一个普通小麦-大赖草易位系T01的选育与鉴定

将抗赤霉病的普通小麦-大赖草Lr.2染色体单体异附加系花粉经过60Co-γ射线处理, 然后给感赤霉病的普通小麦品种“扬麦5号”授粉, 杂交后代连续2年进行赤霉病抗性单株接种鉴定, 从中选育出一个普通小麦-大赖草异易位系。 经过染色体荧光原位杂交和Giemsa C-分带鉴定, 易位发生在普通小麦4B染色体长臂和大赖草第2条染色体(L     

基于B/S模式的奶牛日粮诊断系统的实现

针对现有奶牛日粮诊断过程中只考虑日粮营养组分方面的因素造成的局限性,本文首次提出了将奶牛日粮营养组分与营养代谢性病两个因素综合考虑,并以此结果对日粮配方优化设计。基于web2.0技术,采用JSP+Tomcat+MySQL架构,建立了B/S（浏览器/服务器）模式的奶牛日粮诊断系统。该系统先进实用,能有效地解决奶牛日粮配方针对性不强、难以规避奶牛营养性疾病等问题。     

白菜S位点糖蛋白基因的克隆与表达分析

 以白菜自交不亲和系为材料,采用RT-PCR技术,用特异引物对SLG基因进行克隆,获得SLG基因cDNA序列长度为1 324 bp,命名为BcSLG。序列分析表明：所获得的白菜BcSLG基因cDNA序列包含一完整的编码框,编码432个氨基酸,含有12个保守的半胱氨酸残基和7个N-糖基化位点。序列比对和系统进化分析表明：BcSLG与其它植物的SLG基因氨基酸序列具有较高的同源性,与大白菜和甘蓝亲缘关系最近。荧光定量PCR分析表明：BcSLG基因在自交不亲和系的柱头中表达量最高,其次是花蕾,叶片中表达最低, 在自交亲和系的柱头、花蕾和叶片中相对表达较低。     

虎掌菌酶解提取物抑菌试验研究

采用木瓜蛋白酶、纤维素酶对虎掌菌进行酶解提取,并检测提取物对大肠杆菌、金黄色葡萄球菌、酵母菌、青霉菌和黑曲霉的抑菌效果。结果表明,不同提取方法所得粗提物都有不同程度的抑菌效果,提取液对黑曲霉和青霉菌的抑菌效果最明显,对酵母菌抑菌效果最不明显。     

Genetic analysis of Aequipecten opercularis and Mimachlamys varia (Bivalvia: Pectinidae) in several Atlantic and Mediterranean localities, revealed by mitochondrial PCR-RFLPs: a preliminary study

Aequipecten opercularis (Queen scallop) and Mimachlamys (Chlamys) varia (Black scallop) are important natural resources occurring in Atlantic and Mediterranean coasts. To develop an optimal sustainable exploitation plan, it is important to study the genetic structure of the different populations. In this study, we used polymerase chain reaction‐restriction fragment length polymorphisms for the determination of the genetic variation and population structure of these two species in different localities. Ten composite haplotypes were generated for A. opercularis and 15 haplotypes for M. varia. Of these, six and four were unique respectively. The analysis of the distribution of the different haplotypes between the localities showed no clear evidence of subdivision in A. opercularis, while in M. varia the results indicated that the two localities analysed should be managed as separate stocks.     

大连湾和辽东湾夏季海水细菌多样性16S rDNA分析

通过构建大连湾(DL17)和辽东湾(ZD-LDW017)2个站点夏季海水的细菌16s rDNA文库,利用16S rDNA序列比对法,对大连湾和辽东湾夏季海水细菌多样性进行了分析.试验结果表明,这2个海域的细菌主要为非培养细菌.每个海区分别随机选取了200个克隆进行酶切谱型分析和序列测定,共得到104个不同的DNA序列,其中大连湾51个序列,分别属于13种(属);辽东湾53个序列,分别属于13种(属).有6种菌为这2个海区所共有,这2个海区各自有不同的优势菌,且均未检测到致病菌,研究结果表明大连湾和辽东湾海水细菌具有丰富的多样性.     

车用28V高性能铁氧体永磁发电机的设计

对车用28V铁氧体永磁发电机进行了结构设计,确定出永磁体体积及发电机转子和定子的结构参数,设计了由基准电路、取样电路、触发电路等组成的三相半控桥式整流稳压电路,对设计的发电机及稳压电路进行了试验分析,验证了设计的合理性.     

基于AT89S51的汽车智能安全报警系统

利用AT89S51设计了一种新型汽车防盗报警系统.当满足报警条件,单片机会向GSM模块发出指令,系统向机主终端发出报警求助短信,同时还发出声光报警、切断发动机电源.本系统具有误报率小,可靠性高,成本低,使用方便等优点.     

施氮水平对7S亚基缺失大豆根系形态和结瘤固氮的影响

为有效推广功能型大豆7S亚基缺失品种,以7S亚基缺失大豆品系东富2号为研究对象,设置4种施氮水平(纯N),N0(0 mg·kg~(-1))、N1(25 mg·kg~(-1))、N2(50 mg·kg~(-1))、N3(75 mg·kg~(-1)),采用桶栽法研究大豆根系形态和结瘤固氮对不同施氮水平的响应。结果表明:N1(25 mg·kg~(-1))水平下根系干重加大,根冠比增大,根瘤固氮潜力高,单株产量较高。N2(50 mg·kg~(-1))水平下根长、根表面积、根体积在生育后期增长较快,根系干重较大,根冠比低,固氮酶活性最高,单株籽粒产量最高。N3(75 mg·kg~(-1))水平下植株干重较大,无效生长较多,根瘤数少,固氮潜力和根冠比低,单株产量不高。综合籽粒产量和根系特性指标,功能型大豆7S亚基缺失品系东富2号的适宜施肥量为25~50 mg·kg~(-1)。     

Interspecific cross‐hybrids of Nicotiana tabacum L. cv. (gla.) S ‘K326’ with Nicotiana alata

In order to introduce the Tomato Spotted Wilt Virus (TSWV) resistance from Nicotiana alata into Nicotiana tabacum, a cytoplasmic male sterility (CMS) line of N. tabacum (N. tabacum L. cv. (gla.) S ‘K326’), was successfully crossed with N. alata. Despite a high DNA content variability, F₁ hybrids could be classified in two subgroups, a major one encompassing fertile hybrids morphologically similar to their tobacco maternal parent but TSWV sensitive, and a minor one displaying sterile hybrids showing an intermediate phenotype and TSWV resistant. In order to elucidate the unexpected fertility recovery of the fertile F₁ plants, some N. alata fertility restoration ppr genes were cloned and were shown to be differentially expressed between parental lineages as well as between both F₁ subgroups, suggesting that N. alata contains fertility restoring allele able to overcome the CMS of N. tabacum.