苏玉19优质高产关键制种技术研究

苏玉19是江苏沿江地区农科所以自选系T458作母本,自选系T877作父本,杂交育成的高产优质早熟大穗型饲用玉米新品种.为提高制种产量,保证种子质量,通过观察亲本自交系特征特性,分析各种影响制种产量和质量的单项因素,结合制种实践,进行组装配套,形成苏玉19优质高产制种配套技术.     

玉米品种‘新科19’花期调节制种技术的研究

花期相遇良好是确保制种成功的关建,通过进行玉米杂交种‘新科19’双亲的播期试验及雌、雄穗分化观察,掌握了2个自交系的穗分化及吐丝、散粉规律,明确了‘新科19’正交制种播期调节内在原因及花期预测的叶龄指标。     

油用向日葵新葵19号恢复系654R的选育及利用

[目的]研究油用向日葵恢复系的选育方法。[方法]在现有技术中向日葵恢复系的选择过程比较漫长且结果不明确的情况下,为了改变从杂种一代F1以后连续自交的作法,在早代就开始进行测定其配合力,有目的的选择,方向比较明确,提高选择效率,创造新的优异资源。[结果]选育出油用向日葵新葵19号恢复系654R,该恢复系植株性状好、抗病性好、抗逆性强、配合力高、杂种优势强。[结论]该研究提供了一种油用向日葵恢复系新的选育方法,并对654R进行利用。     

25％氰烯菌酯悬浮剂防治水稻恶苗病的田间药效

[目的]明确新型杀菌剂25%氰烯菌酯悬浮剂对水稻恶苗病的防治效果,为黑龙江省水稻生产中有效防治恶苗病提供参考。[方法]采用田间药效试验方法,评价25%氰烯菌酯悬浮剂对水稻的安全性及对恶苗病的防治效果。[结果]25%氰烯菌酯悬浮剂0.25ml/kg种子或25%氰烯菌酯悬浮剂0.15 ml/kg种子与种衣剂混用对水稻安全且对水稻恶苗病防治效果较好,明显好于对照药剂咪鲜胺和单独使用种衣剂。[结论]建议在黑龙江省水稻生产中推广应用25%氰烯菌酯悬浮剂。     

Construction of siRNA-survivin and GRIM-19 coexpression plasmid and its growth inhibitory effect on prostatic cancer DU145 cells

AIM: To construct the recombinant plasmid that expresses siRNA-survivin and GRIM-19 simultaneously, and to identify the validity of the recombinant plasmid and observe its effect on expression of survivin and GRIM-19 and proliferation ability of prostate cancer DU145 cells. METHODS: The recombinant plasmid coexpressing siRNA-survivin and GRIM-19 was constructed using gene cloning technique. The prostatic cancer DU145 cells were transfected with the coexpression plasmid and control plasmids. Survivin and GRIM-19 mRNA expression was detected by semi-quantitative RT-PCR. The proliferation ability affected by coexpression plasmid was measured by MTT assay. RESULTS: The coexpression plasmid pGRIM-19-si-survivin was successfully constructed according to DNA recombinant technique and identified through restriction enzyme digestion and plasmid sequencing. Compared with the mock, survivin mRNA expression levels were 0.55?0.05,0.62?0.08 and 0.35?0.05 in psi-survivin, pGRIM-19 and pGRIM-19-si-survivin groups, respectively. Compared with psi-survivin and pGRIM-19, pGRIM-19-si-survivin inhibited survivin mRNA expression markedly (P<0.05), while the expression levels of GRIM-19 mRNA were 1.93?0.14, 2.57?0.20 and 4.12?0.21 in psi-survivin, pGRIM-19 and pGRIM-19-si-survivin groups, respectively (P<0.01). Compared with pGRIM-19 group, pGRIM-19-si-survivin enhanced GRIM-19 mRNA expression more obviously (P<0.05). After transfection for 48 h, the proliferation rates were 58.0%?7.2%, 62.1%?6.1% and 50.2%?4.8% in the 3 experiment groups compared with the mock (P<0.05). After transfection for 72 h, the proliferation rate were 43.4%?4.3%, 51.3%?6.7% and 26.8%?7.1% in experiment groups compared with the mock (P<0.05). Compared with psi-survivin and pGRIM-19, pGRIM-19-si-survivin significantly inhibited the cell growth (P<0.05). CONCLUSION: Transfection of coexpression plasmid pGRIM-19-si-survivin dramatically changes the mRNA expression of survivin and GRIM-19 and inhibits the cell growth.     

