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YAN LIU CHAOXIAN ZHANG SHOUHUI WEI HAILAN CUI HONGJUAN HUANG 《Weed Biology and Management》2011,11(3):160-166
The allelopathic effects of the exotic invasive weed, Johnsongrass, on Lactuca sativa, a native plant in China, were evaluated and the phytotoxins were investigated under laboratory conditions. The crude extracts (chloroform and ethyl acetate fractions) that were obtained from the ethanol extract of the subterranean parts of Johnsongrass inhibited the germination speed and growth of the roots and shoots of the test plant. The four compounds, ethyl p‐hydroxybenzoate, diosmetin, apigenin, and luteolin, were isolated from the extract of the subterranean parts of Johnsongrass first, along with three other compounds (reported previously), p‐hydroxybenzaldehyde, p‐hydroxybenzoic acid, and dhurrin, and they all were evaluated on L. sativa. At the concentration of <0.5 mmol L?1, ethyl p‐hydroxybenzoate, dhurrin, and the mixture of the compounds delayed the germination speed of the seeds of L. sativa at 24 h of incubation, while apigenin delayed the germination speed of the seeds of L. sativa at 72 h of incubation. Ethyl p‐hydroxybenzoate demonstrated the strongest delaying effect among the phenols and a similar effect was found with apigenin among the flavonoids, wheras ethyl p‐hydroxybenzoate induced a drastic inhibition of the germination at 2 mmol L?1. In contrast, p‐hydroxybenzaldehyde and luteolin had no effect on the germination at any concentration at any stage. All the substances inhibited the shoot and root growth of L. sativa at 3 mmol L?1. Increasing the concentration increased the inhibition of the growth of L. sativa. The inhibitory activity of ethyl p‐hydroxybenzoate and p‐hydroxybenzaldehyde was greater than that of the other compounds. This result suggested that the isolated phytotoxins might contribute to the successful invasion by Johnsongrass. 相似文献
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A pot experiment was conducted to investigate the effect of growing soybean on N2O emission from soil. When soybean was growing in pots, the cumulative N2O emission during the growing season was 2.26 mg N pot−1, which was 5.9 times greater than that from the identical but unplanted pots (CK). However, the difference in N2O fluxes between the two treatments was not significant until the grain-filling stage. Of the total N2O emission, 94% took place during the period from grain-filling to ripening. Premature harvesting of the aerial parts of the plants at various growth stages substantially stimulated N2O emission from the soil. These results implied that the process of symbiotic N fixation per se does not stimulate N2O production or emission, but rather senescence and decomposition of the roots and nodules in the late growth stage. Therefore, additional N2O would be emitted from the soil after harvesting of soybean with roots, litter, and residues left in situ. 相似文献
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川中丘陵区不同利用方式的土壤养分特征研究 总被引:7,自引:0,他引:7
通过实地调查、土壤采样分析和数理统计结合的方法,对川中丘陵区5种土地利用方式(水田、旱地、菜地、林地和荒草地)的土壤养分状况进行对比分析,结果表明.土地利用方式对土壤有机质、全氮、全钾、全磷、碱解氮、速效钾和速效磷含量的影响达极显著(或显著)水平;在5种土地利用方式中.土壤有机质和全氮含量由高到低的顺序为:水田〉林地〉菜地〉旱地〉荒草地;土壤全钾和速效钾含量由高到低的顺序为:水田〉菜地〉旱地〉林地〉荒草地;土壤全磷含量由高到低的顺序为:菜地〉旱地〉林地〉水田〉荒草地。 相似文献
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碱性土壤基因组DNA的分离纯化和基因文库的构建 总被引:4,自引:0,他引:4
直接从广西南宁市凤凰纸业排污沟碱性土壤样品中抽提和分离纯化混合基因组DNA,所获得DNA的产量为每克土壤样品10~30pg。采用限制性内切酶EcoR1酶处理后,构建了以pGEM-3Zf( )为载体的DNA部分文库。文库的容量为23650个转化子。外源片段DNA平均大小为3.2kb。建库效率为每克环境样品获得6000个左右的含1~15kb外源随机插入片段的克隆。通过DNA序列测定和同源性比较,对从文库随机词取的16个转化子序列进行分析,发现13个外源插入片段包含序列尚未确定的DNA片段。 相似文献