小麦根腐病菌鉴定及其生物学特性测定

分离鉴定小麦根腐病菌，并对其进行生物学特性测定，结果表明，小麦根腐病菌属于半知菌亚门平脐蠕孢属麦根腐平脐蠕孢（Bipolaris sorokiniana(Sacc.)Shoemakey)。该菌生长的最适温度为30℃，最适PH值为7，最适培养基为PDA，60℃5min为致死温度。分生孢子在水滴中才能萌发，萌发的最适PH值为7，最适温度为25℃，在供试的营养液中，以土壤浸液中萌发率最高。     

低能离子注入麻黄种子生物效应初步研究

本文初步研究了离子束注入麻黄种子后的当代生物效应 ,研究表明 :离子注入对麻黄种子的生长起良好的生物效应。注入剂量为 4× 1 0 1 6 N+ / cm2 时 ,伊吾蓝麻黄种子发芽率最高为 76 % ,相对空白增长 4 3.4 % ;在注入剂量为 4× 1 0 1 6 N+ / cm2 ,伊吾蓝麻黄种子的发芽 3天时呼吸速率达 1 .1 4 0 mg/ g· h。这也表明发芽率高的剂量下 ,种子的呼吸速率增加 ,注入剂量为 1 2× 1 0 1 6 N+ / cm2 ,伊吾蓝麻黄种子的盆栽出土率最大为 2 2 .7%。 4× 1 0 1 6 N+ / cm2 剂量离子注入和硕蓝麻黄种子时 ,其盆栽出土率最大为 4 2 .3% ,比对照增加了 6 4 %。     

珍稀濒危植物柄扁桃的花部特征比较研究

通过对2种柄扁桃花(白花和粉花)花部特征比较研究.结果表明:2种类型的花在花冠直径、花瓣长度、柱头高度等方面存在差异,而且白花的单花花粉量,花粉/胚珠比率(P/O)少于粉花.另外,白花的花粉活力和柱头可授性显著高于粉花(P0.05).     

不同木豆品种的生物学和品质特性比较研究

以广西地方木豆品种为对照,与引进的其他7个木豆新品种进行田间比较试验,评价引进木豆品种的种性和生态适应性.结果表明:不同木豆品种的根、茎、叶、花、荚、籽粒、株型等形态特征,萌芽、生长、开花、生长期、抗病虫性等生长特性以及茎、叶、籽粒的粗蛋白、粗脂肪、粗淀粉含量都有显著差异.根据不同品种的表现:广西品种、非洲品种、缅甸品种、ICPL87119比较适应喀斯特石山区荒地、山地种植,可作为饲料、水土保持、生态保护、薪炭林等用途,ICPH8、IC-PL87091可作蔬菜和粮食用,其中ICPL87091是一个比较有希望的品种.     

Different longitudinal distribution patterns of native white-spotted charr and non-native brown trout in Monbetsu stream, Hokkaido, northern Japan

Abstract  This study focused on longitudinal distribution patterns of native white-spotted charr and non-native brown trout in a mountainous stream in Hokkaido, Japan. Brown trout ratio, which is the proportion of brown trout in the catch of salmonids, was decreasing from downstream to upstream. Brown trout ratio correlated negatively with water temperature. Thus, our results suggested that temperature may influence the possible competition between native white-spotted charr and non-native brown trout.     

缓释肥减量施用对覆膜栽培玉米生物性状、干物质积累与养分分配的影响

通过大田试验研究缓释肥覆膜减量条件下的玉米生物性状、干物质分配比例、养分含量差异及其与产量的相关性分析。结果表明,与不覆膜(T2)处理相比,覆膜可使玉米的茎粗提高11.05%~19.34%,株高提高2.78%~5.39%,穗位高提高6.20%~10.28%,覆膜条件下缓释肥减量对玉米生物性状无明显影响。覆膜可以使玉米干物质积累提高25.22%~31.01%,较普通复合肥(T6)相比,施用缓释肥更有利于玉米干物质积累,且缓释肥覆膜减量15%(T4)不会影响玉米干物质积累,减量过度(T5)则会显著降低玉米干物质积累以及子粒和茎秆的氮含量。相关分析发现,玉米产量与生物性状存在显著或极显著的相关性,与不同部位养分含量也存在显著或极显著相关性。覆膜条件下施用缓释肥有利于改善玉米生物性状,提高干物质积累和养分含量,玉米茎粗、株高、穗位高与茎秆氮含量、叶片钾含量、子粒氮含量可以作为玉米产量评估的重要指标。     

