坡向和海拔对乌孙山草地植物群落特征及物种多样性的影响

【目的】研究不同坡向、不同海拔梯度的草地植物群落特征差异,为合理利用草地资源提供科学依据。【方法】以位于西天山支脉乌孙山南麓的昭苏县和乌孙山北麓的察布查尔县天然草地为对象,采用典型的样方调查法,分析不同坡向(南坡、北坡)和海拔梯度(1 600~3 000 m)的草地植物群落物种组成,群落数量特征、物种多样性,研究草地植物群落物种多样性在不同海拔和坡向上的分布规律。【结果】同一海拔梯度上,南坡植物物种数多于北坡;在不同坡向上,基本上南坡各海拔草地植物群落高度大于北坡,而密度和盖度小于北坡。南坡各多样性指数沿着海拔高度变化呈现出双峰格局;北坡Simpson指数、Shannon-Wiener指数和Pielou指数沿着海拔高度变化均呈现出波浪型变化趋势,Patrick指数则呈现出双峰格局。【结论】乌孙山植物群落特征及物种多样性的变化是由海拔和坡向为主的多种环境因子综合作用的结果。     

一种基于解耦旋转锚框匹配策略的谷粒检测方法

【目的】基于计算机视觉技术建立水稻考种中谷粒计数与粒径的检测方法,节省考种人力成本,提升效率,有利于数字化育种体系建立。【方法】通过对比基于 YOLOv5l 骨架结构的水平框目标检测策略与基于 ResNet101 和 DarkNet53 骨架结构的旋转框检测策略,同时比较旋转框检测策略预测的水稻谷粒长宽比值与实际测量值之间的差异,验证该方法的可行性。【结果】旋转框的目标检测模型计算得到的水稻谷粒长宽比与实测值无显著差异,两者的均方根误差为 0.91~2.29;而水平框的目标检测模型计算的长宽比显著小于实测值,且均方根误差值（9.38~9.45）比旋转框的目标检测模型更大。基于水平框的目标检测策略与基于旋转框的检测策略在谷粒计数的精度几乎相当,但水平框检测模型无法实现水稻谷粒长宽比的准确计算,而使用旋转框的目标检测策略能够较为准确计算谷粒长宽比。【结论】基于解耦旋转锚框匹配策略可对水稻谷粒进行准确计数,相比传统水平框的目标检测策略,可降低检测中的背景噪声和密集堆积物体的漏检情况,同时快速准确计算水稻谷粒长宽比。该方法可以进一步应用到水稻品种识别中的谷粒长短和大小的计算,以及种子质量检测及品种鉴定等数字化育种场景中。     

波浪作用下半潜式养殖网箱水动力特性

半潜式深远海养殖网箱在波浪作用下会发生变形与运动。为保证网箱结构的稳定性,需对其水动力特性进行分析。本研究基于有限元法建立了波浪作用下一种半潜式网箱的数值模型,通过仿真计算求解网箱的锚绳受力与运动情况。首先,将计算机模拟值与物理水槽试验值进行比较,验证数值模型的准确性。然后,分别研究了半潜式网箱在3种压载状态下的动力响应情况,分析比较了不同波浪条件下网箱锚绳张力、垂荡、纵荡和纵摇的计算结果。结果显示,计算值与试验值基本吻合,二者的相对误差在5%左右。当波高一定时,网箱迎浪侧和背浪侧锚绳受力与波浪周期改变无明显关联;当周期一定时,两侧锚绳受力均随波高的增加而增大。网箱的垂荡、纵荡及纵摇值均与波高呈正相关,随着半潜式网箱吃水的增加,网箱的垂荡、纵荡及纵摇值基本呈减小趋势。网箱在3种压载状态下最大垂荡值和纵荡值分别为12.67 m和10.59 m,网箱在空载状态下的最大纵摇值≤15°,表明半潜式网箱结构具有较好的稳定性。研究结果可为我国深远海养殖网箱设计提供理论参考。     

