼汛平均水温为(26.5±0.57)℃,网口平均流速为(0.237±0.095)m/s;(2)共采集仔稚鱼547 831尾,隶属5目8科41种,以产沉性卵鱼类(23种)和广适性鱼类(24种)为主,分别占56.1%、58.5%,鲤科鱼类31种、占比75.6%,优势种9种：子陵吻虾虎鱼(Rhinogobius giurinus)、飘鱼(Pseudolaubuca sinensis)、大鳍鱊(Acheilognathus macropterus)、银鲴(Xenocypris argentea)、鲤(Cyprinus carpio)、?(Hemiculter leucisculus)、黄尾鲴(Xenocypris davidi)、鲫(Carassius aurat...     

鱼类血液基因组DNA提取方法优化

The genomic DNA extracted from frozen whole blood of rainbow trout with methods of chloroform-potassium acetate, rapid genomic DNA extraction （RGDE） , improved RGDE, traditional phenol-chloroform and DNA reagent kit respectively, and detected by agarose gel electrophoresis. The results showed that mothode of improved RGDE can obtain better genomie DNA of fish. Then, the effect of extracting with improved RGDE methods were compared by ultraviolet spectrophotometric method with other mothods such as traditional phenol-chloroform from blood or muscle and DNA reagent kit from blood. The results showed that the average concentration of DNA extracted with method improved RGDE was 1 050 ng/μL, and was very significant higher than that by DNA kit or by phenol- chloroform, and the average output of DNA was 1 575μg/mL, and the rate of A260/A280 was between 1.65 - 1.97. It only spent 2 hours to extract DNA with the method of improved RGDE, and there were many obvious merits such as quick speed, low cost, no pollution and no using specific installations and so on, and to amplify by random primers, the rusuhs was stable,and the electrophoresis banding was clear.     

三峡库区万州段投饵网箱养鱼对底泥水化因子的影响

2005年6月至2006年4月对长江三峡库区投饵网箱养殖区上游、网箱养殖区以及网箱养殖区下游底泥中有机物耗氧量、TN、NO3-N、TP、硫化物等指标进行了监测。结果显示,网箱养殖区及网箱下游底泥有机物耗氧量和总磷含量分别高出对照区5.4～6.5 mg/g和0.03～0.08 g/kg,网箱养殖区及网箱下游底泥总氮含量及硝态氮含量分别高出对照区40～80 mg/kg和0.8～1.2 mg/kg,网箱养殖区及网箱下游底层水的硫化物含量平均值分别高出对照区1.3 mg/L和0.11 mg/L。