Effects of CENP-W down-regulation on human glioma U87 cells

AIM: To study the effect of centromere protein W (CENP-W) down-regulation on human glioma U87 cells.METHODS: Small interfering RNA (siRNA) was used to inhibit the expression of CENP-W in the U87 cells. The interference effect of siRNA was evaluated by RT-qPCR and Western blot. The proliferation of the cells was analyzed by MTT assay, BrdU staining and colony formation experiment. Transwell chamber assay was used to detect the invasion ability of the cells. The cell migration ability was measured by a scratch test. The changes of the cell cycle distribution and apoptosis were analyzed by flow cytometry.RESULTS: The results of MTT assay, colony formation experiment and BrdU staining showed that the cell proliferation and colony formation abilities in experimental group were significantly lower than those in control group and negative control group. The results of Transwell and scratch experiments showed that the migration and invasion abilities in experimental group were weaker than those in blank control group and negative control group. The results of flow cytometry analysis showed that the cell cycle distribution in experimental group was arrested in G₀/G₁ phase. The percentage of apoptotic cells in experimental group was higher than that in control group (P<0.05).CONCLUSION: Down-regulation of CENP-W expression inhibits the proliferation, migration and invasion of human glioma cells and promotes the apoptosis of the cells, suggesting that CENP-W may be a potential target of gene therapy for human glioma.     

2016年秋冠小白菜春秋两季多点比较试验

以华冠小白菜为对照品种,在大田生产条件下对秋冠小白菜进行新品种的多季多点比较试验,以期对新选育的秋冠小白菜的生物学性状、品质、产量等进行合理评价.结果表明:在多季多点试验中,新品种秋冠小白菜株高、株幅、叶长、叶宽、叶片数等均比对照表现好,株型紧凑,春、秋季平均667 m2产量分别比对照华冠提高18.5%、25.6%;抗病性及抗逆性均比对照强;维生素C、可溶性蛋白质、可溶性糖含量均较高,综合营养品质优良.     

施用生物有机肥对水晶蜜柚品质的影响

探讨生物有机肥与‘东试早’柚果实物理品质、化学品质及风味之间的影响,为德宏瑞丽地区‘东试早’柚适宜生物有机肥施用量提供参考。以11 年树龄‘东试早’柚为试材,测定‘东试早’柚果实物理、化学品质,并对果实进行感官风味品尝鉴定。结果表明,生物有机肥施用量30 kg/株,单果重、果肉重、纵径、横径最佳,此时还原糖、总糖、可溶性固形物和Vc含量最高,感官风味评价综合满意度分数最高。生物有机肥施用量增加到40 kg/株,单果重下降,可滴定酸含量显著升高,感官风味评价综合满意度分数有所下降。相关性分析显示,生物有机肥与还原糖、总糖、Vc和感官风味评价呈显著相关;感官风味评价的综合满意度与还原糖、总糖呈极显著正相关,与可滴定酸显著负相关。综合考虑‘东试早’柚的品质,生物有机肥用量以30 kg/株为宜。     

Comparative performance of the highly invasive Mimosa invisa and its less invasive subspecies under different nutrient and light conditions

Invasive plants may be more plastic than non‐invasive plants and maintain high fitness under various environmental conditions. Previous studies mainly focused on the comparisons between invasive and native plants, and comparisons between highly invasive and less invasive exotic species are still relatively rare, especially for comparisons at the subspecies level. This study examined the effects of nutrient addition and shading on the performance of the highly invasive Mimosa invisa and its less invasive subspecies M. invisa var. inermis under either isolated or competitive conditions. Nutrient addition increased biomass and plant height and decreased root‐to‐shoot ratio (R/S). Shading decreased biomass and R/S and increased plant height. Under isolated conditions, the two invaders did not differ in R/S, plant height and plasticity of these traits in response to nutrient addition or shading, and the two invaders also did not differ in biomass production under each of the nutrient and light treatments. When the two invaders competed with each other, M. invisa outcompeted M. invisa var. inermis under high soil nutrient conditions, and the two invaders did not differ in performance under other growth conditions. Thus, only considering competition may we find out the difference between highly invasive species and their closely related, less invasive subspecies. Management of M. invisa should focus on habitats with high soil nutrient availability, in which M. invisa is more likely to dominate.