秋花生新品系比较试验

用9个杂交花生品系与泉花7号（对照）进行比较试验,结果显示：12167的荚果产量比对照增产达极显著水平,抗病性、主要农艺及经济性状表现突出,可进一步试验观察.     

紫斑病对大豆室内发芽特性的影响

通过紫斑病对大豆室内发芽特性的影响研究,结果表明,大豆感染紫斑病后,种子的发芽势、发芽率、活力指数明显降低,不同感染级别种子的发芽势间存在明显差异,因此,大豆种子感染紫斑病后会因播种品质显著降低而不适于大田用种.     

红麻种质资源遗传多样性和分子鉴定技术研究I．红麻AFLP—银染技术和种质资源指纹图谱的构建

通过摸索和优化实验条件，建立了红麻的AFLP—银染实验体系，获得了清晰的红麻种质资源AFLP指纹图谱。通常不同品种的红麻种质资源在形态特征上差异很小，但AFLP—银染技术检测到不同来源和不同品种的在红麻种质资源中存在较大的遗传差异。这一技术对红麻种质资源的鉴定和遗传多样性的检测非常有效。     

Penetration of potato tuber lenticels by bacteria in relation to biological control of blackleg disease

Summary In a glasshouse experiment three potato cultivars were grown to maturity in wet or dry compost. The number of lenticels per tuber was greater with larger tubers but was unaffected by the cultivar or soil moisture regime. The frequency of lenticel penetration by a water soluble stain, safranin O, remained high as tubers developed to maturity in wet soil. In dry soil penetration declined markedly as the tubers matured. When tubers, harvested just before the onset of haulm senescence, were inoculated withErwinia carotovora ssp.atroseptica, the frequency of lenticel rotting was less than the rate of stain penetration, indicating that additional factors were involved in the bacterial infection process. Inoculating mature tubers with bacteria and incubating them in anaerobic conditions frequently resulted in rotting of the lenticels. Comparable tubers incubated in aerobic conditions showed no rotting or invasion by bacteria. The results are discussed in relation to the biological control of blackleg.     

Surface mould growth on wooden claddings – effects of transient wetting,relative humidity,temperature and material properties

ABSTRACT

Effects of climatic factors and material properties on the development of surface mould growth on wooden claddings were investigated in a laboratory experiment. Specimens of aspen (Populus tremula), Siberian larch (Larix Sibirica), American white oak (Querqus alba), Scots pine (Pinus sylvestris), Norway spruce (Picea abies) and thermally modified pine were incubated in eight climatic chambers at specified wetting periods (2 or 4?h per day), relative humidity (58–86%) and temperature conditions (10–27°C). Surface mould growth was assessed weekly for 13 weeks, and the results were evaluated statistically using Generalized Estimating Equations logistic regression models. All tested climatic factors had significant effects on the mould growth, and there were significant differences between the materials. The ranking of the materials varied with temperature and over time. Aspen, pine sapwood and oak were overall most susceptible to mould growth, and thermally modified pine least susceptible. There were significant differences between sapwood and heartwood for pine and spruce. The effect of density was tested on the spruce heartwood material, but was not found to be significant. The results can be used to further develop prediction models for mould growth on wooden claddings.     

水煮藕带护色配方优化研究

为改善水煮藕带产品在加工贮藏过程中易发生褐变的问题，本研究以新鲜藕带为原料，探究不同护色剂的护色效果。在单因素试验的基础上，以藕带色差值为评价指标，通过正交试验对护色剂配方进行优化。结果表明，4种护色剂均能有效抑制水煮藕带产品的褐变。通过正交试验法优化了水煮藕带的护色剂配方，最适合藕带的护色剂配方为：柠檬酸亚锡二钠200mg/L，EDTA二钠150mg/L，植酸125mg/L，D-异抗坏血酸钠150mg/L，其中EDTA二钠对藕带的色泽影响最大。     

杨树维管组织特异启动子的克隆与启动活性分析

启动子在基因表达调控中起关键性作用,它在很大程度上决定所控基因表达的时间、空间和强度。依据拟南芥ATH1芯片分析杨树维管形成时期特异表达基因的结果,选取了差异表达基因NST3,通过BLAST比对在杨树EST数据库(PopulusDB)中找到同源性较高的基因NAC068。以毛白杨为材料,在其基因组中克隆得到该基因5'侧翼区901 bp长的片段,命名为pProNAC068,将该片段置换pBI121载体中的CaMV35S启动子,并在84K杨中检测报告基因GUS的表达情况。经过GUS活性检测分析发现:该启动子可以控制外源基因在次生维管组织中特异表达,从而为基因工程中有目的的控制外源基因在维管组织中的表达奠定基础。     

大豆紫斑病研究进展

紫斑病是严重影响大豆产量和品质的病害之一,本文从其病原菌及毒素、鉴定方法、对产量及品质的影响等方面进行了阐述,并对其综合防治措施进行了研究探讨,为大豆的育种工作提供了一定的理论依据。     

多倍体西瓜染色体制片技术改良及其核型分析

[目的]改良多倍体西瓜染色体标本制片技术,比较多倍体西瓜常规核型分析与荧光核型分析结果,为西瓜细胞遗传学研究及辅助构建西瓜物理遗传图谱提供参考依据.[方法]以多倍体西瓜为材料,以传统染色体制片方法去壁—低渗火焰干燥法为基础,去掉其前低渗和后低渗两个步骤,合并其再固定与火焰涂片两个步骤,用吉姆萨染色剂和荧光染料DAPI分别对多倍体西瓜染色体进行常规染色和荧光染色,并进行核型分析.[结果]使用改良型酶解去壁火焰干燥法进行多倍体西瓜染色体制片,比传统去壁—低渗火焰干燥法简化了用KCl溶液前低渗、后低渗及用卡诺固定液再固定3个步骤,可缩短制片时间1.0~2.0 h,提高制片效率,获得的染色体样本数目齐全、分散良好、形态清晰.用DAPI染色比用吉姆萨染色更容易检测到细胞中期分裂相,每张玻片可缩短检测时间0.5~1.5 h;染色体的着丝点、长臂及短臂形态更清晰.[结论]采用改良染色体制片方法可提高多倍体西瓜染色体的制片效率,对该制片进行荧光核型分析比常规核型分析更准确,效率更高.