Effects of HIF-1α silencing on proliferation of rat hepatoma cells

AIM：To study the effect of hypoxia-inducible factor 1α (HIF-1α) silencing on the proliferation of hepatoma cells under hypoxia. METHODS：Rat hepatoma cell line CBRH-7919 was used in this study. Hypoxia model was established by treating the cells with cobalt chloride (CoCl2). The expression of HIF-1α was silenced by small interfe-rence RNA. Real-time RT-PCR and Western blotting were used to detect the mRNA and/or protein expression of HIF-1α, vascular endothelial growth factor (VEGF), p21 and cyclin D1 in CBRH-7919 cells under hypoxia. The proliferation of CBRH-7919 cells was measured by the technique of 5-bromo-2’-deoxyuridine (BrdU) incorporation. RESULTS：The expression of HIF-1α and VEGF at mRNA and protein levels was significantly increased under hypoxia (P<0.05). Silencing of HIF-1α significantly inhibited the expression of HIF-1α, VEGF and cyclin D1 at mRNA and/or protein levels, while increased the protein expression of p21 (P<0.05). The BrdU-positive cells in HIF-1α siRNA transfection group were significantly less than those in control group. CONCLUSION：HIF-1α silencing significantly inhibits the proliferation of hepatoma cells under hypoxia.     

能源用甜高粱辽甜1号选育报告

辽甜1号(L0201A/LTR102)是以自选不育系L0201A为母本,以LTR102为父本组配而成的能源用甜高粱杂交种。该杂交种茎秆产量高,茎秆多糖多汁,含糖锤度高,高抗丝黑穗病、叶病轻、抗倒伏,乙醇转化率高。生育期适中,作为能源作物在有效积温≥2 850℃的地区均可种植。     

CNMARC著录中1XX字段的著录分析

结合实例就IXX字段著录过程中容易出现混淆的代码进行分析，提高CNMARC记录中1XX字段的录入质量。     

Interleukin 1, a mediator of immunoadjuvant peptidoglycans

Bacterial peptidoglycans and the synthetic analog muramyl dipeptide possess various immunomodulating properties (adjuvant effect, increase of resistance to infectious agents and to tumor growth). They are able to induce B cell activation and to stimulate macrophages to produce monokines such as Interleukin 1 (IL 1). IL 1 plays an essential role in immune response. It promotes thymocytes maturation and Interleukin 2 secretion by antigen sensitive T cells, which in turn triggers regulatory T cells. Moreover, it is involved in the proliferation and differentiation of B cells.

There is a correlation between the immunoenhancing effect of PG of a definite structure and their ability to induce IL 1 secretion. Non-adjuvant PG were inactive. This suggests that one of the major mechanisms of action of adjuvant PG could be the stimulation of IL 1 synthesis.     

辣椒新品种川椒子弹头的选育

川椒子弹头是以辣椒胞质雄性不育系E16A为母本,恢复系E04C为父本配制而成的早熟辣椒一代杂种。果实锥形,纵径5～6cm,横径2.5～2.7cm,果肉厚0.2cm,平均单果质量20g。青熟果绿色,老熟果鲜红色,辣味香浓,干椒油份重。每667m2产鲜红椒2800kg左右,干椒400～450kg。田间对TMV、CMV的抗性较石柱朝天红强,适宜黄河流域、长江流域早春露地和保护地栽培。     

三氟甲基侧取代氧、氮、硫杂大环化合物的合成和分子结构

合成了两个三氟甲基侧取代氧、氮、硫杂大环化合物（１、２），用ＩＲ、＾１ＨＮＭＲ、ＭＳ和元素分析作表征；用ＡＭ１方法计算了它们的电子结构，并根据计算结果给出了几何构型。     

鲁梅克斯K-1杂交酸模简介及在我省的种植意见

简要介绍了优质高产饲草作物—鲁梅克斯K-1杂交酸模的来源、生态习性、优良性状、适口性、利用方法、适植范围及在栽培利用时应注意的问题,提出了在我省的种植意见和建议。     

ABT1号生根粉在桑树高空压条上的应用研究

桑树高空压条用ABT1号生根粉处理后的各项成绩均优于清水对照 ,以50mg/kg的浓度为最好。与对照比较 ,生根速度提早1周 ,生根数提高22.63倍 ,株根长度提高26.85倍     

龙薯1号在丘陵山地高产高效栽培技术研究

为探讨龙薯1号在丘陵山地高产高效栽培技术,2005-2006年对龙薯1号进行了栽培试验,试验采用单因子随机区组排列,重复三次进行。产量对比分析结果显示:种植密度应控制在45500~55500株/hm2;施夹心肥的效益比施夹边肥高;每公顷应施纯氮120 kg、五氧化二磷90 kg、氧化钾210 kg、硼砂15 kg;封垄时喷300kg/hm2多效唑溶液1次。