日本一项研究发现:男性多吃蔬菜可降胃癌风险

<正>据新华社电日本国立癌症研究中心的一份新报告说,大量食用蔬菜的日本男性患胃下部癌的风险明显较低,这可能是因为蔬菜中含有的抗氧化成分遏制了胃癌元凶之一的幽门螺杆菌发挥作用。这家研究中心对15万日本人进行了问卷调查,按每天的蔬菜进食量将研究对象分为5组,进行了约11年的跟踪调查。调查结果显示,上述研究对象中有1 670人患上了胃癌,     

纳米孔生物技术的新进展

纳米孔技术通过绝缘膜上的纳米级微孔随机地高通量识别溶液中的生物大分子。纳米孔检测原理的普遍性以及单分子检测的易用性,使得纳米孔在生物技术方面具有较大的应用潜力。最近关于纳米孔的结构和精密度研究取得了一定的进展,其在DNA/蛋白质绘图、生物大分子结构分析、蛋白质检测以及DNA测序方面也得到了广泛应用。     

首次报道病毒miRNA调控对虾抗病毒

<正>病毒,尤其是DNA病毒,会产生自身的microRNA(miRNA)来调控宿主和病毒基因的表达。由于其在病毒-宿主相互作用中的重要作用,近年来病毒miRNA已引起了广泛的关注与研究,然而人们对无脊椎动物病毒miRNA的了解却十分有限。大多数对病毒miRNA的研究是在细胞系中进行的,但是病毒miRNA在细胞系中发挥的作用可能与其在宿主体内的作用差异巨大。浙江大学生命科学学院章晓波教授课题组首次对对虾体内的白斑综合症病毒(WSSV)编码的miRNA进行了表征分析,     

一种高质量天门冬基因组DNA提取方法

针对天门冬[Asparagus cochinchinensis(Lour.)Merr.]成熟叶片富含多糖、多酚、蛋白质及其他次生代谢物质的特点,采用改良CTAB法对其基因组总DNA进行提取,并对DNA进行质量检测。结果表明,改良CTAB法可从天门冬成熟叶片中获得纯度高、完整性好的总DNA,其OD260 nm/OD280 nm为1.86,OD260 nm/OD230 nm为2.36,浓度为390μg/mL,产率达11.7μg/g,多糖、多酚和RNA杂质被去除干净,无降解现象,说明该方法提取的天门冬基因组总DNA质量较高。     

草果AtNCED基因克隆、表达和植物表达载体构建

9-顺式环氧类胡萝卜素双加氧酶(9-cis-epoxycarotenoid dioxygenase, NCED)是ABA生物合成的限速酶。诸多研究表明NCED基因表达量的变化与ABA含量显著相关,在种子休眠过程中发挥重要作用。为探究草果AtNCED基因的序列特征和功能,本研究以草果(Amomum tsaoko Crevost et Lemarie)种子转录组为基础,采用RT-PCR技术克隆了一个AtNCED基因。该基因全长2 372 bp,包含一个1 830 bp的开放阅读框(open reading frame, ORF),编码610个氨基酸。生物信息学分析显示,AtNCED基因编码的蛋白在N-末端包含典型的叶绿体转运肽序列,在催化结构域含有4个高度保守的组氨酸残基。AtNCED蛋白与芭蕉科马来西亚蕉同源性较高,与其他单子叶植物处在同一进化分支。实时荧光定量PCR分析结果表明,AtNCED基因表达量随着层积时间的增加逐渐下降,该基因可能正向调控种子休眠,在草果种子休眠诱导与维持中发挥重要作用。进一步地,利用同源重组方法,成功构建了pBI121-AtNCED过表达载体,并转化至农杆菌EHA105。该研究结果为弄清ABA激素信号在种子休眠中的调控作用提供了帮助。     

Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events

To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border（RB） and left border（LB） regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head（RB-to-RB） concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events.     

pIRES2-EGFP-prnd真核表达载体的构建及其在BHK细胞中的表达

为探讨叠朊蛋白(Doppel)在正常生理条件及相关疾病中的临床及生物学意义,构建BALB/c小鼠Doppel基因prnd的重组真核表达质粒pIRES2-EGFP-prnd,并转染BHK细胞,IFA检测其表达情况。结果表明,Doppel蛋白通过pIRES2-EGFP-prnd在BHK细胞中表达。试验可为进一步研究Doppel蛋白的结构、正常的生理生化功能及其与相关疾病的关系提供重要工具。     

Effect Comparison of Adenovirus Vector Transfer into Different Buffalo Cells

Buffalo mammary epithelial cell,cumulus cell and fibroblasts were transfected by adenovirus vectors and compared their transfection efficiency.293 cells were transfected with pBHGloxdelE13cre and pDC316-eGFP by liposome,the virus was collected and titer was detected.Buffalo mammary epithelial cell,cumulus cell and fibroblasts were exposed to different multiplicity of infection (MOI) of adenovirus vectors.After 72 h,the cells were observed with inverted fluorescence microscope,and transfection efficiency was calculated.When the MOI was 25,50,100,200 and 400,the transfection efficiency of fibroblasts were 0.7%,7.0%,9.0%,12.5% and 34.0%,the transfection efficiency of cumulus cells were 42.5%,55.3%,57.4%,76.0% and 80.0%,the transfection efficiency of mammary epithelial cells were 88.7%,100%,100%,100% and 100%.The results showed that the transfection efficiency of mammary epithelial cell was the best,followed by cumulus cell,and fibroblast was poor.