烟威近岸海域鱼类早期资源群落结构及适宜产卵生境

烟威近岸海域是历史上重要的鲐(Scomber japonicus)产卵场之一,或囿于对其“过路渔场”的认知,近年来对该海域鱼类产卵场的研究相对较少。为了解烟威近岸海域鱼类产卵场现状,于2020年4—9月对该海域开展逐月产卵场调查,基于鱼卵、仔稚鱼及环境数据,运用空间插值、聚类分析、非度量多维标度排序、相似性分析和冗余分析(RDA)等方法对该海域鱼类早期资源时空分布、群落结构月度更替及主要种类适宜产卵生境进行了综合分析。结果显示,2020年4—9月于烟威近岸海域采集到鱼类早期资源种类52种,包括33种鱼类的337 038粒鱼卵和28种鱼类的2122尾仔稚鱼;5—6月为主要产卵期,共有21种鱼卵出现,鱼卵数量占全年鱼卵总数的98.32%,主要产卵场位于烟台套子湾至威海鸡鸣岛北部海域,主要产卵种类为鳀(Engraulis japonicus)、鲐、蓝点马鲛(Scomberomorus niphonius)、高眼鲽(Cleisthenes herzensteini)、黄条 (Seriola lalandi)、绯 (Callionymus beniteguri)、少鳞 (Sillago japonica)、黑鲷(Acanthopagrus schlegelii)、短吻红舌鳎(Cynoglossus joyneri)、长蛇鲻(Saurida elongata)等;烟威近岸海域鱼卵与仔稚鱼群落结构年内变化明显,除产卵末期(8—9月)外,各月间种类更替率不低于50%,鱼卵与仔稚鱼群落月间平均相异性不低于73%;海表温度(SST)、海底温度(SBT)、海表盐度(SSS)和深度(DEP)是显著影响4—9月主要鱼种产卵选择的环境因子。研究表明,烟威近岸海域为黄渤海规模较大的鱼类产卵场之一,需在鱼类早期生活史研究与产卵场养护策略制定时得到足够重视。     

Metabolic effects of changes in the dietary protein: carbohydrate ratio in eel (Angilla anguilla) and trout (Oncorhynchus mykiss)

The metabolic consequences of the isoenergetic replacement of dietaryprotein by carbohydrates (CHO) were compared in studies of European eel andrainbow trout. Diets with 45/20, 37/30, 29/40 and 21/50 percentprotein/carbohydrate were assessed during a 12-week experiment. The CHO sourcewas pre-gelatinized corn starch. Fish of initial average weight 45gwere fed to satiation twice daily. Weight-gain and feed-efficiency data weregenerally better in trout than in eel, presumably because trout digestibilitycoefficients for protein and energy were higher than in eel. In both species,regardless of physiological differences in digestion and absorption, feedintakeand nutrient digestibility were lower when dietary CHO was higher, but theinter-species differences decreased with increasing dietary CHO.Pyruvate kinase activity (PK) in trout was not affected by dietary CHO content,while blood-glucose and liver-glycogen levels significantly rose withincreasingdietary CHO. In contrast, blood-glucose levels appeared to be regulated ineels,perhaps by an accelerated glycolysis rate, revealed by changes in PK.Gluconeogenic activity was inhibited in trout fed a diet containing20–30%CHO, while in eels this activity was not inhibited by dietary replacement ofprotein by carbohydrate. Liver glucose 6-phosphate dehydrogenase activity(G6PDH) was inhibited in trout fed increasing dietary CHO, whereas in eelsG6PDHoperated at a high rate regardless of dietary composition. These results mayindicate that eels have a better capacity for metabolising high-CHO/low-proteindiets than do trout.     

The Ionic and Osmotic Factors Controlling Motility of Fish Spermatozoa

This review presents actual knowledge about energetic, ionic, osmotic and gaseous control of fish sperm motility and its duration. Right after they are activated, fish spermatozoa of most species swim for a short period of time, in the range of one to several minutes. What determines the activation process? Is it due to the new ionic, gaseous and/or osmotic environment? Why is the duration of motility so short? Is it resulting from a fast exhaustion of energy stores (ATP, ADP, AMP, PCr) combined with the above-mentioned ionic/osmotic stress leading to morphological alterations? The motility criteria (flagellar beat frequency, head displacement velocity, flagellar waves morphology, etc.) used to characterize fish sperm movement and sperm flagella will be described. Most parameters change very rapidly during the brief motility period of fish sperm. Then will be considered the main environmental factors, ionic and/or osmotic signals, responsible of the activation of fish sperm motility. Then the metabolic compounds involved in cell energetics will be considered as their concentrations also rapidly change during the motility phase. An additional feature will then be discussed concerning the mechanisms by which fish sperm cell can be revived for a second motility round at the end of the first motility period. A model is proposed to explain how external osmolarity can control internal ionic composition, the latter being the key factor controlling flagellar activity.     

