反相高效液相色谱法测定不同品质栀子中栀子苷的含量

建立反相高效液相色谱-光电二极管阵列检测器法测定了不同品质栀子生品中栀子苷的含量。采用Nucleodur C18色谱柱(4.6mm×250 mm,5μm),以甲醇-水(22∶78,体积比)为流动相,流速1.0 ml/min,检测波长240 nm,柱温28℃。栀子苷在0.80~7.20μg范围内呈良好的线性关系(r=0.999 98),加样回收率为99.3%。该方法简便、快速、准确,可用于栀子生品的质量控制。     

基于FPGA和ARM的新型电能质量监测系统的设计

设计了一种基于FPGA和ARM的新型电能质量监测系统。该系统在FPGA上利用FFT核和软核处理器进行数据采集和处理,通过ARM处理实现数据显示、存储、通信和实时波形显示等功能。系统采用软、硬件协同设计,便于调试与修改。经过实际的调试和运行,电路功能正常,证明了该设计的合理性和可用性。     

基于气敏传感器阵列的茶叶等级检测方法研究

采用由6个金属氧化物气教传感器组成阵列的电子鼻对3个等级的信阳毛尖茶进行检测.通过主成分分析(PCA)、系统聚类分析和线性判别分析(LDA)对茶叶样本进行了分类判别.试验结果表明,该气敏传感器阵列可以区分不同等级的信阳毛尖茶叶.PCA、系统聚类分析和LDA判别分析的正确率分别为96.6%,88.3%,100%.     

Identification of the resistance gene to powdery mildew in Chinese wheat landrace Baiyouyantiao

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most damaging diseases to wheat in the world. The cultivation of resistant varieties of wheat is essential for controlling the powdery mildew epidemic. Wheat landraces are important resources of resistance to many diseases. Mapping powdery mildew resistance genes from wheat landraces will promote the development of new varieties with disease resistance. The Chinese wheat landrace Baiyouyantiao possesses characteristic of disease resistance to powdery mildew. To identify the resistance gene in this landrace, Baiyouyantiao was crossed with the susceptible cultivar Jingshuang 16 and seedlings of parents and F₁, BC₁, F₂, and F_2:3 were tested with Bgt isolate E09. The genetic results showed that the resistance of Baiyouyantiao to E09 was controlled by a single recessive gene, tentatively designated PmBYYT. An Illumina wheat 90K single-nucleotide polymorphism (SNP) array was applied to screen polymorphisms between F₂-resistant and F₂-susceptible DNA bulks for identifying the chromosomal location of PmBYYT. A high percentage of polymorphic SNPs between the resistant and susceptible DNA bulks was found on chromosome 7B, indicating that PmBYYT may be located on this chromosome. A genetic linkage map of PmBYYT consisting of two simple sequence repeat markers and eight SNP markers was developed. The two flanking markers were SNP markers W7BL-8 and W7BL-15, with genetic distances of 3 and 2.9 cM, respectively. The results of this study demonstrated the rapid characterization of a wheat disease resistance gene and SNP marker development using the 90K SNP assay. The flanking markers of gene PmBYYT will benefit marker-assisted selection (MAS) and map-based cloning in breeding wheat cultivars with powdery mildew resistance.     

推动我省鲑鳟鱼网箱养殖提质增效的成效和对策

本文概述了2013年我省沿黄鲑鳟鱼网箱养殖技术推广工作取得的突出成效,分析了目前网箱养殖存在的突出问题,明确了2014年及今后网箱养殖技术推广工作思路。     

基于印制绕组的电磁式自供电拖拉机轮速传感器研究

针对传统供电方式难以适应现代农业传感器网络使用需求的问题,设计了一种基于印制绕组和Halbach阵列的电磁式自供电拖拉机轮速传感器。装置采用中间定子、两侧转子的结构形式,基于法拉第电磁感应定律对装置的输出感应电动势进行理论推导,确定其影响参数。使用有限元仿真软件,对影响装置输出性能的磁体排列方式、线圈匝数和装置气隙等进行仿真分析,并结合实际工程经验确定了定子线圈形状和永磁体极对数等参数。设计了后端电路,其可对装置的输出电压进行整流、测量和传输,从而计算转速,并可由装置直接供电。制作了装置样机,分别开展台架标定测试和实车应用试验。试验结果表明,装置转速和电压线性拟合结果良好,决定系数R2为0.99991,在转速400r/min下整流后的直流电压输出约为3.16V,可以满足为后端电路供电的需求。与商用编码器相比,在台架测试中装置的转速测量精度保持在1%以内,实车测试中精度基本保持在5%以内,可以满足实际使用要求。     

Design of a high performance optic to electrical array for visual recovery

A high performance optic to electrical array for visual recovery is proposed by imitating the mechanisms of photoreceptor in a human retina. The array can transform the incident natural light into a pulse signal, which pulse width is a constant and frequency is proportional to the light intensity. Design and verification of the chip has been completed in standard 0.5 μm CMOS process. Unique structures and methods are employed to optimize several key parameters and obtained higher performance. The pulse width is 0.13 ms and the pixel acquires 70 dB dynamic range, thus it can work reliably in common natural light scope. The pixel size is about 53 μm×50 μm and the power consumption is 0.62~1.46 μA per pixel. The experimental results show that the presented optic to electrical array is suitable to be high density integrated and to be wireless radio frequency powered, and therefore more suited for retinal prosthesis.     

基于单片机植物组培专用LED光源的设计

为解决植物组培对光环境的需求,依据组培苗对光的选择性吸收,突破传统的点、线光源局限,采用模块化阵列式设计理念,研制出由14R、6B、4W、1UV 1W LED组成5*5基元模块,再由若干个单元模块组成"LED阵列平面光源",其发射光谱与植物的选择性吸收光谱相匹配;采用涂覆低温红外高辐射材料的铝基顶齿散热器和轴流风扇结合的方式,降低大功率LED结温;本光源能够对光质、光强、光周期和工作方式进行动态调节。可专业用于组培育苗,并在光照培养箱、温室种植及植物工厂等方面进行科学研究和推广应用。     

微波辅助萃取蚕沙叶绿素的工艺研究

以蚕沙为原料,丙酮比乙醇为2 1的混合溶液为萃取剂,采用微波辅助法萃取蚕沙叶绿素,通过单因素试验和正交试验设计考察微波压力、微波时间、微波功率等主要工艺参数对萃取效率的影响,并优化萃取工艺。结果表明,其最优工艺条件为:微波压力为0.4 MPa,微波时间为50 s,微波功率为300 W。在此最优工艺条件下,蚕沙叶绿素a的浓度可达到14.325 mg.L-1。     

美味蘑菇原生质体制备与再生研究

采用正交试验方法对美味蘑菇原生质体制备与再生最佳体系进行筛选,包括酶条件、渗透压稳定剂、菌龄、酶解的时间及温度。结果表明,原生质体最佳制备条件为液体静置培养12d菌丝体,0.6mol·L-1NaCl作为渗透压稳定剂,在2%溶壁酶和3%蜗牛酶作用下,28℃酶解3h,原生质体制备率为3.6×106个·mL-1;最佳再生条件为液体静置培养12d菌丝体,0.6mol·L-1MgSO4·7H2O作为渗透压稳定剂,在2%溶壁酶和1%蜗牛酶作用下,35℃酶解1.5h,原生质体再生率为3.01%。