在太湖地区乌栅土上,采用田间小区试验连续两年研究了施氮(N)量为0、180、255、330kg hm-2,施磷(P2O5)量为0、309、0、180 kg hm-2的6个组合(对照N0P0、低氮N180P90、优化N255P90、低磷N255P30、高磷N255P180、高氮N330P90)以及三个施肥时期对稻田氨挥发损失的影响,氨挥发采用密闭室间歇通气法测定。结果表明,稻田氨挥发损失主要发生在施肥后6d内,基肥和第一次追肥后各处理氨挥发量占施氮量的0.4%~11.5%,而第二次追肥后氨挥发损失比例较大,对照、低氮、优化、低磷、高磷和高氮处理的氨挥发在2002年稻季分别占施氮量的5.8%、9.7%、25.6%、15.6%和11.6%,在2003年稻季则分别为27.4%、26.2%、30.0%、35.1%和27.6%。若施肥后遇阴雨天气或正值水稻拔节孕穗期,氨挥发量便降低。田面水中的NH4 -N浓度是氨挥发的决定因素之一,与氨挥发通量呈正相关。施磷量相同时,氨挥发随施氮量增加而增加;施氮量相同时,高磷和低磷处理氨挥发均高于优化处理,表明在氮磷不平衡施用时,氮肥氨挥发损失会加剧,从氨挥发损失方面考虑,稻田推荐施磷量不宜超过P2O590 kg hm-2。 相似文献
Dried soil samples from many sources have been stored in archives world-wide over the years, but there has been little research on their value for studying microbial populations. Samples collected since 1843 from the Broadbalk field experiment on crop nutrition at Rothamsted have been used to document changes in the structure and composition of soils as agricultural practices evolve, also offering an invaluable record of environmental changes from the pre- to post-industrial era in the UK. To date, the microbial communities of these soils have not been studied, in part due to the well-documented drop in bacterial culturability in dried soils. However, modern molecular methods based on PCR amplification of DNA extracted directly from soil do not require bacterial cells to be viable or intact and may allow investigations into the legacy of bacteria that were present at the time of sample collection.
In a preliminary study, to establish if dried soils can provide a historical record of bacterial communities, samples from the Broadbalk soil archive dating back to 1868 were investigated and plots treated with either farmyard manure (FYM) or inorganic fertilizer (NPK) were compared. As anticipated, the processes of air-drying and milling greatly reduced bacterial viability whilst DNA yields declined less and may be preserved by desiccation. A higher proportion of culturable bacteria survived the archiving process in the FYM soil, possibly protected by the increased soil organic matter. The majority of surviving bacteria were firmicutes, whether collected in 2003 or in 1914, but a wide range of genera was detected in DNA extracted from the samples using PCR and DGGE of 16S rRNA genes. Analysis of DGGE band profiles indicated that the two plots maintained divergent populations. Sequence analysis of bands excised from DGGE gels, from a sample collected in 1914, revealed DNA from - and β-proteobacteria as well as firmicutes. PCR using primers specific for ammonia oxidizing bacteria showed similar band profiles across the two treatments in recently collected samples, however older samples from the NPK plot showed greater divergence. Primers specific for the genus Pseudomonas were designed and used in real-time quantitative PCR to indicate that archived soil collected in 1868 contained 10-fold less pseudomonad DNA than fresh soil, representing around 105 genomes g−1 soil. Prior to milling, dramatically less pseudomonad DNA was extracted from recently collected air-dried soil from the NPK compared to the FYM plot; otherwise, the two plots followed similar trends. Overall bacterial abundance, diversity and survival during the archiving process differed in the two soils, possibly due to differences in clay and soil organic matter content. Nevertheless, the results demonstrate that air-dried soils can protect microbial DNA for more than 150 years and offer an invaluable resource for future research. 相似文献