Capacity for digestive hydrolysis and amino acid absorption in Atlantic salmon (Salmo salar) fed diets with soybean meal or inulin with or without addition of antibiotics

This experiment was done to study the effects of dietary soybean meal (SBM) and inulin (a prebiotic) on the capacity for digestive hydrolysis and amino acid absorption by Atlantic salmon, and how a dietary supplement of the broad-spectrum antibiotic oxytetracycline (OTC) modulated these responses. A control diet (FM) was made from fish meal, fish oil and extruded wheat. Two similar diets were made with 250 g soybean meal (SBM) or 75 g inulin kg^− 1. Each diet was made with or without a supplement of 3 g OTC kg^− 1. All six diets contained yttrium oxide for estimation of apparent nutrient absorption. Each diet was fed to two groups of 172 g salmon kept in 1 m² tanks with 9 °C saltwater for 3 weeks. Intestinal organs were then sampled and weighed. Gastrointestinal tracts (GIT) were sectioned for analyses of brush border alkaline phosphatase (ALP) and leucine aminopeptidase (LAP) activities. Tissue from the distal intestine (DI) was also fixed for histological examination. Digesta from the different sections were freeze dried for estimation of trypsin and amylase activities, and of apparent absorption of amino acids (AA), nitrogen (N), and sulphur (S). About 85% of the trypsin activity, 70% of the amylase activity, 85% of the ALP activity, and 82% of the LAP activity were found in the proximal (PI) and mid (MI) intestine of fish with functional DI, and the absorption of AA, N, and S was quantitatively completed in the MI. Dietary OTC resulted in lower relative liver weight, but apart from increased ALP and LAP activities in DI when feeding OTC in combination with inulin, OTC did not modify the responses to dietary SBM or inulin. Dietary SBM resulted in lower relative liver weight, and induced pathomorphological changes in the DI mucosa, thus lower the ALP and LAP activities in the DI. SBM also stimulated absorption of AA, N, and S in the PI, but at the same time increased the activities of trypsin and amylase in the DI, indicating reduced re-absorption and increased faecal losses of these endogenous enzymes. Dietary inulin did not damage the DI, and stimulated intestinal growth and higher relative mass of the GIT. Inulin without OTC did not affect the hydrolytic and absorptive capacity of the salmon GIT.     

Molasses as C source for heterotrophic bacteria production on solid fish waste

The drumfilter effluent from a recirculation aquaculture system (RAS) can be used as substrate for heterotrophic bacteria production. These bacteria can be reused as aquatic feed. In RAS drumfilter effluents are organic carbon deficient for bacteria production. This is due to nitrogen accumulation in the system water. In the present experiment, a bacteria growth reactor (3.5l) was connected to the drumfilter (filter mesh size 60 μm) outlet of a recirculation system. To counteract carbon deficiency, different supplementation levels of molasses (organic carbon) were tested (carbon fluxes of 0.0, 3.2, 5.8, 7.8, 9.7 gC molasses/l/d; C:N ratios: 3.4, 6.4, 9.4, 13.0 and 16.5). The hydraulic retention time in the bacteria reactor was 6 h. For the maximum flux, the VSS and crude protein productions were about 168 gVSS and 95 g crude protein per kg feed. The maximum conversion of nitrate and ortho-phosphate was 24 g NO₃₋N and 4 gP/kg feed. This equals a conversion of 90% of the inorganic nitrogenous waste and 98% of the ortho-phosphate-P. Furthermore the maximum substrate removal rate and the K_s for molasses were determined (1.62 gC/l/h and 0.097 gC/l respectively). The maximum specific removal rate was 0.31 gC/gVSS/h and the related half saturation constant was 0.008 gC/l. The observed growth rate reached a maximum for C fluxes higher than 8 g/l/d. The present integration of heterotrophic bacteria production in RAS represents, therefore, an innovative option to reduce waste discharge by conversion. If the bacteria are reused as feed, system's ecological sustainability increases.     

鲤鱼单极虫病组织病理研究

本文报道了1989年以来,造成王快水库等网箱养鲤大批死亡的单极虫病,病鱼出现烂鳃、肛门红肿、腹部膨大,腹腔内有大量粘液等症状;显微观察的结果为:各组织器官发生病变,肠上皮细胞严重坏死,平滑肌纤维发生玻璃样变及解体,鳃丝充血,鳃小片融合,肝细胞核溶解、坏死。     

江河船体式网箱养鱼的评述

作者认为，江河船体式网箱养鱼作为一种新型的养殖方式，应对饲养对象的选择，流量控制，汛期的环境变化（水位，泥沙含量，固体污染物侵袭），以及船体的材料结构等进行深入研究，取得必要的基础资料，以促进江河船体式网箱养鱼稳步健康地发展。