Antinociceptive and antiplasmodial activities of cassane furanoditerpenes from Caesalpinia volkensii H. root bark

The chloroform and ethyl acetate extract (100 mg/kg) of Caesalpinia volkensii H. exhibited significant (P ≤ 0.05) antinociceptive activities using hot plate and writhing tests in mice while the later showed antiplasmodial activity (IC₅₀ 0.23 ± 0.07 and 4.39 ± 2.49 μg/ml) against chloroquine sensitive (D6) and chloroquine-resistant (W2), respectively. Two new furanoditerpenes [rel. 1β,5α-dihydroxyvoucapane (1) and rel. 1β,6β-dihydroxyvoucapane; 19β-methyl ester (2)] together with seven known compounds [voucapane (3), voucapan-5-ol (4), deoxycaesaldekarin C (5), caesaldekarin C (6), 5-hydroxyvinhaticoic acid (7), triacontanyl-(E)-ferulate (8), triacontanyl-(E)-caffaete (9) and 30′-hydroxytriacontanyl-(E)-ferulate (10)] were isolated from the two extracts. The administration of 3, 4, 5 and 6 (100 mg/kg i.p) caused a significant (P ≤ 0.05) reduction in the number of writhing episodes induced by acetic acid and (P ≤ 0.01) increased pain latency threshold in hot-plate test compared to control. However, the pure compounds indicated relatively (P ≤ 0.05) low antiplasmodial activity. The phytochemical constituents from the root bark of C. volkensii had better analgesic properties than antimalarial properties, justifying the use of the plant root bark as a remedy for pain.     

华南型黄瓜新品种吉杂19的选育

吉杂19是以自交系04196为母本,以自交系Ⅱ-0407-1-2-1-1为父本配制而成的保护地华南型黄瓜一代杂种.早熟,第1雌花节位为第4节左右,播种至始收60 d(天)左右;植株生长势较强,主侧蔓均可结瓜,结成性好;瓜条棒状、顺直,刺瘤稀少,瓜皮嫩绿色,瓜长22 cm左右,横径4 cm左右,平均单瓜质量150 g;田...     

大豆GmRLP19基因克隆及胁迫应答模式分析

以大豆品种Williams82为试验材料,克隆GmRLP19基因,通过生物信息学分析qPCR检测探讨该基因不同生育期品种、不同组织、不同非生物胁迫下表达模式。结果表明,该基因编码区全长3 138 bp,编码1 045个氨基酸,编码蛋白具有胞外信号肽,LRRNT域,富含亮氨酸重复结构域,跨膜结构域和一段胞内短肽,属于受体类蛋白家族,定位于质膜。该基因在不同生育期品种间差异表达,在豆和豆荚中特异性表达,且在5种非生物胁迫中均有响应。研究为探究WRKY20基因功能及挖掘和完善基因调控通路奠定基础。     

High protection of mice against Brucella abortus by oral immunization with recombinant probiotic Lactobacillus casei vector vaccine,expressing the outer membrane protein OMP19 of Brucella species

Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. Vaccination is the most effective way to control brucellosis. The recombinant vector vaccines are promising candidates for immunization in humans and animals. In this study, the gene encoding OMP19 antigen was primarily amplified and cloned into an expression vector called pT1NX, and then transformed to L. casei cell via electroporation technique. The expression was confirmed using specific antibody against the recombinant protein via immunological screening tests such as western blot and immunofluorescence assay. Finally, recombinant L. casei was orally fed to mice and the results were further recorded, indicating that the mice group which received OMP19 through L. casei based vaccine represented a very good general and mucosal immune responses protective against challenges with virulent B. abortus 544 strain compared with negative control recipient groups. Therefore, the vaccine produced in this research plan can be a very good candidate for protection against brucellosis.